LOCUS       sequence-275431-       12345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705312114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-06-27- 27"; volume: "114"; issue: "26"; pages: 
            "E5197-E5206"
COMMENT     Sequence Label: pLV-puro-N-3HA-SREBP2-C-Flag
FEATURES             Location/Qualifiers
     source          1..12345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1810..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2130..3312
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2361..3303
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3260..3280
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3351..3377
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3384..3410
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3414..3440
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6852..6875
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6976..7549
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6999..7551
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7143..7160)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7370..7389
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7583..8182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7583..7602)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8079..8099
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8197..8785
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8250..8270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8668..8679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8872..9105
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9177..9298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9214..9233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9268..9287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9338..9473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9493..9512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9494..9512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9522..9539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9522..9538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9531..9553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9680..10135
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9767..9786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9977..9998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10161..10265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10266..10334,10335..11126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10484..10503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11297..11885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11786..11805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12039..12056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12173..12194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12209..12214,12215..12232,12233..12239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12247..12263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12252..12274
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12271..12287
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12271..12287
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12306..12326
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12308..12326
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcttgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttt tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctgagctcc ttaaggttaa cgccaccatg tacccttatg
     3361 acgtacctga ctatgctggc gcctaccctt atgacgtacc tgactatgct ggataccctt
     3421 atgacgtacc tgactatgct ggatctagag aattcggatc catggatgag agcagcgagc
     3481 tgggcgttct ggagaccatg gagaccctca cggagctggg cgatgagctg actctcgggg
     3541 acatcgacga gatgctacag tttgtcagca atcaagtggg agagttccct gatttgtttt
     3601 cagagcaact ttgtagctcc ttccctggag gtggcagcaa tggtggcagt ggcaacaaca
     3661 gcagtggcag aggcaacaat ggtggagcca cagaccctgc agtacagcgg tcattcagcc
     3721 aggtcccatt atctaccttc tctccctcag ctgcctcccc tcaggctccg gccctgcagg
     3781 tcaaagtctc tcctacccca ccaagggcaa cccctgttct ccagccgcga ccccagcccc
     3841 agccccaacc tccagctcag ctgcagcagc agacggtgat gatcaccccg acgttcagca
     3901 ccgctccgca gacgaggatc atccagcagc ctttgatata ccagaacgca gcgaccagct
     3961 ttcaagtcct gcagcctcaa gtgcaaagcc tcgtgacgtc cccgcaggtg cagcccgtca
     4021 ccatccagca gcaggtgcag acggtacagg cccagcgggt gttgacgcag acagccaatg
     4081 gcacactgca gacccttgcc cccgccacgg tacagacggt cgctgcgcca caggtgcagc
     4141 aggtcccggt cctggtgcaa cctcagatca ttaagacaga ttcccttgtg ttgaccacgc
     4201 tgaagacaga tggcagccct gtcatggctg cagtccagaa cccggccctc acggccctca
     4261 ctgcccccat ccagacagcc gcccttcaag tgccaaccct ggtgggcagc aacgggacca
     4321 ttctgaccac aatgcctgtg atgatggggc aagagaaagt tcctatcaag caagtgcctg
     4381 gcggcgtgaa gcagctggat cctcccaaag aaggagagag gcggacaaca cacaatatca
     4441 ttgaaaagcg ctaccggtcc tccatcaacg acaaaatcat agagttgaag gacttagtca
     4501 tggggacaga tgccaagatg cacaagtctg gcgttctgag gaaggccatt gattacatca
     4561 aatatctgca gcaggtcaat cacaagctgc gccaggagaa catggtgctg aagctggcca
     4621 atcagaaaaa caagctcctg aagggcatcg acctgggcag tctggtggac agtgatgtgg
     4681 acttgaaaat tgatgacttt aaccagaatg tccttctgat gtctccgccg gcctccgact
     4741 ccgggtccca ggccggcttc tctccctatt ccattgactc tgagccgggc agccctctgc
     4801 tggatgacgc aaaggtcaag gatgaaccgg actctcctcc tgtggcactg ggcatggtgg
     4861 accgctctcg aatcctctta tgtgtcctca ccttcctggg cctctccttt aaccccttga
     4921 cttccttgct gcagtgggga ggggcccaca acactgacca gcacccatac tcaggctcgg
     4981 gccgcagtgt actgtcactg gagtcaggtg ctgggggctg gtttgactgg atggttccaa
     5041 ctctcctcct gtggctggta aatggtgtga ttgtcttgag cgtctttgtg aagctgttgg
     5101 tccacgggga gccggtgatt cgcccacact cacgcccctc agtcaccttc tggagacacc
     5161 ggaagcaggc agacctagac ctcgccaaag gtgatttcgc agctgctgct gccaacctac
     5221 aaacctgctt atccgtgctg ggccgggcgc tgcccacctc ccgcctggac ttggcctgca
     5281 gcctctcctg gaatgtgatc cgctacagcc tgcagaagct gcgcctggta cgctggttac
     5341 tcaagaaggt cttccagcgc tggcgggcta ctcccgccac tgcagctggc tttgaggatg
     5401 aggctaagag cagcgcaagg gatgcggcac tggcctacca caggctgcac cagctgcaca
     5461 tcacagggaa gcttccggcg ggatctgcct gttcagatgt gcacatggct ctgtgtgctg
     5521 tgaacctggc cgagtgtgcg gaggagaaaa tcctaccaag cacactgatt gagatccacc
     5581 tgacagcggc catggggctc aagacccgct gtggaggcaa gctgggcttc ttggctagct
     5641 acttcttaaa tcgtgcccag agtctgtgtg gcccagagca tagcactgtc cccgactccc
     5701 tgcgctggct atgccatccc ttgggccaga agtttttcat ggagcgaagc tggtcgatca
     5761 agtcagcagc caaggagagc ctgtactgcg cccagaggag cccagctgac cccattgccc
     5821 aggtacacca ggcgttctgc aaaaacctgc tagaaagagc ggtggagtcc ttggtgaaac
     5881 ctcaggccaa gaagaaggca ggcgaccagg aagaagagag ctgtgaattt tccagtgctc
     5941 tagagtattt gaaattactt cattcttttg tggactctgt gggatttgtg acctcaccat
     6001 tctccagcag ttccgtgctc aggtcggccc ttggccccga tgtcatctgc cggtggtgga
     6061 cgtccgcagt caccatggcc atcagctggc tccagggaga cgatgcagct gtgcgctctc
     6121 gttttactga agtagagcgg gtccccaagg ccctggaagt gaccgagagt cccttggtga
     6181 aggccgtctt ctatacctgt agagccatgc atgcctccct ctctgggaaa gctgacgggc
     6241 agcagaactc cttctgtcac tgtgagaggg ccagtggtca cctgtggagc agtctcaacg
     6301 tcagcggcac tacttctgat ccttcactta accatgtgat ccagctgttc acctgtgacc
     6361 tgctactgtc gctgaggaca gcactctggc aaaagcaggc cagtgccagc cagctcctgg
     6421 gtgagaccta ccatgcatcc ggcactgagc tggccggctt ccagcgggac ctgggcagcc
     6481 ttcgaaggct ggcccatagc ttccgcccag cctaccgcaa ggtgttcctg catgaagcca
     6541 ctgtgcgcct gatggcagga gccagcccta cccgtacaca ccagctgctg gagcatagcc
     6601 tacggcggcg ccccactcag aacaccaagc atggagaggt agacacctgg cctggccaac
     6661 gagagcgagc tactgccatc ctgctggcct gccgccatct tcccctctct ttcctgtctt
     6721 ccccgggcca gcgggcagtg ctgctggctg aagctgcccg caccctggag aaggtgggtg
     6781 accgccgttc ctgcagtgac tgccagcaga tgattgtcaa gctgggcggt ggcactgcca
     6841 tcgctgcctc cgactacaaa gacgatgacg acaagtgacc cgggctcgag ccatggaagc
     6901 ttgatatcta actgactgaa ccggtggtac cgatccacgc gtctccggcc tagggataac
     6961 agggtaatcc gctagcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     7021 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     7081 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     7141 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     7201 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     7261 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     7321 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     7381 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     7441 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     7501 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     7561 tagagatcca ccggtcgcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7621 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7681 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7741 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7801 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7861 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7921 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7981 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8041 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     8101 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8161 gacccgcaag cccggtgcct gagcggccgc gtcgacaatc aacctctgga ttacaaaatt
     8221 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8281 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8341 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8401 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8461 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8521 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8581 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     8641 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8701 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8761 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     8821 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8881 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8941 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9001 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9061 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9121 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9301 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9421 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9481 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9661 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9901 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9961 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10021 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10081 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    10141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10261 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10321 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10381 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10441 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10501 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10561 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10621 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10681 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10741 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10801 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10861 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10921 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10981 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11041 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11101 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11941 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    12001 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    12061 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    12121 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12181 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12241 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12301 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgc
//
