LOCUS       sequence-275695-        9625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli plasmid that contains the expression optimized genes for 
            BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen 
            DT
  TITLE     Membrane directed expression in Escherichia coli of BBA57 and other 
            virulence factors from the Lyme disease agent Borrelia burgdorferi.
  JOURNAL   Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x.
  PUBMED    31772280
REFERENCE   2  (bases 1 to 9625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-26"; volume: "9(1):17606. doi"; pages: " 
            10.1038/s41598-019-53830-x"
COMMENT     Sequence Label: pUC57-BBclone1
FEATURES             Location/Qualifiers
     source          1..9625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7417..7439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7428..7444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7452..7458,7459..7476,7477..7482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7497..7518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7635..7652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7806..8394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7886..7905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8565..9356,9357..9425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9188..9207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9426..9530)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9598..9616
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catgcgtatg ctgctggcga
      421 ccatcattct gattctgacc accggtctgc tggcggcgca gagcaaaagc aagagcatga
      481 ccgaggacga tttcgacttt gataaactgc tggcgaaaga ggagagcgtg cgtcgtctgt
      541 ttggcatcgg tttcggcgtt ggttacccgc tggcgaacat caccattagc gtgccgtatg
      601 ttgacattga tctgggttac ggtggctttg tgggcctgaa gccgaacaac ttcctgccgt
      661 atgtggttat gggtgttgat ctgctgttca aagacgaaat tcacaagaac accatgatca
      721 gcggtggcat cggcattggt gcggactgga gcaaaggcag cccggagaaa agcaacgaaa
      781 agctggagga agaggaagag aacgaggcgc agcaagtggc gagcctgcag aaccgtattg
      841 gtgtggttat ccgtctgccg ctggttattg aatacagctt tctgaaaaac atcgtgattg
      901 gcttcaaggc ggttgcgacc atcggcacca ccatgctgct gggcagcccg atgagctttg
      961 agggtgcgcg tttcaacttt ctgggcaccg gtttcatcaa gatctacatc atgatcaaga
     1021 tcttcaagaa aatttacatc ctgaccctgg tgctgggcat ggcgcacctg agctttgcga
     1081 gcgataacta catggttcgt tgcagcaagg aagaggacag caccacctgc attgcgaaac
     1141 tgaaggagat caaagaaaag aaaaactatg acctgttcag catgggtatt ggtatcggcg
     1201 acccgatcgc gaacattatg atcaccattc cgtatatcaa cattgatttt ggttacggtg
     1261 gcttcattgg cctgaagagc aacaactttg aaaactatct gaacggtggc atcgacgtta
     1321 tcttcaagaa gcagatcggt caatacatga agatcggtgg cggtatcggt attggcgcgg
     1381 attggagcaa aaccagcctg atcccgccga acgaagagga agagaccgat tacgagcgta
     1441 ttggcgcggt gatccgtatt ccgtttatca tggaatataa cttcgcgaaa aacctgagca
     1501 ttggtttcaa gatctatccg gcggttggtc cgaccatcct gctgaccaaa ccgagcattc
     1561 tgtttgaggg tatcaagttc aacttctttg gtttcggctt tattaaattc gcgtttaaca
     1621 tgaagagcca catcctgtac aaactgatca tttttctgac caccagcgcg gcgatctttg
     1681 cggcggatgc gctgaaagaa aaggacatct tcaaaattaa cccgtggatg ccgaccttcg
     1741 gttttgagaa caccagcgaa tttcgtctgg acatggatga gctggtgccg ggcttcgaaa
     1801 acaaaagcaa gatcaccatt aaactgaagc cgtttgaggc gaacccggaa ctgggcaagg
     1861 acgatccgtt cagcgcgtac attaaagtgg aggatctggc gctgaaggcg gaaggtaaga
     1921 aaggcgacca attcaaaatc gatgttggcg acattaccgc gcagatcaac atgtacgact
     1981 tctttatcaa gattagcacc atgaccgact tcgattttaa caaagagagc ctgttcagct
     2041 ttgcgccgat gaccggtttt aagagcacct actatggctt cccgagcaac gatcgtgcgg
     2101 ttcgtggcac catcctggcg cgtggcacca gcaaaaacat cggcaccatt caactgggct
     2161 acaaactgcc gaagctggac ctgacctttg cgatcggtgg caccggcacc ggtaaccgta
     2221 accaggaaaa cgacaaggat accccgtaca acaaaaccta tcagggcatt ctgtatggta
     2281 tccaagcgac ctggaaaccg attaagaacc tgctggatca gaacgaggac accaagagcg
     2341 tgatcgcgga gaccccgttt gaactgaact tcggtctgag cggcgcgtat ggtaacgaaa
     2401 cctttaacaa cagcagcatc acctacagcc tgaaagataa gagcgtggtt ggtaacgacc
     2461 tgctgagccc gaccctgagc aacagcgcga ttctggcgag cttcggtgcg aaatataagc
     2521 tgggcctgac caaaattaac gataagaaca cctacctgat cctgcagatg ggcaccgatt
     2581 ttggtatcga cccgttcgcg agcgacttta gcattttcgg tcacatcagc aaggcggcga
     2641 acttcaagaa agagaccccg agcgatccga acaagaaagc ggaaatcttt gacccgaacg
     2701 gtaacgcgct gaatttcagc aaaaacaccg agctgggcat tgcgtttagc accggcgcga
     2761 gcatcggttt cgcgtggaac aaagataccg gcgagaagga aagctgggcg atcaagggca
     2821 gcgatagcta cagcacccgt ctgtttggtg aacaagacaa gaaaagcggc gtggcgctgg
     2881 gtattagcta tggccagaac ctgtaccgta gcaaagacac cgagaaacgt ctgaagacca
     2941 ttagcgaaaa cgcgttccaa agcctgaacg ttgagatcag cagctatgaa gataacaaga
     3001 aaggtatcat caacggtctg ggctggatca ccagcattgg cctgtatgac attctgcgtc
     3061 agaaaagcgt ggagaactac ccgaccacca tcagcagcac caccgagaac aaccagaccg
     3121 aacaaagcag caccagcacc aagaccacca ccccgaacct gaccttcgag gatgcgatga
     3181 aactgggtct ggcgctgtac ctggactatg cgatcccgat tgcgagcatc agcaccgaag
     3241 cgtacgtggt tccgtatatt ggtgcgtaca tcctgggccc gagcaacaag ctgagcagcg
     3301 atgcgaccaa aatctacctg aagaccggtc tgagcctgga gaaactgatt cgttttacca
     3361 ccatcagcct gggctgggac agcaacaaca tcattgaact ggcgaacaag aacaccaaca
     3421 acgcggcgat tggtagcgcg tttctgcaat tcaaaatcgc gtacagcggt agcatgaacg
     3481 gcaaactgcg taaggcgctg aaaatcgcga ttttcaccac cctgctgctg gtgatcagct
     3541 gcaacgcgaa catggatacc aacgacaaaa acaaggcgct gaacgagtat aaactgaaga
     3601 acattagcga agttatcaag aacagcctgc agctggagag cgacccgaaa ctgaagaaag
     3661 agccggaaag caacatcaac caaagcaccc cgccgatcct ggagattgaa aaaatcgagc
     3721 cgggtaaaca ggaaatgagc ctgaagagcg agttcggcag cgaaagcctg atgccgctgg
     3781 aagagccgga agaggcgaac atggcgaaaa gcgaagagga aattgcgaaa atccaggaga
     3841 agctgctgct gattggtgcg agcgatgaaa tcaccgacca ggagctgggc gaaaacatgc
     3901 aaaagtttct gaacccgacc accgttgagt tcaaaatcag caccaccacc aacgaaacca
     3961 ttctgaccac catcgaggaa gaggaaatca acaacaacaa ggataagatc ttcgaccaca
     4021 aagaggagaa cgtgaccctg ggtaacaaca gcctggagaa cgcgaccctg aacaagaaca
     4081 ccattaccct ggcgcagaac caaaaatata ccacccacct gaaaaacgac gataagttta
     4141 tcaccaaaga atacctgaag caagttcgtg atagcctgga caaggcgctg aacgcgatta
     4201 aaaacctgga gaccagcaag gagttccgtg agctggaaaa cctgccgaaa gatcaggaga
     4261 acagcccgag cgcgaagaaa gagagcaccg aaagcaacat taacaacacc aacaccgaga
     4321 acatcgaaat cattaaaaac cgtctgctgg aggaactgaa caagagcgag ctgatcttcg
     4381 aagataccaa agacccgctg ggcaccagca ccattaagaa agtgatcgac gcggcgaaga
     4441 aatggcagga gaaggaaaac agcagccaaa tcgactggga tctgggcttc aaatttcacc
     4501 cgaacccgaa attttataac aagctgaccg cgcaagagta caaagtgctg gcggaaaagt
     4561 tcaccaaagt taagaacgag tacaagaaca ccaaagagca gctgaaagcg gaaagcaagc
     4621 tgaccaccaa cagcatcagc aaaatcatta acgcgaccaa ggagttcgcg aaccaagtga
     4681 ttaacctgat cctgctggtt gagaagaact accagatgga aaagaaattc tttagcggtt
     4741 ttctgctgag cttcctggcg ctgagcattg gtttctttat cggcatgcac tatctggcga
     4801 gcaaccgtag caacatcgtg tttgcggagg aaaaagataa caccgttcgt gcgctgcaag
     4861 acagcttccg tgaggtgagc aagaaaattc tgccgagcag cgttgaagtg cacgcgaccg
     4921 gtgttattaa gcagagcttt ccgatcccgt tctttttctt tgatatgccg gagttcgaca
     4981 gcgaacgtaa aagcaactgg gcgggtagcg gcgttatcat tggccgtgac agccagaaga
     5041 aaagcctgtt ttacgtggtt accaacagcc acgtggttga taaggcgacc gagctggaag
     5101 tggttagcta tgacaagaaa aagcacaaag cgaagctgat cggtaaagat gagaaaaagg
     5161 acattgcgct gatcagcttc gaaagcgacg atgcgaccat taaggtggcg gatctgggcg
     5221 acagcgataa actggagatc ggtgactggg tgatggcggt tggcagcccg ttccaattta
     5281 gcttcaccgt gaccgcgggt atcgttagcg gtctgcagcg tagcgcgaac ccgaacctgc
     5341 agagccgtaa cctgttcatt caaaccgatg cggcgatcaa ccgtggtaac agcggcggtc
     5401 cgctggtgaa catcaagggt gaggttatcg gcattaacgc gtggattgcg agcaacagcg
     5461 gcggtaacat cggtctgggc tttgcgattc cggtgaacaa catcaaaagc accgttgact
     5521 tctttctgaa gggtaaaaag attgaaagcg cgtggctggg catcagcttc tacccgctga
     5581 aaacccgtga tagcgaggtg ctgaagagcc tgggtgtgga aagcaacgac gttagcgcgg
     5641 cgatcattgc gagcctgtat ccgggcagcc cggcggttaa gagcggtctg cgtgcgggcg
     5701 acatcattat gaaagtgaac ggtgttagca tgagcgtgtt tcaagatgtt accagctata
     5761 ttagcgactt ctacgcgggc gagaaggtta acgtggaaat cctgcgtggc aacgtgaaaa
     5821 agaacatcga gattgtgctg gcggttcgtc cgaaagataa ggagctgagc agcagcaaga
     5881 tgctgccggg ttttgtggtt tacccgctgg ttgaggacat caaagcgcag ctgaacctgc
     5941 gtaactggat taagggtgtg gttgtggatt acatcgacaa aaacctggcg agcaacatta
     6001 aaatgaagag cggcgacgtg atcctgagcg ttaacagcaa aagcgtgagc aacctgcgtg
     6061 agttctatga tgcgctggaa gttggtaaaa acacctacaa gatcctgcgt ggcaacgaca
     6121 gctttaagat taccttcatg cgtaacaaaa acatctttaa gctgttcttt gcggcgatgc
     6181 tgttcgtgat ggcgtgcaaa gcgtacgttg aggagaagaa ggagatcgat agcctgatgg
     6241 aagacgtgct ggcgctggtt aacgacagca gcggcggtaa attcaaggat tacaaggaca
     6301 agatcaacga gctgaaagaa aacctgaagg acatcggtaa cgcggagctg aaagaaaagc
     6361 tgctgaacct gcagaacagc ttccaagaca aactggcggc gaagctggcg gcgctgaagg
     6421 cggcgaaaaa caccattgag aacatcaccg acaaggatca agacattagc aagcgtaaga
     6481 tctggagcga agcgaaactg gttggtgtga ccgttccgct gctgggcagc aacaccagcg
     6541 gtaacggcga taagatgagc aaaaacgcgg tggagcagat tgacaaggtt atcaaattcc
     6601 tggaggaagg caccaacatg cgtcgtctgt tcctgctgta tatcctgtgc agctttgttt
     6661 tcctgaacct gtttgcgcaa ggtagcagca gctacattga caaacagaag gagctggcga
     6721 tcttctacta tgaagtgggc caacgttaca tcaacgttgg caagatcaaa aagggcaagc
     6781 tgtttcaggc gaaggcgctg aaaatttacc cggatctgaa aaagggtttc gacatcaaac
     6841 tggcggtgaa ggagctggat gcgcgtatta aagacgataa cccgaaggtt gtgatgctgg
     6901 aagacatcaa gctggaggaa attccgggca tcgttcacga gaagatcgaa atcaacgatt
     6961 tcaccaacgc gccgaagatt gagtacatcg cgcagcgtga acgtagcaag aaccaagaca
     7021 agatcatcaa gttccagttc ggcaagttcg cgcgtgcgct gattagccgt aactttgacc
     7081 tgttcgacag cgtgatcgcg gataaggtga acgttatggg ccaatttgaa agcaaaaacg
     7141 acttcatcag caccctgagc agcgcgagca gcaaagcgga tgcggacgag ctggaatacc
     7201 tgagcgtgga cgattactat gatctgaaga gcctgaaaat cagcaagagc aacgacacca
     7261 gcttcgcggt gaacgttaac gcgaaaaaga acgacgttac caaaaacttt ccgttctgga
     7321 aggagcgtca gaccctgatt tttaccaccg aagacgataa caactggttc ctgagcagca
     7381 tcaacaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8341 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8401 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8461 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8521 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8581 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8641 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8701 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8761 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8821 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8881 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8941 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9001 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9061 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9121 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9181 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9241 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9301 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9361 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9421 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9481 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9541 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     9601 aggcgtatca cgaggccctt tcgtc
//
