LOCUS       sequence-275696-        9984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Tei R, Baskin JM
  TITLE     Spatiotemporal control of phosphatidic acid signaling with 
            optogenetic, engineered phospholipase Ds.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 
            10.1083/jcb.201907013.
  PUBMED    31999306
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133675. doi"; 
            pages: " 10.1083/jcb.201907013"
COMMENT     Sequence Label: Endosome-targeted optodPLD
FEATURES             Location/Qualifiers
     source          1..9984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     614..638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             740..2233
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2267..2971
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2409..2427)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2677..2696
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4586..4642
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4649..5158
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     complement(5702..5719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5708..5932
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6065..6084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6216..6263
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6271..7293
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6271..7293
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7423..7544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7460..7479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7514..7533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7593..7609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7593..7609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7606..7628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7617..7633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7641..7647,7648..7665,7666..7671))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7686..7707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7824..7841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7995..8583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8075..8094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8754..9545,9546..9614))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9377..9396
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9615..9719)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9794..9813)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      661 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac
      721 ttaagcttgg tacgccacca tgaagatgga caaaaagact atagtttggt ttagaagaga
      781 cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc
      841 tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg
      901 gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct
      961 cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg
     1021 tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac
     1081 cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt
     1141 gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta
     1201 ctggaggaaa tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt
     1261 gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct
     1321 ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg
     1381 gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa
     1441 gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg
     1501 ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag
     1561 agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag
     1621 agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag
     1681 tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg
     1741 caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat
     1801 gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg
     1861 gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat
     1921 ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga
     1981 caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct
     2041 tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac
     2101 cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat
     2161 cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat
     2221 gatcggagca gcaggtggag gcggttcagg cggaggtggc agcggcgtga gcaagggcga
     2281 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2341 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2401 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2461 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2521 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     2581 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     2641 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2701 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     2761 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     2821 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     2881 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     2941 ctccaccggc ggcatggacg agctgtacaa gagatctcga agcgcggccg cgggagcagg
     3001 aggagcagct cgaggcgcag cagcgggagc aggaggagca ggtcgatcgg gcggatctgc
     3061 agattcagct acaccacatt tggacgccgt tgagcaaaca ttgaggcaag tgtccccagg
     3121 cctggagggc gatgtatggg aaagaactag tggaaataaa ttggacggaa gtgccgctga
     3181 tcccagtgat tggttgttgc aaaccccagg atgttggggc gatgataaat gcgctgacag
     3241 agttgggaca aaaaggttgc tggctaagat gactgaaaat atagggaatg ccaccagaac
     3301 cgtagatatt agtacacttg cacctttccc aaacggcgca ttccaagacg ctattgtcgc
     3361 tggtttgaag gagtccgcag ctaaaggcaa taagttgaaa gttaggatac tggttggggc
     3421 tgcacccgtc taccacatga atgtaatacc ttccaagtat agggacgaat tgactgcaaa
     3481 acttggcaaa gccgcagaaa acataacttt gaacgtggct agtatgacta catcaaaaac
     3541 agctttctcc tggaacgcta gcaaaatctt ggttgtagac gggcaaagtg cccttactgg
     3601 tggaataaat agctggaagg atgattactt ggatactact catccagtgt ctgatgttga
     3661 tcttgcactg accggaccag ctgcaggctc tgccggtaga taccttgaca ctttgtggac
     3721 atggacctgc caaaacaaga gtaacatcgc ttccgtctgg tttgcagctt ccggcaacgc
     3781 agggtgcatg cccactatgc ataaagatac aaaccccaag gcatcacctg ccaccgggaa
     3841 tgtcccagta atagccgttg gagggttggg cgttggcatc aaagatgtgg accccaagtc
     3901 cactttcagg cccgatctgc caacagctag cgatactaag tgtgtggtag gacttcacga
     3961 caatactaac gccgatagag actatgatac agtcaaccca gaagagagtg ccttgagagc
     4021 tcttgttgct tcagcaaaag gtcacattga aatatctcag caggatttga acgctacatg
     4081 tccacccttg cctaggtatg acattagatt gtacgatgcc ttggctgcta aaatggctgc
     4141 tggggttaag gtgagaattg tggttagcga cccagccaac aggggcgcag tgggcagtgg
     4201 gggttacagc caaataaaat cacttagtga aatatcagac actttgagaa acaggcttgc
     4261 aaatatcaca ggcggtcaac aagctgccaa aactgcaatg tgtagcaatt tgcagcttgc
     4321 aacctttaga tcctccccca acggcaaatg ggcagacggg catccttatg cccaacacca
     4381 taagttggtt tccgtcgact catccacatt ttatatcggt tcaaaaaatt tgtacccatc
     4441 ctggctgcaa gattttggct acatagtgga atccccagag gctgccaagc aactggatgc
     4501 caaattgctt gaccctcagt ggaagtactc acaggagact gcaacagtgg attacgcaag
     4561 aggaatctgc aacgctggaa gcggcgctac aaactttagt ctgctcaagc aggctggcga
     4621 tgtcgaggaa aacccaggtc ccggtaccat gaatggagct ataggaggtg accttttgct
     4681 caattttcct gacatgtcgg tcctagagcg ccaaagggct cacctcaagt acctcaatcc
     4741 cacctttgat tctcctctcg ccggcttctt tgccgattct tcaatgatta ccggcggcga
     4801 gatggacagc tatctttcga ctgccggttt gaatcttccg atgatgtacg gtgagacgac
     4861 ggtggaaggt gattcaagac tctcaatttc gccggaaacg acgcttggga ctggaaattt
     4921 caaggcagcg aagtttgata cagagactaa ggattgtaat gaggcggcga agaagatgac
     4981 gatgaacaga gatgacctag tagaagaagg agaagaagag aagtcgaaaa taacagagca
     5041 aaacaatggg agcacaaaaa gcatcaagaa gatgaaacac aaagccaaga aagaagagaa
     5101 caatttctct aatgattcat ctaaagtgac gaaggaattg gagaaaacgg attatattca
     5161 tgtgaccggt ggcgccttag aattcgaaag tgatgccatg ttcgctgctg aaagagcccc
     5221 tgactgggtg gatgctgagg aatgccatcg gtgcagagta cagtttgggg tggtgacccg
     5281 caagcatcac tgccgagcat gtgggcagat cttctgtggc aagtgctcct ccaagtactc
     5341 caccatcccc aagttcggca ttgagaagga ggtgcgcgtg tgtgagccct gctatgagca
     5401 gctgaacaag aaggcacaag gacaaggatc cgaaagtgat gccatgttcg ctgctgaaag
     5461 agcccctgac tgggtggatg ctgaggaatg ccatcggtgc agagtacagt ttggggtggt
     5521 gacccgcaag catcactgcc gagcatgtgg gcagatcttc tgtggcaagt gctcctccaa
     5581 gtactccacc atccccaagt tcggcattga gaaggaggtg cgcgtgtgtg agccctgcta
     5641 tgagcagctg aacaagaagg catgatctag agggccctat tcgtttaaac ccgctgatca
     5701 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5761 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5881 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5941 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6181 aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat
     6241 tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc
     6301 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     6361 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     6421 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     6481 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     6541 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     6601 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     6661 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     6721 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     6781 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     6841 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     6901 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     6961 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     7021 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     7081 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     7141 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     7201 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     7261 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc
     7321 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7381 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7441 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7501 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7561 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7621 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7681 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7741 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7801 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7921 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8401 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8641 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8701 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8761 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8821 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8881 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8941 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9001 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9061 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9121 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9181 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9241 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9301 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9361 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9421 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9481 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9541 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9601 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9661 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9721 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     9781 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9841 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     9901 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     9961 atgtacgggc cagatatacg cgtt
//
