LOCUS       sequence-275528-        8221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8221)
  TITLE     gamma adaptin
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1552 pcDNA3 HA gamma adaptin 1
FEATURES             Location/Qualifiers
     source          1..8221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             904..930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             904..930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             910..936
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(3774..3792)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3775..3792)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3812..3829)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3818..4042
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4088..4516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4175..4194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4385..4406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4525..4545)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4530..4859
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4710..4845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4772..4791
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4926..5720
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4980..4999)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5590..5609
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5894..6015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5931..5950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5985..6004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6064..6080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6064..6080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6077..6099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6088..6104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6112..6118,6119..6136,6137..6142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6157..6178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6295..6312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6466..7054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6546..6565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7225..8016,8017..8085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7848..7867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8086..8190)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccatg
      901 gcctacccct acgacgtgcc cgactacgcc tccctcggat ccaccatgcc agcccccatc
      961 agattgcggg agctgatccg gaccatccgg acagcccgaa cccaagctga agaacgagaa
     1021 atgatccaga aagaatgtgc tgcaatccgg tcatctttta gagaagaaga caatacatac
     1081 cgatgtcgga atgtggcaaa attactgtat atgcacatgc tgggctaccc tgctcacttt
     1141 ggacagttgg agtgcctcaa gcttattgcc tctcaaaaat ttacagacaa acgcattggc
     1201 tatttagggg caatgctgct gttagatgaa agacaagatg tccatcttct catgaccaac
     1261 tgtatcaaga atgatcttaa tcatagcacg caattcgtac aggggttagc actttgtacc
     1321 ctcggctgca tgggctcctc agagatgtgc agagatcttg caggagaggt agagaagctc
     1381 ctgaaaacct ccaactctta cttaagaaaa aaggcagcac tgtgtgctgt tcatgtcatc
     1441 aggaaagttc ctgaacttat ggagatgttt ttaccagcaa caaaaaattt attgaatgag
     1501 aagaaccatg gtgtcctcca cacatctgta gtcctcctca cagaaatgtg tgagcgaagc
     1561 ccagacatgc ttgcgcattt cagaaagctt gtgccccaat tagttcgtat tttaaagaac
     1621 ctcatcatgt ccggatattc accagaacat gatgtttctg gtatcagtga cccctttttg
     1681 caggtacgaa ttttgcggtt attaagaatt ttaggacgaa atgatgatga ttcaagtgaa
     1741 gctatgaatg atatattagc acaggttgcc actaatactg agactagtaa aaatgtagga
     1801 aatgctattc tttatgaaac ggttttgact atcatggata ttaagtcaga gagtggattg
     1861 cgagtcctag ccataaatat cctgggtcgt ttcttattga acaatgacaa gaatattaga
     1921 tatgtggctc tgacatcttt gttgaagact gtacagacag atcataatgc agtacagagg
     1981 cacagaagca caattgtgga ctgtcttaaa gatttggatg tctcaataaa acggcgtgca
     2041 atggaattga gttttgccct ggtaaatggg aataatatcc gaggcatgat gaaagaatta
     2101 ctttattttc tggattcgtg tgagccagaa tttaaagcag actgtgcatc tggaatcttt
     2161 cttgctgcag aaaagtatgc accttccaaa cgatggcata tagacacaat tatgcgtgtt
     2221 ttgacaacgg caggaagtta tgttcgtgat gatgcagtcc ccaatttaat ccagttaata
     2281 actaatagtg tggagatgca tgcctatact gtccagcgcc tgtacaaagc aattcttggt
     2341 gattattctc aacaaccttt ggtacaagtg gctgcatggt gtataggtga atatggtgat
     2401 cttcttgtat ctggccagtg tgaagaggaa gagcctattc aggtaacaga ggatgaagtg
     2461 ttggatattt tagaaagtgt cctaatctct aatatgtcca cctctgtgac acgaggttat
     2521 gccctcactg ccattatgaa gctttccact cgattcactt gtactgtaaa ccgaattaag
     2581 aaagtggttt ccatctacgg aagcagcatt gatgtggaac tccagcagag ggcagtagaa
     2641 tataatgcac ttttcaagaa atatgaccac atgaggtctg ccctacttga gagaatgcct
     2701 gtcatggaaa aagtgaccac aaatggccct actgagattg tgcagacaaa tggagagaca
     2761 gaaccagctc cactagagac caaaccgcca ccctctgggc cacagcccac cagccaggcc
     2821 aatgatttat tggatttgtt gggaggaaat gacataacac ctgttattcc aactgcgcct
     2881 acaagcaaac catcttctgc tggtggagaa cttcttgatt tgctgggaga catcaacctt
     2941 acaggtgctc cagctgctgc tcctgcccct gcctcagtcc cacagatatc ccagccccac
     3001 ttcttgttgg atgggctttc atcacagcct ctcttcaatg atattgctgc aggcatcccc
     3061 tccatcacag catacagtaa gaatggcttg aagatagaat tcacctttga acggtcaaat
     3121 accaacccca gtgtaacagt gataacgata caggcctcca acagcacaga gctagatatg
     3181 acggactttg ttttccaagc tgcagtacca aagacattcc agctgcagct cttgtctcct
     3241 agcagcagca ttgtcccagc atttaacacg gggaccatca cacaagtcat taaagttctg
     3301 aaccctcaga agcaacagct gcgaatgcgg atcaagctta catataatca caagggctca
     3361 gcaatgcaag atctagcaga ggtgaacaac tttccccctc agtcctggca atgagggttt
     3421 ggcaccattc tcattcttta tcccactcaa tcaaaggaac tctgggaagg aggttgtgat
     3481 tgctggcaag tcccccccaa ctgtaccacg ggcatgagga gctgaagaga actgctgagg
     3541 aggattttcc taaagttact gctgaccttg aagcattgtt aaagactaat gtcctctcct
     3601 ccactgttga ggctggctgc ttctggaggc tactttgcac tcttcctctt ctcctttttc
     3661 cgcacttctc cacccctccc acatttacag ccagaatcaa cattccctgg gccgcactcg
     3721 agcatgcatc tagagggccg cgatccgtcg agcatgcatc tagagggccc tattctatag
     3781 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     3841 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3901 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3961 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4021 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4081 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4141 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4201 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4261 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4321 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4381 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4441 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4501 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4561 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4621 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4681 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4741 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4801 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4861 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4921 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4981 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5041 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5101 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5161 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5221 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5281 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5341 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5401 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5461 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5521 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5581 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5641 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5701 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5761 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5821 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5881 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5941 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6001 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6061 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6241 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6661 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6901 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7081 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8161 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8221 c
//
