LOCUS       sequence-275652-       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Relb in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Relb
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2483..3071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2536..2556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2954..2965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3074..3090)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3075..3091)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3596..3776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3802..3819)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3808..4032
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4078..4506
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4165..4184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4375..4396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4515..4535)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4520..4849
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4700..4835
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4762..4781
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4897..4944
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4963..5337
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5467..5588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5504..5523)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5558..5577
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5637..5653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5637..5653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5650..5672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5661..5677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5685..5691,5692..5709,5710..5715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5730..5751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5868..5885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6039..6627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6119..6138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6798..7589,7590..7658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7421..7440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7659..7763)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7838..7857)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8029..8408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8410..8608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8626..8806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8853..8978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9471..9704
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9889..9933
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catgccgagt cgccgcgctg ccagagagtc cgcgcccgag ctaggggcct tgggttccag
      841 tgacctctct tccctgtcac taacggtctc caggaccaca gatgaattgg aaatcatcga
      901 cgaatacatt aaggagaacg gctttggcct ggacgggaca cagctgagtg agatgccgcg
      961 cctggtgccc cgcgggcccg cctcactgag cagcgtcacg ctgggccctg ctgcaccacc
     1021 gcctccggcc acgccgtcct ggagctgcac actgggcagg ctggtgtcac ccggcccgtg
     1081 cccacggccg tacctggtca tcacagagca gccaaagcag cgtggcatgc gcttccgcta
     1141 cgagtgcgag ggccgctcgg ccggcagcat cctcggggag agcagcaccg aagccagcaa
     1201 gaccctgccc gccatcgagc ttcgagactg tggcgggctg cgggaggtgg aggtgacggc
     1261 gtgcctggtg tggaaggact ggccacaccg ggtacaccca catagcctcg tggggaaaga
     1321 ctgcacggac ggcgtctgca gggtgcggct gcggcctcac gtcagccccc ggcacagctt
     1381 taacaacctg ggcatccagt gtgttaggaa gaaggaaatt gaagctgcca ttgagcggaa
     1441 gatccagctg ggaattgacc cctacaatgc tggctccctg aagaaccatc aggaggtcga
     1501 catgaatgtc gtcaggatct gcttccaggc ctcctatcgg gaccagcagg gacatctgca
     1561 ccgcatggac cccatcctct ctgagcctgt ctacgacaag aagtccacca acacatcgga
     1621 gctgcggatt tgccgaatca acaaggagag cgggccgtgc acaggtggtg aggagctgta
     1681 cttgctctgt gacaaggtgc aaaaagagga catatccgtg gtgttcagca cagcttcctg
     1741 ggaaggccgt gccgacttct ctcaagctga tgtgcaccgg cagatcgcca ttgtgttcaa
     1801 aacgccaccc tacgaggacc tggagatctc agagcccgtg actgtcaatg tgttcttgca
     1861 gcggctcacg gatggggtgt gcagcgagcc gctgcccttc acgtacctgc ctcgggatca
     1921 tgacagctac ggtgtggaca agaagcgaaa gcggggactg cctgatgtcc ttggagagtt
     1981 gagcagctct gatccacatg gaatcgagag caaacgaagg aaaaagaaac cagtgttctt
     2041 ggaccacttc ctgcctggcc acagctcagg cctgttcctc ccaccatcgg ctctgcagcc
     2101 ggcagactct gatttcttcc ctgcttccat atcccttcct gggctggagc ctcctggtgg
     2161 acccgatctc ctggacgatg gctttgccta tgatccttct gcccccacgc tcttcactat
     2221 gttggacctg ctgcccccag caccaccact tgccagtgct gtggtgggta gcgggggtgc
     2281 aggggccacc gttgtggagt cttctggccc agagccccta tcactggact cttttgcagc
     2341 gccgggcccc ggggatgttg gtactgctag ccttgtgggc agcaacatgt ttcccaacca
     2401 gtaccgagag gcagctttcg ggggtggcct cctatctcca gggcctgaag ccacgtagga
     2461 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2521 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2581 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2641 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2701 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2761 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2821 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     2881 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2941 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3001 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3061 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     3121 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     3181 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     3241 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3301 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     3361 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     3421 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     3481 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     3541 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     3601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     3781 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3841 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3901 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     3961 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4021 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4081 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4141 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4201 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4261 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4321 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4381 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4441 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4501 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4561 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4621 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4681 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4741 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4801 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4861 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     4921 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     4981 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     5041 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     5101 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     5161 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     5221 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     5281 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     5341 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5401 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5461 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5521 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5581 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     5641 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5701 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5761 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5821 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7621 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7681 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7741 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     7801 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     7861 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     7921 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     7981 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     8041 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     8101 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     8161 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     8221 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     8281 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     8341 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     8401 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     8461 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     8521 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     8581 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     8641 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8701 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8761 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     8821 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     8881 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     8941 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9001 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9061 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9121 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9181 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9241 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9301 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9361 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9421 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9481 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9541 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9601 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9661 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9721 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     9781 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9841 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     9901 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     9961 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10021 tttgctgtac tttctatagt gaatagagtt aggcagggat attc
//
