LOCUS       sequence-27553-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, 
            Benjamin K, Gordon A, Brent R
  TITLE     Negative feedback that improves information transmission in yeast 
            signalling.
  JOURNAL   Nature. 2008 Dec 11. 456(7223):755-61.
  PUBMED    19079053
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Dec 11. 456(7223):755-61."
COMMENT     Sequence Label: pRY1001
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(127..147)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgacttcat cgttttcatt attagcttga acttttaaga tttcctctgt tctactattg
       61 gttatttgga ctttcatagc gatcgccttg tatagttcat ccatgccatg tgtaatccca
      121 gcagctgtta caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa
      181 agggcagatt gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt
      241 ggagtatttt gttgataatg gtctgctagt tgaacgcttc catcttcaat gttgtgtcta
      301 gttttgaagt tagctttgat tccattcttt tgtttgtctg cagtgatgta tacattgtgt
      361 gaaatatagt tgtattccaa tttgtgtcca agaatgtttc catcttcttt aaaatcaata
      421 ccttttaact cgattctatt aacaagggta tcaccttcaa acttgacttc agcacgtgtc
      481 ttgtagttcc cgtcatcttt gaaaaatata gttctttcct gtacataacc ttcgggcatg
      541 gcactcttga aaaagtcatg ccgtttcata tgatctgggt atcttgaaaa gcattgaaca
      601 ccccaagtta aagtagtgac aagtgttggc catggaacag gtagttttcc agtagtgcaa
      661 ataaatttaa gggtaagttt tccgtatgtt gcatcacctt caccctctcc actgacagaa
      721 aattgtgcca ttaacatcac catctaattc aacaagattg ggacaccc
//
