LOCUS       sequence-275562-        2875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with 
            RBS2, p15a origin, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM532
FEATURES             Location/Qualifiers
     source          1..2875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             160..171
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      772..858
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(893..910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1027..1572)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1107..1126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1686..1780)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1810..2601,2602..2670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2433..2452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2671..2775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2843..2861
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagcaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc
       61 tggataactt tacgggcatg cataaggctc gtataatata ttcagggaga ccacaacggt
      121 ttccctctac aaataatttt gtttaacttt gaattcatta aagaggagaa agatgccaaa
      181 atcaacctat tttagtcttt ctgacgaaaa acgaaaacgt gtctatgatg cctgtttact
      241 agaatttcaa acgcactctt tccatgaagc taaaatcatg cacatcgtaa aagcacttga
      301 tatcccaaga ggaagttttt atcaatactt tgaagatttg aaggattcat actattatat
      361 cttgtcacag gaaactgtcg agattcatga tttatttttt aatttactaa aagaatatcc
      421 tctagaagtt gctcttaata aatacaagta tcttcttctt gaaaatttag taaattcgcc
      481 ccaatataat ctttataaat atcgattttt agattggact tatgaattag aaagagattg
      541 gaagcctaaa ggcgaggtaa ctgttcccgc tcgtgaactt gataatccta tttcccaagt
      601 attaaaatca gtcattcaca atctagttta tcgcatgttt agtgaaaatt gggatgaaca
      661 aaagtttatt gaaacttacg ataaagaaat caaattgctc acagagggct tgcttaatta
      721 tgttactgaa agcaaaaaat agggatccca tggtacgcgt gctagaggca tcaaataaaa
      781 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      841 ctcctgagta ggacaaatcc gccgccctag acctagggat atattccgct tcctcgctca
      901 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
      961 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     1021 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     1081 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     1141 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     1201 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     1261 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     1321 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     1381 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     1441 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     1501 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     1561 aaacgatctc aagaagatca tcttattaat cagataaaat attactagat ttcagtgcaa
     1621 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gttactagtg
     1681 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1741 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg
     1801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2821 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcac
//
