LOCUS       sequence-275626-        6729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for the  PP7-GFP protein in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Wosika V, Pelet S
  TITLE     Single-particle imaging of stress-promoters induction reveals the 
            interplay between MAPK signaling, chromatin and transcription 
            factors.
  JOURNAL   Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
  PUBMED    32576833
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-23"; volume: "11(1):3171. doi"; pages: " 
            10.1038/s41467-020-16943-w"
COMMENT     Sequence Label: pVW284
FEATURES             Location/Qualifiers
     source          1..6729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     328..345
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     329..345
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(374..390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(374..390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    391..424
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        461..804
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1623..1820
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1844..1877
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1878..1897
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1878..1896
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2661..3365
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      4519..4766
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4535..4553)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(4789..4807)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(5095..5683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5175..5194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5854..6645,6646..6714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6477..6496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6715..6729,1..49))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaagtatc
       61 gttcgaatta attaacgtga tgatatttca taaataatgt aattctatat atgttaatta
      121 ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa gaaggttaat
      181 gtggctgtgg tttcagggtc cataatgctt ttcaattcat cttttttttt tttgttcttt
      241 tttttgattc cggtttcttt gaaatttttt tgattcggta atctccgagc agaaggaaga
      301 acgaaggaag gagcacagac ttagatttgt aaaacgacgg ccagtgcatg cgtatcggac
      361 gtctcccttc atggtcatag ctgtttcctg ataacttcgt ataatgtatg ctatacgaag
      421 ttattagctt gcctcgtccc cgccgggtca cccggcgagc gacatggagg cccagaatac
      481 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      541 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      601 gcgaagcaaa aattacggct cctcgctcca gacctgcgag cagggaaacg ctcccctcac
      661 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
      721 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
      781 catcacatcc gaacataaac aaccatgaca gtcaacacta atacctatag tgagagagca
      841 gaaactcatg cctcaccagt agcacagcga ttatttcgat taatggaact gaagaaaacc
      901 aatttatgtg catcaattga cgttgatacc actaaggagt tccttgaatt aattgataaa
      961 ttaggtcctt atgtatgctt aatcaagact catattgata taatcaatga tttttcctat
     1021 gaatccacta ttgaaccatt attagaactt tcacgtaaac atcaatttat gatttttgaa
     1081 gatagaaaat ttgctgatat tggtaatacc gtaaagaaac aatatattgg tggagtttat
     1141 aaaattagta gttgggcaga tattaccaat gctcatggtg tcactgggaa tggagtggtt
     1201 gaaggattaa aacagggagc taaagaaacc accaccaacc aagagccaag agggttattg
     1261 atgttagctg aattatcatc agtgggatca ttagcatacg gagaatattc tcaaaaaact
     1321 gttgaaattg ctaaatccga taaggaattt gttattggat ttattgccca acgtgatatg
     1381 ggtggccaag aagaaggatt tgattggctt attatgacac ctggagttgg attagatgat
     1441 aaaggtgatg gattaggaca acaatataga actgttgatg aagttgttag cactggaact
     1501 gatattatca ttgttggtag aggattgttt ggtaaaggaa gagatccaga tattgaaggt
     1561 aaaaggtata gaaatgctgg ttggaatgct tatttgaaaa agactggcca attataatca
     1621 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     1681 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     1741 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     1801 gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgataactt cgtataatgt
     1861 atgctatacg aagttattaa tacgactcac tatagggcga attggagctc gccgggatcg
     1921 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa
     1981 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg
     2041 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg
     2101 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat
     2161 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg
     2221 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct
     2281 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt
     2341 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct
     2401 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat
     2461 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt
     2521 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag
     2581 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta
     2641 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc
     2701 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
     2761 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
     2821 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
     2881 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     2941 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     3001 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga
     3061 aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta
     3121 tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca
     3181 acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt
     3241 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     3301 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     3361 caactccaag ctggccgctc tagaatgtcc aaaaccatcg ttctttcggt cggcgaggct
     3421 actcgcactc tgactgagat ccagtccacc gcagaccgtc agatatttga agagaaggtc
     3481 gggcctctgg tgggtcggct gcgcctcacg gcttcgctcc gtcaaaacgg agccaagacc
     3541 gcgtatcgcg tcaacctaaa actggatcag gcggacgtgg ttgatactag tcttccgaaa
     3601 gtgcgctaca ctcaggtatg gtcgcacgac gtgacaatcg ttgcgaatag caccgaggcc
     3661 tcgcgcaaat cgttgtacga tttgaccaag tccctcgtcg cgacctcgca ggtcgaggat
     3721 cttgtcgtca accttgtgcc gctgggccgt ggttcccctg ggctgcagga gttcgacatc
     3781 aagcttatgt ctaaaggcga ggaattgttt acaggtgtag ttcctatttt ggtcgaacta
     3841 gatggagatg tcaacggtca taagttttct gtacgtggtg aaggcgaagg tgatgctact
     3901 aatggcaaac tgactctgaa gttcatctgt acgaccggaa aattgccagt tccatggcct
     3961 acattagtaa caacattatg ttacggagtt caatgctttg ctagataccc agatcacatg
     4021 aagcagcatg actttttcaa aagtgctatg ccagaaggtt atgtgcagga gagaactatc
     4081 tccttcaaag atgatggtac ttataagaca agagcagagg taaagtttga aggcgataca
     4141 ttggttaata gaattgagct taaaggcatt gattttaagg aagatgggaa tatcctcgga
     4201 cataaacttg aatacaattt caattcacat aatgtttata tcacagcaga taaacaaaag
     4261 aatggtataa aagcgaactt taagacaaga cataacgtgg aagatggtag cgtccaatta
     4321 gccgaccatt accaacaaaa cactcctatc ggggacgggc cagttctcct tccagacaac
     4381 cactacctat caacccaatc tgttctatct aaagacccta atgaaaaacg agatcacatg
     4441 gtcttgttag agttcgtgac tgccgctggt ataacattag gaatggacga attgtacaaa
     4501 taaggatcct aactcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc
     4561 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     4621 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     4681 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     4741 tgggacgctc gaaggcttta atttgcggcc ggtacccagc ttttgttccc tttagtgagg
     4801 gttaatttcc tgaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     4861 gcttcaattt aattatatca gttattacgc gggaatctcg gtcgtaatga tttctataat
     4921 gacgaaaaaa aaaaaattgg aaagaaaatg cttcatggcc tttataaaaa ggaactatcc
     4981 aatacctcgc cagaaccaag taacagtatt ttacggggca caaatcaaga acaataagac
     5041 aggactgtaa agatggacgc atttaattaa ttcgaaggcc gcgttgctgg cgtttttcca
     5101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6721 cctttttca
//
