LOCUS       sequence-275685-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Tei R, Baskin JM
  TITLE     Spatiotemporal control of phosphatidic acid signaling with 
            optogenetic, engineered phospholipase Ds.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 
            10.1083/jcb.201907013.
  PUBMED    31999306
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133675. doi"; 
            pages: " 10.1083/jcb.201907013"
COMMENT     Sequence Label: Endosome-targeted optoPLD
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     614..638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             740..2233
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2267..2971
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2409..2427)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2677..2696
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4586..4642
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4649..5158
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     complement(5703..5720)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5709..5933
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6066..6085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6217..6264
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6272..7294
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6272..7294
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7424..7545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7461..7480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7515..7534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7594..7610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7594..7610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7607..7629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7618..7634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7642..7648,7649..7666,7667..7672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7687..7708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7825..7842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7996..8584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8076..8095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8755..9546,9547..9615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9378..9397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9616..9720)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9795..9814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      661 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac
      721 ttaagcttgg tacgccacca tgaagatgga caaaaagact atagtttggt ttagaagaga
      781 cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc
      841 tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg
      901 gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct
      961 cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg
     1021 tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac
     1081 cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt
     1141 gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta
     1201 ctggaggaaa tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt
     1261 gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct
     1321 ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg
     1381 gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa
     1441 gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg
     1501 ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag
     1561 agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag
     1621 agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag
     1681 tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg
     1741 caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat
     1801 gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg
     1861 gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat
     1921 ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga
     1981 caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct
     2041 tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac
     2101 cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat
     2161 cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat
     2221 gatcggagca gcaggtggag gcggttcagg cggaggtggc agcggcgtga gcaagggcga
     2281 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2341 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2401 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2461 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2521 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     2581 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     2641 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2701 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     2761 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     2821 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     2881 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     2941 ctccaccggc ggcatggacg agctgtacaa gagatctcga agcgcggccg cgggagcagg
     3001 aggagcagct cgaggcgcag cagcgggagc aggaggagca ggtcgatcgg gcggatctgc
     3061 agattcagct acaccacatt tggacgccgt tgagcaaaca ttgaggcaag tgtccccagg
     3121 cctggagggc gatgtatggg aaagaactag tggaaataaa ttggacggaa gtgccgctga
     3181 tcccagtgat tggttgttgc aaaccccagg atgttggggc gatgataaat gcgctgacag
     3241 agttgggaca aaaaggttgc tggctaagat gactgaaaat atagggaatg ccaccagaac
     3301 cgtagatatt agtacacttg cacctttccc aaacggcgca ttccaagacg ctattgtcgc
     3361 tggtttgaag gagtccgcag ctaaaggcaa taagttgaaa gttaggatac tggttggggc
     3421 tgcacccgtc taccacatga atgtaatacc ttccaagtat agggacgaat tgactgcaaa
     3481 acttggcaaa gccgcagaaa acataacttt gaacgtggct agtatgacta catcaaaaac
     3541 agctttctcc tggaaccata gcaaaatctt ggttgtagac gggcaaagtg cccttactgg
     3601 tggaataaat agctggaagg atgattactt ggatactact catccagtgt ctgatgttga
     3661 tcttgcactg accggaccag ctgcaggctc tgccggtaga taccttgaca ctttgtggac
     3721 atggacctgc caaaacaaga gtaacatcgc ttccgtctgg tttgcagctt ccggcaacgc
     3781 agggtgcatg cccactatgc ataaagatac aaaccccaag gcatcacctg ccaccgggaa
     3841 tgtcccagta atagccgttg gagggttggg cgttggcatc aaagatgtgg accccaagtc
     3901 cactttcagg cccgatctgc caacagctag cgatactaag tgtgtggtag gacttcacga
     3961 caatactaac gccgatagag actatgatac agtcaaccca gaagagagtg ccttgagagc
     4021 tcttgttgct tcagcaaaag gtcacattga aatatctcag caggatttga acgctacatg
     4081 tccacccttg cctaggtatg acattagatt gtacgatgcc ttggctgcta aaatggctgc
     4141 tggggttaag gtgagaattg tggttagcga cccagccaac aggggcgcag tgggcagtgg
     4201 gggttacagc caaataaaat cacttagtga aatatcagac actttgagaa acaggcttgc
     4261 aaatatcaca ggcggtcaac aagctgccaa aactgcaatg tgtagcaatt tgcagcttgc
     4321 aacctttaga tcctccccca acggcaaatg ggcagacggg catccttatg cccaacacca
     4381 taagttggtt tccgtcgact catccacatt ttatatcggt tcaaaaaatt tgtacccatc
     4441 ctggctgcaa gattttggct acatagtgga atccccagag gctgccaagc aactggatgc
     4501 caaattgctt gaccctcagt ggaagtactc acaggagact gcaacagtgg attacgcaag
     4561 aggaatctgc aacgctggaa gcggcgctac aaactttagt ctgctcaagc aggctggcga
     4621 tgtcgaggaa aacccaggtc ccggtaccat gaatggagct ataggaggtg accttttgct
     4681 caattttcct gacatgtcgg tcctagagcg ccaaagggct cacctcaagt acctcaatcc
     4741 cacctttgat tctcctctcg ccggcttctt tgccgattct tcaatgatta ccggcggcga
     4801 gatggacagc tatctttcga ctgccggttt gaatcttccg atgatgtacg gtgagacgac
     4861 ggtggaaggt gattcaagac tctcaatttc gccggaaacg acgcttggga ctggaaattt
     4921 caaggcagcg aagtttgata cagagactaa ggattgtaat gaggcggcga agaagatgac
     4981 gatgaacaga gatgacctag tagaagaagg agaagaagag aagtcgaaaa taacagagca
     5041 aaacaatggg agcacaaaaa gcatcaagaa gatgaaacac aaagccaaga aagaagagaa
     5101 caatttctct aatgattcat ctaaagtgac gaaggaattg gagaaaacgg attatattca
     5161 tgtgaccggt ggcgccttag aattcgaaag tgatgccatg ttcgctgctg aaagagcccc
     5221 tgactgggtg gatgctgagg aatgccatcg gtgcagagta cagtttgggg tggtgacccg
     5281 caagcatcac tgccgagcat gtgggcagat cttctgtggc aagtgctcct ccaagtactc
     5341 caccatcccc aagttcggca ttgagaagga ggtgcgcgtg tgtgagccct gctatgagca
     5401 gctgaacaag aaggcacaag gacaaggatc cgaaagtgat gccatgttcg ctgctgaaag
     5461 agcccctgac tgggtggatg ctgaggaatg ccatcggtgc agagtacagt ttggggtggt
     5521 gacccgcaag catcactgcc gagcatgtgg gcagatcttc tgtggcaagt gctcctccaa
     5581 gtactccacc atccccaagt tcggcattga gaaggaggtg cgcgtgtgtg agccctgcta
     5641 tgagcagctg aacaagaagg catgatctag agggccctat ttcgtttaaa cccgctgatc
     5701 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5761 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5821 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5881 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5941 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6001 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6061 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6121 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6181 caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta
     6241 ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt
     6301 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     6361 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     6421 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     6481 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     6541 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     6601 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     6661 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     6721 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     6781 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     6841 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     6901 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     6961 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     7021 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     7081 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     7141 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     7201 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     7261 acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac
     7321 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7381 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7441 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7501 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7561 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9661 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9721 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     9781 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     9841 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     9901 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     9961 gatgtacggg ccagatatac gcgtt
//
