LOCUS       sequence-275585-       11351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in
            S. cerevisiae. Expresses both Cas9 and STL1 sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Wosika V, Pelet S
  TITLE     Single-particle imaging of stress-promoters induction reveals the 
            interplay between MAPK signaling, chromatin and transcription 
            factors.
  JOURNAL   Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
  PUBMED    32576833
REFERENCE   2  (bases 1 to 11351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-23"; volume: "11(1):3171. doi"; pages: " 
            10.1038/s41467-020-16943-w"
COMMENT     Sequence Label: pSP571
FEATURES             Location/Qualifiers
     source          1..11351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             505..1167
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1427..1882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1776..1795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2049..2068
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2049..2067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2092..2108
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2093..2109
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2154..2820
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2690..2713
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      2849..2858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2855..4573
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6968..6988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7016..7203
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7071..7095)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(7321..7340)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(7344..7419)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7446..7714)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(7745..7765)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7745..7763)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7784..7800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7784..7800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7797..7819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7808..7824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7832..7838,7839..7856,7857..7862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7877..7898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8015..8032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8186..8774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8266..8285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8945..9736,9737..9805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9568..9587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9806..9910)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9937..11283
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     11324..11342
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
      421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
      481 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
      541 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
      601 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
      661 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
      721 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
      781 actgaagact gcgggattgc tctcggtcaa gcatttaaag aggccctagg ggccgtgcgt
      841 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
      901 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
      961 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gttttgcaga ggctagcaga
     1021 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
     1081 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac aaacgatgtt
     1141 ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagttgc agcatacgat
     1201 atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta
     1261 tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg
     1321 ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgacg gatctatgcg
     1381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     1441 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1861 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
     2101 acggtatcga taagcttgat atcgaattca gtctactagt cagttcgagt ttatcattat
     2161 caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc ctaactttat
     2221 ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt acatgcccaa aatagggggc
     2281 gggttacaca gaatatataa catcgtaggt gtctgggtga acagtttatt cctggcatcc
     2341 actaaatata atggagcccg ctttttaagc tggcatccag aaaaaaaaag aatcccagca
     2401 ccaaaatatt gttttcttca ccaaccatca gttcataggt ccattctctt agcgcaacta
     2461 cagagaacag gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc
     2521 tgcctggagt aaatgatgac acaaggcaat tgacccacgc atgtatctat ctcattttct
     2581 tacaccttct attaccttct gctctctctg atttggaaaa agctgaaaaa aaaggttgaa
     2641 accagttccc tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt
     2701 gattgtaatt ctgtaaatct atttcttaaa cttcttaaat tctactttta tagttagtct
     2761 tttttttagt tttaaaacac caagaactta gtttcgaata aacacacata aacaaacaaa
     2821 agatctgata tggatcgaat tagatctcgc caccatggac aagaagtatt ctatcggact
     2881 ggacatcggg actaatagcg tcgggtgggc cgtcatcact gacgagtaca aggtgccctc
     2941 taagaagttc aaggtgctcg ggaacaccga ccggcattcc atcaagaaaa atctgatcgg
     3001 agctctcctc tttgattcag gggagaccgc tgaagcaacc cgcctcaagc ggactgctag
     3061 acggcggtac accaggagga agaaccggat ttgttacctt caagagatat tctccaacga
     3121 aatggcaaag gtcgacgaca gcttcttcca taggctggaa gaatcattcc tcgtggaaga
     3181 ggataagaag catgaacggc atcccatctt cggtaatatc gtcgacgagg tggcctatca
     3241 cgagaaatac ccaaccatct accatcttcg caaaaagctg gtggactcaa ccgacaaggc
     3301 agacctccgg cttatctacc tggccctggc ccacatgatt aagttcagag gccacttcct
     3361 gatcgagggc gacctcaatc ctgacaatag cgatgtggat aaactgttca tccagctggt
     3421 gcagacttac aaccagctct ttgaagagaa ccccatcaat gcaagcggag tcgatgccaa
     3481 ggccattctg tcagcccggc tgtcaaagag ccgcagactt gagaatctta tcgctcagct
     3541 gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt gctctttcac ttgggctgac
     3601 tcccaatttc aagtctaatt tcgacctggc agaggatgcc aagctgcaac tgtccaagga
     3661 cacctatgat gacgatctcg acaacctcct ggcccagatc ggtgaccaat acgccgacct
     3721 tttccttgct gctaagaatc tttctgacgc catcctgctg tctgacattc tccgcgtgaa
     3781 cactgaaatc accaaggccc ctctttcagc ttcaatgatt aagcggtatg atgagcacca
     3841 ccaggacctg accctgctta aggcactcgt ccggcagcag cttccggaga agtacaagga
     3901 aatcttcttt gaccagtcaa agaatggata cgccggctac atcgacggag gtgcctccca
     3961 agaggaattt tataagttta tcaaacctat ccttgagaag atggacggca ccgaagagct
     4021 cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag cgcactttcg acaatgggag
     4081 cattccccac cagatccatc ttggggagct tcacgccatc cttcggcgcc aagaggactt
     4141 ctaccccttt cttaaggaca acagggagaa gattgagaaa attctcactt tccgcatccc
     4201 ctactacgtg ggacccctcg ccagaggaaa tagccggttt gcttggatga ccagaaagtc
     4261 agaagaaact atcactccct ggaacttcga agaggtggtg gacaagggag ccagcgctca
     4321 gtcattcatc gaacggatga ctaacttcga taagaacctc cccaatgaga aggtcctgcc
     4381 gaaacattcc ctgctctacg agtactttac cgtgtacaac gagctgacca aggtgaaata
     4441 tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt
     4501 ggaccttctg ttcaagacca atagaaaggt gaccgtgaag cagctgaagg aggactattt
     4561 caagaaaatt gaatgcttcg actctgtgga gattagcggg gtcgaagatc ggttcaacgc
     4621 aagcctgggt acctaccatg atctgcttaa gatcatcaag gacaaggatt ttctggacaa
     4681 tgaggagaaa gaggacatcc ttgaggacat tgtcctgact ctcactctgt tcgaggaccg
     4741 ggaaatgatc gaggagaggc ttaagaccta cgcccatctg ttcgacgata aagtgatgaa
     4801 gcaacttaaa cggagaagat ataccggatg gggacgcctt agccgcaaac tcatcaacgg
     4861 aatccgggac aaacagagcg gaaagaccat tcttgatttc cttaagagcg acggattcgc
     4921 taatcgcaac ttcatgcaac ttatccatga tgattccctg acctttaagg aggacatcca
     4981 gaaggcccaa gtgtctggac aaggtgactc actgcacgag catatcgcaa atctggctgg
     5041 ttcacccgct attaagaagg gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa
     5101 ggtgatgggt cgccataaac cagagaacat tgtcatcgag atggccaggg aaaaccagac
     5161 tacccagaag ggacagaaga acagcaggga gcggatgaaa agaattgagg aagggattaa
     5221 ggagctcggg tcacagatcc ttaaagagca cccggtggaa aacacccagc ttcagaatga
     5281 gaagctctat ctgtactacc ttcaaaatgg acgcgatatg tatgtggacc aagagcttga
     5341 tatcaacagg ctctcagact acgacgtgga ccacatcgtc cctcagagct tcctcaaaga
     5401 cgactcaatt gacaataagg tgctgactcg ctcagacaag aaccggggaa agtcagataa
     5461 cgtgccctca gaggaagtcg tgaaaaagat gaagaactat tggcgccagc ttctgaacgc
     5521 aaagctaatc actcagcgga agttcgacaa tctcactaag gctgagaggg gcggactgag
     5581 cgaactggac aaagcaggat tcattaaacg gcaacttgtg gagactcggc agattactaa
     5641 acatgtagcc caaatccttg actcacgcat gaataccaag tacgacgaaa acgacaaact
     5701 tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg gtcagcgatt tcagaaagga
     5761 ctttcaattc tacaaagtgc gggagatcaa taactatcat catgctcatg acgcatatct
     5821 gaatgccgtg gtgggaaccg ccctaatcaa gaagtaccca aagctggaaa gcgagttcgt
     5881 gtacggagac tacaaggtct acgacgtgcg caagatgatt gccaaatctg agcaggagat
     5941 cggaaaggcc accgcaaagt acttcttcta cagcaacatc atgaatttct tcaagaccga
     6001 aatcaccctt gcaaacggtg agatccggaa gaggccgctc atcgagacta atggggagac
     6061 tggcgaaatc gtgtgggaca agggcagaga tttcgctacc gtgcgcaaag tgctttctat
     6121 gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc ggaggctttt ctaaggaatc
     6181 aatcctcccc aagcgcaact ccgacaagct cattgcaagg aagaaggatt gggaccctaa
     6241 gaagtacggc ggattcgatt caccaactgt ggcttattct gtcctggtcg tggctaaggt
     6301 ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa ctgctgggta tcaccattat
     6361 ggagcgcagc tccttcgaga agaacccaat tgactttctc gaagccaaag gttacaagga
     6421 agtcaagaag gaccttatca tcaagctccc aaagtatagc ctgttcgaac tggagaatgg
     6481 gcggaagcgg atgctcgcct ccgctggcga acttcagaag ggtaatgagc tggctctccc
     6541 ctccaagtac gtgaatttcc tctaccttgc aagccattac gagaagctga aggggagccc
     6601 cgaggacaac gagcaaaagc aactgtttgt ggagcagcat aagcattatc tggacgagat
     6661 cattgagcag atttccgagt tttctaaacg cgtcattctc gctgatgcca acctcgataa
     6721 agtccttagc gcatacaata agcacagaga caaaccaatt cgggagcagg ctgagaatat
     6781 catccacctg ttcaccctca ccaatcttgg tgcccctgcc gcattcaagt acttcgacac
     6841 caccatcgac cggaaacgct atacctccac caaagaagtg ctggacgcca ccctcatcca
     6901 ccagagcatc accggacttt acgaaactcg gattgacctc tcacagctcg gaggggatga
     6961 gggagctccc aagaaaaagc gcaaggtagg tagttccgga tcctagactc tcgaggcgaa
     7021 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     7081 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     7141 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac
     7201 cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc tctaaggcgg ccgtggtatc
     7261 gtttagattg gcaattacag tgtcttagct cacatgctta taactaatta catgactcga
     7321 agacataaaa aacaaaaaaa gcagcaccga ctcggtgcca ctttttcaag ttgataacgg
     7381 actagcctta ttttaacttg ctatttctag ctctaaaacc agtgactggt tctgcttata
     7441 cgtctgatca tttatctttc actgcggaga agtttcgaac gccgaaacat gcgcaccaac
     7501 tttcacttct acagcgtttg accaaaatct tttgaacaga acattgtagg gtgtgaaaaa
     7561 atgcgcacct ttaccgctag cccaagaggg cactacaaaa tctaaggttg tacttcaaac
     7621 gtacatgtaa tcaccttgta tatactcgaa agaaaacatc aagtttctgt ataaatatga
     7681 gtgaaagcat aatcatacat tatcttttca aagattgtcc gcggtggagc tccagctttt
     7741 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     7801 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7861 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7921 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7981 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8821 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8881 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8941 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9001 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9061 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9121 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9181 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9241 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9301 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9361 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9421 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9481 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9541 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9601 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9661 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9721 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9781 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9841 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9901 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     9961 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    10021 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
    10081 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
    10141 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
    10201 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
    10261 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
    10321 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
    10381 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
    10441 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
    10501 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    10561 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    10621 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    10681 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    10741 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    10801 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    10861 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc
    10921 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
    10981 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
    11041 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg
    11101 tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat
    11161 cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg
    11221 ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg
    11281 atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    11341 gccctttcgt c
//
