LOCUS       sequence-27549-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY8
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     98..119
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(366..387)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
       61 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      121 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtgct acctccggat
      181 ccaccactac caccggatcc accagatcca ccggatccac cactaccacc ggatccacca
      241 gatccaccgg atccacctga accaccggat ccaccactac caccggatcc acctgatcca
      301 ccggatccac cacgaattcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg
      361 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      421 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      481 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      541 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      601 atgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      661 ttcagctcga tgcggttcac caggg
//
