LOCUS       sequence-275601-        6726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for the  PP7-mCherry protein in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Wosika V, Pelet S
  TITLE     Single-particle imaging of stress-promoters induction reveals the 
            interplay between MAPK signaling, chromatin and transcription 
            factors.
  JOURNAL   Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
  PUBMED    32576833
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-23"; volume: "11(1):3171. doi"; pages: " 
            10.1038/s41467-020-16943-w"
COMMENT     Sequence Label: pVW296
FEATURES             Location/Qualifiers
     source          1..6726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     251..268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    314..347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        384..727
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1546..1743
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1767..1800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1801..1820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1801..1819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2584..3288
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3722..4423
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(3861..3879)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4129..4148
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4439..4686
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4455..4473)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(4709..4727)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(5015..5603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5095..5114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5774..6565,6566..6634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6397..6416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6635..6698)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgatgatatt tcataaataa tgtaattcta tatatgttaa ttaccttttt tgcgaggcat
       61 atttatggtg aaggataagt tttgaccatc aaagaaggtt aatgtggctg tggtttcagg
      121 gtccataatg cttttcaatt catctttttt ttttttgttc ttttttttga ttccggtttc
      181 tttgaaattt ttttgattcg gtaatctccg agcagaagga agaacgaagg aaggagcaca
      241 gacttagatt tgtaaaacga cggccagtgc atgcgtatcg gacgtctccc ttcatggtca
      301 tagctgtttc ctgataactt cgtataatgt atgctatacg aagttattag cttgcctcgt
      361 ccccgccggg tcacccggcg agcgacatgg aggcccagaa taccctcctt gacagtcttg
      421 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      481 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      541 gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      601 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      661 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      721 aacaaccatg acagtcaaca ctaataccta tagtgagaga gcagaaactc atgcctcacc
      781 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
      841 tgacgttgat accactaagg agttccttga attaattgat aaattaggtc cttatgtatg
      901 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
      961 attattagaa ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga
     1021 tattggtaat accgtaaaga aacaatatat tggtggagtt tataaaatta gtagttgggc
     1081 agatattacc aatgctcatg gtgtcactgg gaatggagtg gttgaaggat taaaacaggg
     1141 agctaaagaa accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc
     1201 atcagtggga tcattagcat acggagaata ttctcaaaaa actgttgaaa ttgctaaatc
     1261 cgataaggaa tttgttattg gatttattgc ccaacgtgat atgggtggcc aagaagaagg
     1321 atttgattgg cttattatga cacctggagt tggattagat gataaaggtg atggattagg
     1381 acaacaatat agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg
     1441 tagaggattg tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagaaatgc
     1501 tggttggaat gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag
     1561 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     1621 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     1681 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     1741 ctgctgtcga ttcgatacta acgccgataa cttcgtataa tgtatgctat acgaagttat
     1801 taatacgact cactataggg cgaattggag ctcgccggga tcgaagaaat gatggtaaat
     1861 gaaataggaa atcaaggagc atgaaggcaa aagacaaata taagggtcga acgaaaaata
     1921 aagtgaaaag tgttgatatg atgtatttgg ctttgcggcg ccgaaaaaac gagtttacgc
     1981 aattgcacaa tcatgctgac tctgtggcgg acccgcgctc ttgccggccc ggcgataacg
     2041 ctgggcgtga ggctgtgccc ggcggagttt tttgcgcctg cattttccaa ggtttaccct
     2101 gcgctaaggg gcgagattgg agaagcaata agaatgccgg ttggggttgc gatgatgacg
     2161 accacgacaa ctggtgtcat tatttaagtt gccgaaagaa cctgagtgca tttgcaacat
     2221 gagtatacta gaagaatgag ccaagacttg cgagacgcga gtttgccggt ggtgcgaaca
     2281 atagagcgac catgaccttg aaggtgagac gcgcataacc gctagagtac tttgaagagg
     2341 aaacagcaat agggttgcta ccagtataaa tagacaggta catacaacac tggaaatggt
     2401 tgtctgtttg agtacgcttt caattcattt gggtgtgcac tttattatgt tacaatatgg
     2461 aagggaactt tacacttctc ctatgcacat atattaatta aagtccaatg ctagtagaga
     2521 aggggggtaa cacccctccg cgctcttttc cgattttttt ctaaaccgtg gaatatttcg
     2581 gatatccttt tgttgtttcc gggtgtacaa tatggacttc ctcttttctg gcaaccaaac
     2641 ccatacatcg ggattcctat aataccttcg ttggtctccc taacatgtag gtggcggagg
     2701 ggagatatac aatagaacag ataccagaca agacataatg ggctaaacaa gactacacca
     2761 attacactgc ctcattgatg gtggtacata acgaactaat actgtagccc tagacttgat
     2821 agccatcatc atatcgaagt ttcactaccc tttttccatt tgccatctat tgaagtaata
     2881 ataggcgcat gcaacttctt ttcttttttt ttcttttctc tctcccccgt tgttgtctca
     2941 ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt aacgacaaag
     3001 acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa
     3061 ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat acggccttcc
     3121 ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca agtataaata
     3181 gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt tcttccttgt
     3241 ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc aagctggccg
     3301 ctctagaatg tccaaaacca tcgttctttc ggtcggcgag gctactcgca ctctgactga
     3361 gatccagtcc accgcagacc gtcagatatt tgaagagaag gtcgggcctc tggtgggtcg
     3421 gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag accgcgtatc gcgtcaacct
     3481 aaaactggat caggcggacg tggttgatac tagtcttccg aaagtgcgct acactcaggt
     3541 atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag gcctcgcgca aatcgttgta
     3601 cgatttgacc aagtccctcg tcgcgacctc gcaggtcgag gatcttgtcg tcaaccttgt
     3661 gccgctgggc cgtggttccc ctgggctgca ggagttcgac atcaagctta tgagatctgc
     3721 tagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3781 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3841 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3901 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3961 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4021 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4081 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4141 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4201 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4261 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4321 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4381 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggatcct aactcgagtc
     4441 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
     4501 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
     4561 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac
     4621 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta
     4681 atttgcggcc ggtacccagc ttttgttccc tttagtgagg gttaatttcc tgaaaactgt
     4741 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
     4801 gttattacgc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa aaaaaattgg
     4861 aaagaaaatg cttcatggcc tttataaaaa ggaactatcc aatacctcgc cagaaccaag
     4921 taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa agatggacgc
     4981 atttaattaa ttcgaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaagtatcg ttcgaattaa
     6721 ttaacg
//
