LOCUS       sequence-27555-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, 
            Benjamin K, Gordon A, Brent R
  TITLE     Negative feedback that improves information transmission in yeast 
            signalling.
  JOURNAL   Nature. 2008 Dec 11. 456(7223):755-61.
  PUBMED    19079053
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Dec 11. 456(7223):755-61."
COMMENT     Sequence Label: pRY1004
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(166..353)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     274..298
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(560..580)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcggccgct ctagaactag tggatccgtg tggaagaacg attacaacag gtgttgtcct
       61 ctgaggacat aaaatacaca ccgagattca tcaactcatt gctggagtta gcatatctac
      121 aattgggtga aatggggagc gatttgcagg catttgctcg gcatgccggt agaggtgtgg
      181 tcaataagag cgacctcatg ctatacctga gaaagcaacc tgacctacag gaaagagtta
      241 ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt
      301 atttttttta taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta
      361 gaagtgtcaa caacgtatct accaacgatt tgaccctttt ccatcttttc gtaaatttct
      421 ggcaaggtag acaagccgac aaccttgatt ggagacttga ccaaacctct ggcgaagaag
      481 tccaaagctc ggaattgcgt ctcgagttca ccccgaccgc ttgtatagtt catccatgcc
      541 atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt
      601 gggatctttc gaaagtttag attgatagga caggtaatgg ttgtctggta aaaggacagg
      661 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc ttccatcttc
      721 aatgttgtgt ctaattttga agttaactgt gattccattc ttttgtttgt ctgncatgat
      781 gta
//
