LOCUS       sequence-27548-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY7
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..81
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(85..87,88..90,91..603)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(130..151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(391..410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     738..759
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttaactttaa gaaggagata tactatgggc agcagccatc atcatcatca tcacagcagc
       61 ggcctggtgc cgcgcggcag ccatatggtg agcaagggcg aggagctgtt caccggggtg
      121 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      181 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      241 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      301 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      361 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      421 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      481 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
      541 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
      601 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      661 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      721 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      781 ggcatggncg a
//
