LOCUS       sequence-275538-        5841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP3
FEATURES             Location/Qualifiers
     source          1..5841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccccta ccaaggtgac cttcggagac gacaccgtga tcgaggtgca gggttacaag
       61 agcgtgaaca tcaccttcga gctggacgaa cgcatcgaca aggtgctgaa cgaaaagtgc
      121 tccgcttaca ccgtggagct gggcaccgag gtgaacgaat tcgcctgcgt ggtggccgac
      181 gctgtgatca agaccctgca gccagtgtcc gaactgctga cccctctggg tatcgacctg
      241 gacgagtggt ctatggctac ctactacctg ttcgacgaga gcggcgaatt caagctggcc
      301 tctcacatgt actgcagctt ctaccctccc gacgaagacg aggaagaggg tgactgcgaa
      361 gaggaagagt tcgagccttc cactcagtac gagtacggca ccgaagacga ctaccagggc
      421 aagcctctgg aattcggagc cacctctgcc gctctgcagc ctgaagagga acaggaggaa
      481 gactggctgg acgacgactc ccagcagacc gtgggtcagc aggacggctc tgaagacaac
      541 cagaccacca ccatccagac catcgtggag gtgcagcctc agctggagat ggaactgacc
      601 ccagtggtgc agaccatcga ggtgaacagc ttctccggct acctgaagct gaccgacaac
      661 gtgtacatca agaacgctga catcgtggag gaagccaaga aggtgaaacc aaccgtggtg
      721 gtgaacgccg ctaacgtgta cctgaagcac ggcggaggtg tggctggagc tctgaacaag
      781 gctaccaaca acgccatgca ggtggaatcc gacgactaca tcgctaccaa cggtcctctg
      841 aaggtgggcg gatcctgcgt gctgtctggc cacaacctgg ccaagcactg cctgcacgtg
      901 gtgggaccaa acgtgaacaa gggagaggac atccagctgc tgaagagcgc ctacgaaaac
      961 ttcaaccagc acgaggtgct gctggctcca ctgctgtctg ccggaatctt cggtgctgat
     1021 cccatccaca gcctgcgcgt gtgcgtggac accgtgagga ccaacgtgta cctggccgtg
     1081 ttcgacaaga acctgtacga caagctggtg agctccttcc tggagatgaa gtccgaaaag
     1141 caggtggagc agaagatcgc cgagatcccc aaggaggaag tgaagccttt catcaccgaa
     1201 tctaagccca gcgtggagca gagaaagcag gacgacaaga agatcaaggc ctgcgtggag
     1261 gaagtgacca ccaccctgga ggaaaccaag ttcctgaccg agaacctgct gctgtacatc
     1321 gacatcaacg gcaacctgca ccctgactcc gctaccctgg tgtctgacat cgacatcacc
     1381 ttcctgaaga aggacgcccc ctacatcgtg ggcgacgtgg tgcaggaagg agtgctgacc
     1441 gctgtggtca tccctaccaa gaaggccggt ggcaccaccg agatgctggc caaggctctg
     1501 cgtaaggtgc caaccgacaa ctacatcacc acctaccccg gtcagggcct gaacggttac
     1561 accgtggagg aagctaagac cgtgctgaag aagtgcaagt ctgccttcta catcctgccc
     1621 tccatcatct ctaacgagaa gcaggaaatc ctgggcaccg tgagctggaa cctgcgcgaa
     1681 atgctggccc acgctgagga aaccaggaag ctgatgcccg tgtgcgtgga gaccaaggcc
     1741 atcgtgagca ccatccagag aaagtacaag ggcatcaaga tccaggaggg tgtggtggac
     1801 tacggcgctc gtttctactt ctacacctcc aagaccaccg tggcctctct gatcaacacc
     1861 ctgaacgacc tgaacgagac cctggtgacc atgccactgg gatacgtgac ccacggtctg
     1921 aacctggagg aagccgctag atacatgcgt tccctgaagg tgcctgctac cgtgagcgtg
     1981 agcagccctg acgctgtgac cgcctacaac ggatacctga cctcctctag caagacccct
     2041 gaggaacact tcatcgaaac catcagcctg gccggttcct acaaggactg gtcttacagc
     2101 ggacagtcca cccagctggg catcgagttc ctgaagcgcg gagacaagag cgtgtactac
     2161 acctccaacc caaccacctt ccacctggac ggtgaagtga tcaccttcga caacctgaag
     2221 accctgctgt ctctgcgcga ggtgaggacc atcaaggtgt tcaccaccgt ggacaacatc
     2281 aacctgcaca cccaggtggt ggacatgagc atgacctacg gacagcagtt cggtcctacc
     2341 tacctggacg gagctgacgt gaccaagatc aagccacaca actcccacga gggcaagacc
     2401 ttctacgtgc tgcctaacga tgacaccctg agagtggagg ctttcgaata ctaccacacc
     2461 accgacccat ccttcctggg caggtacatg tctgccctga accacaccaa gaagtggaag
     2521 tacccccagg tgaacggact gaccagcatc aagtgggccg acaacaactg ctacctggcc
     2581 accgctctgc tgaccctgca gcagatcgaa ctgaagttca acccacccgc tctgcaggac
     2641 gcttactaca gagcccgtgc tggcgaggcc gctaacttct gcgctctgat cctggcctac
     2701 tgcaacaaga ccgtgggcga actgggagac gtgagagaga ccatgagcta cctgttccag
     2761 cacgccaacc tggactcctg caagcgtgtg ctgaacgtgg tgtgcaagac ctgcggccag
     2821 cagcagacca ccctgaaggg agtggaagct gtgatgtaca tgggtaccct gtcctacgag
     2881 cagttcaaga agggcgtgca gatcccttgc acctgcggaa agcaggccac caagtacctg
     2941 gtgcagcagg aaagcccatt cgtgatgatg tccgctcctc cagcccagta cgaactgaag
     3001 cacggcacct tcacctgcgc ttccgagtac accggcaact accagtgcgg acactacaag
     3061 cacatcacct ctaaggagac cctgtactgc atcgacggag ccctgctgac caagtcctct
     3121 gaatacaagg gtcccatcac cgacgtgttc tacaaggaga acagctacac caccaccatc
     3181 aagcccgtga cctacaagct ggacggcgtg gtgtgcaccg aaatcgaccc taagctggac
     3241 aactactaca agaaggacaa ctcttacttc accgagcagc ctatcgacct ggtgccaaac
     3301 cagccctacc ctaacgccag cttcgacaac ttcaagttcg tgtgcgacaa catcaagttc
     3361 gctgacgacc tgaaccagct gaccggatac aagaagcctg cctctagaga gctgaaggtg
     3421 accttcttcc cagacctgaa cggcgacgtg gtggctatcg actacaagca ctacacccca
     3481 agcttcaaga agggagccaa gctgctgcac aagcccatcg tgtggcacgt gaacaacgct
     3541 accaacaagg ccacctacaa gcctaacacc tggtgcatcc gttgcctgtg gtctaccaag
     3601 ccagtggaaa cctccaactc tttcgacgtg ctgaagagcg aggacgctca gggcatggac
     3661 aacctggcct gcgaagacct gaagcccgtg agcgaggaag tggtggagaa cccaaccatc
     3721 cagaaggacg tgctggaatg caacgtgaag accaccgagg tggtgggaga catcatcctg
     3781 aagccagcta acaacagcct gaagatcacc gaggaagtgg gtcacaccga cctgatggcc
     3841 gcttacgtgg acaacagctc cctgaccatc aagaagccca acgagctgag cagggtgctg
     3901 ggtctgaaga ccctggctac tcacggtctg gctgctgtga actctgtgcc atgggacacc
     3961 atcgctaact acgccaagcc tttcctgaac aaggtggtgt ccaccaccac caacatcgtg
     4021 accagatgtc tgaaccgcgt gtgcaccaac tacatgccct acttcttcac cctgctgctg
     4081 cagctgtgca ccttcaccag aagcaccaac tcccgtatca aggctagcat gcctaccacc
     4141 atcgccaaga acaccgtgaa gagcgtggga aagttctgcc tggaggcttc cttcaactac
     4201 ctgaagtctc caaacttcag caagctgatc aacatcatca tctggttcct gctgctctcc
     4261 gtgtgcctgg gttccctgat ctactctacc gccgctctgg gagtgctgat gtctaacctg
     4321 ggtatgccta gctactgcac tggttacaga gagggatacc tgaacagcac caacgtgacc
     4381 atcgccacct actgcaccgg cagcatccca tgctccgtgt gcctgtctgg actggacagc
     4441 ctggacacct acccctccct ggaaaccatc cagatcacca tctctagctt caagtgggac
     4501 ctgaccgcct tcggtctggt ggctgagtgg ttcctggcct acatcctgtt caccaggttc
     4561 ttctacgtgc tgggcctggc cgctatcatg cagctgttct tctcctactt cgctgtgcac
     4621 ttcatcagca actcctggct gatgtggctg atcatcaacc tggtgcagat ggctcccatc
     4681 tctgccatgg tgcgcatgta catcttcttc gccagcttct actacgtgtg gaagtcctac
     4741 gtgcacgtgg tggacggttg caactcctct acctgcatga tgtgctacaa gcgcaacagg
     4801 gctaccaggg tggagtgcac caccatcgtg aacggcgtgc gcaggtcctt ctacgtgtac
     4861 gccaacggcg gcaagggatt ctgcaagctg cacaactgga actgcgtgaa ctgcgacacc
     4921 ttctgcgctg gctccacctt catctctgac gaggtggcca gagacctgag cctgcagttc
     4981 aagcgtccaa tcaaccccac cgaccagagc tcctacatcg tggactctgt gaccgtgaag
     5041 aacggtagca tccacctgta cttcgacaag gctggccaga agacctacga gcgccactct
     5101 ctgagccact tcgtgaacct ggacaacctg agggccaaca acaccaaggg cagcctgcct
     5161 atcaacgtga tcgtgttcga cggaaagtcc aagtgcgagg aatctagcgc caagtccgcc
     5221 agcgtgtact acagccagct gatgtgccag ccaatcctgc tgctggacca ggctctggtg
     5281 tctgacgtgg gagacagcgc cgaagtggcc gtgaagatgt tcgacgccta cgtgaacacc
     5341 ttctcctcta ccttcaacgt gcctatggag aagctgaaga ccctggtggc tactgctgag
     5401 gctgaactgg ctaagaacgt gtccctggac aacgtgctga gcaccttcat ctccgccgct
     5461 aggcagggtt tcgtggactc tgacgtggaa accaaagacg tggtggagtg cctgaagctg
     5521 tctcaccaga gcgacatcga agtgaccggc gactcctgca acaactacat gctgacctac
     5581 aacaaggtgg agaacatgac cccaagagac ctgggagctt gcatcgactg ctccgcccgt
     5641 cacatcaacg ctcaggtggc caagtctcac aacatcgccc tgatctggaa cgtgaaggac
     5701 ttcatgagcc tgtccgagca gctgcgcaag cagatcagga gcgccgctaa gaagaacaac
     5761 ctgcccttca agctgacctg cgctaccacc agacaggtgg tgaacgtggt gaccaccaag
     5821 atcgccctga agggcggata a
//
