LOCUS       sequence-275678-        9923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible lentiviral expression of human REEP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann 
            JA, Corn JE
  TITLE     A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial 
            Metabolism and ER-Resident UFMylation.
  JOURNAL   Cell. 2020 Mar 19;180(6):1160-1177.e20. doi: 
            10.1016/j.cell.2020.02.017. Epub 2020 Mar 10.
  PUBMED    32160526
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.02.017"; journalName: "Cell"; date: 
            "2020-03-19- 19"; volume: "180"; issue: "6"; pages: "1160-1177.e20"
COMMENT     Sequence Label: TetOn-REEP5-mCherry-eGFP
FEATURES             Location/Qualifiers
     source          1..9923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(31..99,100..891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(249..268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1062..1650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1551..1570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1804..1821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1938..1959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1974..1979,1980..1997,1998..2004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2012..2028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2017..2039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2036..2052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2036..2052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2071..2091
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2073..2091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2119..2345
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2346..2526
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2573..2698
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3191..3424
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3609..3653
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3866..3983
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4037..4351
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4045..4063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4081..4099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4116..4134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4152..4170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4188..4206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4223..4241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4259..4277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4324..4348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4965..5672
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5110..5128)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5378..5397
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    5700..5724
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5733..5742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5739..5741,5742..5744,5745..6458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5739..5741,5742..5744,5745..6455)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5784..5805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6045..6064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6392..6413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6486..6996
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6915..6933
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7006..7797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7060..7079)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7670..7689
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             7807..7860
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7861..8478,8479..8484,8485..8520,8521..8523,8524..8559,
                     8560..8562,8563..8598,8599..8607)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8647..8667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9065..9076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9281..9514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9586..9707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9623..9642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9677..9696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9747..9882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        join(9882..9923,1..30)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
       61 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      421 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
      601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      721 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
      841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
      901 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
      961 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1201 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     1861 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     1921 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     1981 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     2041 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     2101 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     2161 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     2221 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     2281 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     2341 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2401 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2461 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2521 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     2581 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     2641 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     2701 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     2761 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     2821 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     2881 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     2941 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     3001 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     3061 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     3121 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     3181 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     3241 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     3301 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     3361 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     3421 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     3481 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     3541 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     3601 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     3661 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     3721 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     3781 attcaccatt atcgtttcag acccacctcc caaccccgag gggacaattc tcgacctcga
     3841 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3901 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3961 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     4021 cgcgaatcga tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     4081 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
     4141 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     4201 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
     4261 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
     4321 cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt ggctagcgcc accatgtctg
     4381 cggccatgag ggagaggttc gaccggttcc tgcacgagaa gaactgcatg actgaccttc
     4441 tggccaagcc cgaggccaaa accggcgtga acaggagctt catcgctctt ggtgtcatcg
     4501 gactggtggc cttgtacctg gtgttcggtt atggagcctc tctcctctgc aacctgatag
     4561 gatttggcta cccagcctac atctcaatta aagctataga gagtcccaac aaagaagatg
     4621 atacccagtg gctgacctac tgggtagagt atggtgtgtt cagcattgct gaattcttct
     4681 ctgatatctt cctgtcatgg ttccccttct actacatgct gaagtgtggc ttcctgttgt
     4741 ggtgcatggc cccgagccct tctaatgggg ctgaactgct ctacaagcgc atcatccgtc
     4801 ctttcttcct gaagcacgag tcccagatgg acagtgtggt caaggacctt aaagacaagg
     4861 ccaaagagac tgcagatgcc atcactaaag aagcgaagaa agctaccgtg aatttactgg
     4921 gtgaagaaaa gaagagcacc ggtggatctg gaggttctgg tggaatggtg agcaagggcg
     4981 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     5041 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     5101 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     5161 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     5221 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     5281 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     5341 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     5401 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     5461 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     5521 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     5581 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     5641 actccaccgg cggcatggac gagctgtaca agtccggact cagatccgct ggcccaggaa
     5701 caagtttgta caaaaaagca ggctttccgg tcgccaccat ggtgagcaag ggcgaggagc
     5761 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5821 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5881 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     5941 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     6001 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     6061 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     6121 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     6181 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     6241 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6301 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6361 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6421 ccgggatcac tctcggcatg gacgagctgt acaagtagtc cggactcaga tccggtaccg
     6481 gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     6541 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     6601 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     6661 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     6721 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     6781 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     6841 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     6901 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     6961 tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgat tgaacaagat
     7021 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     7081 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     7141 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     7201 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7261 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     7321 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7381 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7441 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7501 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     7561 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7621 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7681 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7741 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttcccc
     7801 ggtgccgaag gtagaggttc tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca
     7861 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
     7921 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     7981 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     8041 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     8101 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     8161 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     8221 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     8281 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     8341 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     8401 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     8461 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     8521 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     8581 tttgaccttg acatgctccc cgggtaacta agtaaggatc gatccaagat atcgtattct
     8641 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8701 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8761 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     8821 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     8881 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     8941 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     9001 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9061 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9121 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9181 gcctgtttcg cctcggcgtc cggactagag gtacctttaa gaccaatgac ttacaaggca
     9241 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9301 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9481 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     9541 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     9601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     9721 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9781 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9841 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctttcaaat atgtatccgc
     9901 tcatgagaca ataaccctga taa
//
