LOCUS       sequence-275516-        9627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon optimized 
            ABEmax(7.10) A-to-G base editor with 
            SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Walton RT, Christie KA, Whittaker MN, Kleinstiver BP
  TITLE     Unconstrained genome targeting with near-PAMless engineered 
            CRISPR-Cas9 variants.
  JOURNAL   Science. 2020 Apr 17;368(6488):290-296. doi: 
            10.1126/science.aba8853. Epub 2020 Mar 26.
  PUBMED    32217751
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aba8853"; journalName: "Science"; date: 
            "2020-04-17- 17"; volume: "368"; issue: "6488"; pages: "290-296"
COMMENT     Sequence Label: pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        126..505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        506..709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     660..680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     751..770
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        751..769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             819..839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2028..6128
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6171..6191
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6201..6257
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6258..6260,6261..6263,6264..6974)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6258..6260,6261..6263,6264..6974)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6564..6583)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6911..6932
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6987..7007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7016..7033
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7056..7073)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7062..7286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7357..7373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7357..7373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7370..7392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7381..7397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7405..7411,7412..7429,7430..7435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7450..7471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7588..7605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7759..8347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7839..7858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8518..9309,9310..9378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9141..9160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9379..9483)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9562..9581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
       61 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      121 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      181 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      241 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      301 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      361 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      421 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      481 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      541 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      601 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      661 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
      721 ccgtcagatc cgctagagat ccgcggccgc taatacgact cactataggg agagccgcca
      781 ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag cggaaagtct
      841 ctgaagtcga gtttagccac gagtattgga tgaggcacgc actgaccctg gcaaagcgag
      901 catgggatga aagagaagtc cccgtgggcg ccgtgctggt gcacaacaat agagtgatcg
      961 gagagggatg gaacaggcca atcggccgcc acgaccctac cgcacacgca gagatcatgg
     1021 cactgaggca gggaggcctg gtcatgcaga attaccgcct gatcgatgcc accctgtatg
     1081 tgacactgga gccatgcgtg atgtgcgcag gagcaatgat ccacagcagg atcggaagag
     1141 tggtgttcgg agcacgggac gccaagaccg gcgcagcagg ctccctgatg gatgtgctgc
     1201 accaccccgg catgaaccac cgggtggaga tcacagaggg aatcctggca gacgagtgcg
     1261 ccgccctgct gagcgatttc tttagaatgc ggagacagga gatcaaggcc cagaagaagg
     1321 cacagagctc caccgactct ggaggatcta gcggaggatc ctctggaagc gagacaccag
     1381 gcacaagcga gtccgccaca ccagagagct ccggcggctc ctccggagga tcctctgagg
     1441 tggagttttc ccacgagtac tggatgagac atgccctgac cctggccaag agggcacgcg
     1501 atgagaggga ggtgcctgtg ggagccgtgc tggtgctgaa caatagagtg atcggcgagg
     1561 gctggaacag agccatcggc ctgcacgacc caacagccca tgccgaaatt atggccctga
     1621 gacagggcgg cctggtcatg cagaactaca gactgattga cgccaccctg tacgtgacat
     1681 tcgagccttg cgtgatgtgc gccggcgcca tgatccactc taggatcggc cgcgtggtgt
     1741 ttggcgtgag gaacgcaaaa accggcgccg caggctccct gatggacgtg ctgcactacc
     1801 ccggcatgaa tcaccgcgtc gaaattaccg agggaatcct ggcagatgaa tgtgccgccc
     1861 tgctgtgcta tttctttcgg atgcctagac aggtgttcaa tgctcagaag aaggcccaga
     1921 gctccaccga ctccggagga tctagcggag gctcctctgg ctctgagaca cctggcacaa
     1981 gcgagagcgc aacacctgaa agcagcgggg gcagcagcgg ggggtcagac aagaagtaca
     2041 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2101 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2161 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2221 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2281 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     2341 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     2401 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     2461 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     2521 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     2581 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     2641 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     2701 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     2761 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     2821 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     2881 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2941 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3001 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3061 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3121 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3181 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3241 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3301 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     3361 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     3421 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     3481 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     3541 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     3601 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     3661 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     3721 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     3781 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     3841 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3901 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3961 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4021 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4081 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4141 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4201 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4261 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4321 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     4381 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     4441 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     4501 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     4561 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     4621 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     4681 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     4741 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     4801 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     4861 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4921 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4981 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5041 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5101 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5161 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5221 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5281 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     5341 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     5401 gggaccctaa gaagtacggc ggcttcctgt ggcccaccgt ggcctattct gtgctggtgg
     5461 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     5521 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     5581 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     5641 tggaaaacgg ccggaagaga atgctggcct ctgccaagca gctgcagaag ggaaacgaac
     5701 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     5761 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     5821 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     5881 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5941 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6001 actttgacac caccatcgac cggaagcagt acagaagcac caaagaggtg ctggacgcca
     6061 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6121 gaggtgactc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     6181 agaggaaagt cggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg
     6241 aggagaaccc tggacctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6301 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6361 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6421 ccgtgccctg gcccaccctc gtgaccaccc tgacctatgg agtgcagtgc ttcagccgct
     6481 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6541 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6601 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6661 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6721 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     6781 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6841 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     6901 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     6961 acgagctgta caagtctggt ggttctccca agaagaagag gaaagtctaa ccggtcatca
     7021 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     7081 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7141 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7201 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7261 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat
     7321 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta
     7441 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7501 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7561 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7621 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7681 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7741 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7801 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7861 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7921 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7981 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8041 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8101 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8161 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8221 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8281 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8341 atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga acgaaaactc
     8401 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8461 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8521 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8581 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8641 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8701 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8761 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8821 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8881 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8941 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9001 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9061 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9481 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga
     9541 tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9601 gctccctgct tgtgtgttgg aggtcgc
//
