LOCUS       sequence-275621-        5662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for the  PP7-GFP protein in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Wosika V, Pelet S
  TITLE     Single-particle imaging of stress-promoters induction reveals the 
            interplay between MAPK signaling, chromatin and transcription 
            factors.
  JOURNAL   Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
  PUBMED    32576833
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-23"; volume: "11(1):3171. doi"; pages: " 
            10.1038/s41467-020-16943-w"
COMMENT     Sequence Label: pVW300
FEATURES             Location/Qualifiers
     source          1..5662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     251..268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    314..347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        384..727
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1546..1743
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1767..1800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1801..1820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1801..1819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1833..2231
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      3375..3622
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3391..3409)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(3645..3663)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(3951..4539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4031..4050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4710..5501,5502..5570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5333..5352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5571..5634)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgatgatatt tcataaataa tgtaattcta tatatgttaa ttaccttttt tgcgaggcat
       61 atttatggtg aaggataagt tttgaccatc aaagaaggtt aatgtggctg tggtttcagg
      121 gtccataatg cttttcaatt catctttttt ttttttgttc ttttttttga ttccggtttc
      181 tttgaaattt ttttgattcg gtaatctccg agcagaagga agaacgaagg aaggagcaca
      241 gacttagatt tgtaaaacga cggccagtgc atgcgtatcg gacgtctccc ttcatggtca
      301 tagctgtttc ctgataactt cgtataatgt atgctatacg aagttattag cttgcctcgt
      361 ccccgccggg tcacccggcg agcgacatgg aggcccagaa taccctcctt gacagtcttg
      421 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      481 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      541 gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      601 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      661 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      721 aacaaccatg acagtcaaca ctaataccta tagtgagaga gcagaaactc atgcctcacc
      781 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
      841 tgacgttgat accactaagg agttccttga attaattgat aaattaggtc cttatgtatg
      901 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
      961 attattagaa ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga
     1021 tattggtaat accgtaaaga aacaatatat tggtggagtt tataaaatta gtagttgggc
     1081 agatattacc aatgctcatg gtgtcactgg gaatggagtg gttgaaggat taaaacaggg
     1141 agctaaagaa accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc
     1201 atcagtggga tcattagcat acggagaata ttctcaaaaa actgttgaaa ttgctaaatc
     1261 cgataaggaa tttgttattg gatttattgc ccaacgtgat atgggtggcc aagaagaagg
     1321 atttgattgg cttattatga cacctggagt tggattagat gataaaggtg atggattagg
     1381 acaacaatat agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg
     1441 tagaggattg tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagaaatgc
     1501 tggttggaat gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag
     1561 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     1621 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     1681 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     1741 ctgctgtcga ttcgatacta acgccgataa cttcgtataa tgtatgctat acgaagttat
     1801 taatacgact cactataggg cgaattggag ctcatagctt caaaatgttt ctactccttt
     1861 tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa aacacccaag
     1921 cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta cccgtactaa
     1981 aggtttggaa aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata
     2041 aaaattttta tcacgtttct ttttcttgaa aatttttttt ttgatttttt tctctttcga
     2101 tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt ttcagtttca
     2161 tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa
     2221 tctaatctaa gttttctaga atgtccaaaa ccatcgttct ttcggtcggc gaggctactc
     2281 gcactctgac tgagatccag tccaccgcag accgtcagat atttgaagag aaggtcgggc
     2341 ctctggtggg tcggctgcgc ctcacggctt cgctccgtca aaacggagcc aagaccgcgt
     2401 atcgcgtcaa cctaaaactg gatcaggcgg acgtggttga tactagtctt ccgaaagtgc
     2461 gctacactca ggtatggtcg cacgacgtga caatcgttgc gaatagcacc gaggcctcgc
     2521 gcaaatcgtt gtacgatttg accaagtccc tcgtcgcgac ctcgcaggtc gaggatcttg
     2581 tcgtcaacct tgtgccgctg ggccgtggtt cccctgggct gcaggagttc gacatcaagc
     2641 ttatgtctaa aggcgaggaa ttgtttacag gtgtagttcc tattttggtc gaactagatg
     2701 gagatgtcaa cggtcataag ttttctgtac gtggtgaagg cgaaggtgat gctactaatg
     2761 gcaaactgac tctgaagttc atctgtacga ccggaaaatt gccagttcca tggcctacat
     2821 tagtaacaac attatgttac ggagttcaat gctttgctag atacccagat cacatgaagc
     2881 agcatgactt tttcaaaagt gctatgccag aaggttatgt gcaggagaga actatctcct
     2941 tcaaagatga tggtacttat aagacaagag cagaggtaaa gtttgaaggc gatacattgg
     3001 ttaatagaat tgagcttaaa ggcattgatt ttaaggaaga tgggaatatc ctcggacata
     3061 aacttgaata caatttcaat tcacataatg tttatatcac agcagataaa caaaagaatg
     3121 gtataaaagc gaactttaag acaagacata acgtggaaga tggtagcgtc caattagccg
     3181 accattacca acaaaacact cctatcgggg acgggccagt tctccttcca gacaaccact
     3241 acctatcaac ccaatctgtt ctatctaaag accctaatga aaaacgagat cacatggtct
     3301 tgttagagtt cgtgactgcc gctggtataa cattaggaat ggacgaattg tacaaataag
     3361 gatcctaact cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
     3421 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     3481 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     3541 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
     3601 acgctcgaag gctttaattt gcggccggta cccagctttt gttcccttta gtgagggtta
     3661 atttcctgaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
     3721 caatttaatt atatcagtta ttacgcggga atctcggtcg taatgatttc tataatgacg
     3781 aaaaaaaaaa aattggaaag aaaatgcttc atggccttta taaaaaggaa ctatccaata
     3841 cctcgccaga accaagtaac agtattttac ggggcacaaa tcaagaacaa taagacagga
     3901 ctgtaaagat ggacgcattt aattaattcg aaggccgcgt tgctggcgtt tttccatagg
     3961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5641 gtatcgttcg aattaattaa cg
//
