LOCUS       sequence-275575-        4184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene tagging vector to add mCherry Tag and membrane anchoring motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Wosika V, Pelet S
  TITLE     Single-particle imaging of stress-promoters induction reveals the 
            interplay between MAPK signaling, chromatin and transcription 
            factors.
  JOURNAL   Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
  PUBMED    32576833
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-23"; volume: "11(1):3171. doi"; pages: " 
            10.1038/s41467-020-16943-w"
COMMENT     Sequence Label: pSP566
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(109..172)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        199..217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(379..397)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     647..666
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1010..1197
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1065..1089)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1272..1615
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    2541..2574
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2575..2593
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2622..2640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2673..3261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2753..2772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3432..4184,1..39,40..108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4055..4074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
       61 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
      121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaagt
      181 atcgttcgaa ttaattaaaa ttaaccctca ctaaaggggc atgcgcggcc gctctagaac
      241 taaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      301 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      361 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      421 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      481 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      541 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      601 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      661 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      721 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      781 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaatatca
      841 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      901 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatcgcta gcaggatcgg
      961 gtggctgttg tattataagt taagtcgagg gcgcgccact tctaaataag cgaatttctt
     1021 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     1081 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     1141 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
     1201 atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc ccgccgggtc
     1261 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
     1321 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
     1381 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
     1441 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
     1501 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
     1561 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatgag
     1621 tgtcataaac tttaccggat cgagtggacc tctcgtcaag gtatgcgggc tccagtccac
     1681 tgaagcagcg gagtgtgccc tagactctga tgccgatctc ttgggcatta tctgcgtccc
     1741 gaataggaaa cgcaccattg atcctgtaat cgcgcgcaag atttcttcgc tcgtaaaggc
     1801 ctacaagaac tcttcaggaa cgccgaagta tttagtcggt gtttttcgta atcagcctaa
     1861 agaggacgta ttggcactcg taaacgacta cggcatagat atagtgcagt tacatgggga
     1921 cgagagctgg caggaatatc aagagttcct gggcttgcct gtcattaaac gactggtttt
     1981 tccgaaagat tgtaatatcc ttttgtcagc agcgtcgcag aaaccgcact cattcatacc
     2041 gctcttcgac tcggaagcag gcggtacagg ggaactactg gactggaata gtataagtga
     2101 ctgggtcgga cggcaagaga gtccggagtc cttacatttt atgctggccg ggggtttaac
     2161 ccccgaaaac gtgggagatg ccctccgatt aaatggggtt atcggtgtcg atgtatcagg
     2221 tggggtggag acgaatggcg tgaaggactc aaataaaatc gctaattttg tgaaaaacgc
     2281 taagaaatga gaatattgat aaatttaaat tttggaatga gggttgtcct ttcctttaca
     2341 atagaggcat ttgagtgagt aggttttatc accttcttgc tcttattaaa tcgcagttgt
     2401 ctttccggta tgtgcttgac tgaggaacaa tattttattc atgatacatt tccatgctac
     2461 gcatatcgtc atccacatgg aaggtaattt tcttctgtgg cctttcatat agttgtgaat
     2521 ggtagaacaa tcttcatata ataacttcgt ataatgtatg ctatacgaag ttattaatac
     2581 gactcactat agggcgaatt ggagctccaa ttcggacgtc ccctatagtg agtcgtatta
     2641 ttaattaatt cgaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3361 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actg
//
