LOCUS       sequence-275651-       11162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible lentiviral expression of human CLIMP63.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann 
            JA, Corn JE
  TITLE     A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial 
            Metabolism and ER-Resident UFMylation.
  JOURNAL   Cell. 2020 Mar 19;180(6):1160-1177.e20. doi: 
            10.1016/j.cell.2020.02.017. Epub 2020 Mar 10.
  PUBMED    32160526
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.02.017"; journalName: "Cell"; date: 
            "2020-03-19- 19"; volume: "180"; issue: "6"; pages: "1160-1177.e20"
COMMENT     Sequence Label: TetOn-CLIMP63-mCherry-eGFP
FEATURES             Location/Qualifiers
     source          1..11162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..82)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      876..1464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1365..1384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1618..1635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1752..1773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1788..1793,1794..1811,1812..1818)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1826..1842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1831..1853
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1850..1866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1850..1866
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1885..1905
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1887..1905
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1933..2159
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2160..2340
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2387..2512
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3005..3238
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3423..3467
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3680..3797
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3851..4165
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3859..3877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3895..3913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3930..3948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3966..3984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4002..4020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4037..4055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4073..4091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4138..4162
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6018..6725
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6163..6181)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6431..6450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    6753..6777
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6786..6795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6792..6794,6795..6797,6798..7511)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6792..6794,6795..6797,6798..7508)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6837..6858)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7098..7117)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7445..7466
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        7539..8049
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7968..7986
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8059..8850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8113..8132)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8723..8742
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             8860..8913
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8914..9531,9532..9537,9538..9573,9574..9576,9577..9612,
                     9613..9615,9616..9651,9652..9660)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9700..9720)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10118..10129)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10334..10567
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10639..10760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10676..10695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10730..10749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10800..10935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10935..11006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11007..11075,11076..11162,1..705)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
       61 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      121 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      181 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      241 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      301 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      361 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      421 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      481 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      541 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      601 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      661 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
      721 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      781 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      841 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      901 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      961 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1021 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1081 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1141 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1201 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1261 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1321 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1381 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1441 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1501 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1561 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1621 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1681 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     1741 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     1801 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1861 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     1921 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     1981 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     2041 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     2101 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     2161 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2221 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2281 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2341 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     2401 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     2461 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     2521 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     2581 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     2641 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     2701 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     2761 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     2821 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     2881 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     2941 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3001 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3061 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     3121 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     3181 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     3241 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     3301 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     3361 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     3421 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     3481 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     3541 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     3601 cattatcgtt tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa
     3661 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     3721 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     3781 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcgaa
     3841 tcgatatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     3901 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     3961 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     4021 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     4081 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     4141 tcgtttagtg aaccgtcaga tcgcctggag aattggctag cgccaccatg ccctcggcca
     4201 aacaaagggg ctccaagggc ggccacggcg ccgcgagccc ctcggagaag ggtgcccacc
     4261 cgtcgggcgg cgcggatgac gtggcgaaga agccgccgcc ggcgccgcag cagccgccgc
     4321 cgccgcccgc gccgcacccg cagcagcacc cgcagcagca cccgcagaac caggcgcacg
     4381 gcaagggcgg ccaccgcggc ggcggcggcg gcggcggcaa gtcctcctcc tcctcctccg
     4441 cctccgccgc cgctgccgcc gccgccgcct cgtcctcggc gtcctgctcg cgcaggctcg
     4501 gcagggcgct caactttctc ttctacctcg ccctggtggc ggcggccgct ttctcgggct
     4561 ggtgcgtcca ccacgtcctg gaggaggtcc agcaggtccg gcgcagccac caggacttct
     4621 cccggcagag ggaggagctg ggccagggct tgcagggcgt cgagcagaag gtgcagtctt
     4681 tgcaagccac atttggaact tttgagtcca tcttgagaag ctcccaacat aaacaagacc
     4741 tcacagagaa agctgtgaag caaggggaga gtgaggtcag ccggatcagc gaagtgctgc
     4801 agaaactcca gaatgagatt ctcaaagacc tctcggatgg gatccatgtg gtgaaggacg
     4861 cccgggagcg ggacttcacg tccctggaga acacggtgga ggagcggctg acggagctca
     4921 ccaaatccat caacgacaac atcgccatct tcacagaagt ccagaagagg agccagaagg
     4981 agatcaatga catgaaggca aaggttgcct ccctggaaga atctgagggg aacaagcagg
     5041 atttgaaagc cttaaaggaa gctgtgaagg agatacagac ctcagccaag tccagagagt
     5101 gggacatgga ggccctgaga agtacccttc agactatgga gtctgacatc tacaccgagg
     5161 tccgcgagct ggtgagcctc aagcaggagc agcaggcttt caaggaggcg gccgacacgg
     5221 agcggctcgc cctgcaggcc ctcacggaga agcttctcag gtctgaggag tccgtctccc
     5281 gcctcccgga ggagatccgg agactggagg aagagctccg ccagctgaag tccgattccc
     5341 acgggccgaa ggaggacgga ggcttcagac actcggaagc ctttgaggca ctccagcaaa
     5401 agagtcaggg actggactcc aggctccagc acgtggagga tggggtgctc tccatgcagg
     5461 tggcttctgc gcgccagacc gagagcctgg agtccctcct gtccaagagc caggagcacg
     5521 agcagcgcct ggccgccctg caggggcgcc tggaaggcct cgggtcctca gaggcagacc
     5581 aggatggcct ggccagcacg gtgaggagcc tgggcgagac ccagctggtg ctctacggtg
     5641 acgtggagga gctgaagagg agtgtgggcg agctccccag caccgtggaa tcactccaga
     5701 aggtgcagga gcaggtgcac acgctgctca gtcaggacca agcccaggcc gcccgtctgc
     5761 ctcctcagga cttcctggac agactttctt ctctagacaa cctgaaagcc tcagtcagcc
     5821 aagtggaggc ggacttgaaa atgctcagga ctgctgtgga cagtttggtt gcatactcgg
     5881 tcaaaataga aaccaacgag aacaatctgg aatcagccaa gggtttacta gatgacctga
     5941 ggaatgatct ggataggttg tttgtgaaag tggagaagat tcacgaaaag gtcggtggat
     6001 ctggaggttc tggtggaatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     6061 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6121 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6181 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6241 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6301 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6361 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6421 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6481 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6541 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6601 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6661 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6721 acaagtccgg actcagatcc gctggcccag gaacaagttt gtacaaaaaa gcaggctttc
     6781 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6841 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6901 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6961 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     7021 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     7081 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7141 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7201 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     7261 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     7321 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     7381 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     7441 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     7501 tgtacaagta gtccggactc agatccggta ccggatccgg ggttggggtt gcgccttttc
     7561 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     7621 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     7681 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     7741 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     7801 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     7861 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     7921 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     7981 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     8041 tctccccagc aattcaccat gattgaacaa gatggattgc acgcaggttc tccggccgct
     8101 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8161 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8221 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8281 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8341 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8401 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8461 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8521 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8581 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8641 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8701 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     8761 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     8821 gccttctatc gccttcttga cgagttcttc cccggtgccg aaggtagagg ttctctcctc
     8881 acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc
     8941 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     9001 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     9061 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     9121 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     9181 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     9241 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     9301 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     9361 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     9421 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     9481 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     9541 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     9601 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     9661 ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
     9721 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     9781 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     9841 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     9901 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     9961 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    10021 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
    10081 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    10141 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    10201 gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta
    10261 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    10321 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
    10381 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10441 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10501 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10561 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
    10621 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10681 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10741 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
    10801 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10861 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    10921 gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    11041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    11101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    11161 gt
//
