LOCUS       sequence-275707-       11716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of rat Myo 9b (Myr 5) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Reinhard J, Scheel AA, Diekmann D, Hall A, Ruppert C, Bahler M
  TITLE     A novel type of myosin implicated in signalling by rho family 
            GTPases.
  JOURNAL   EMBO J. 1995 Feb 15;14(4):697-704.
  PUBMED    7882973
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1995-02-15"; volume: "14(4)"; pages: "697-704"
COMMENT     Sequence Label: GFP-Myo 9b
FEATURES             Location/Qualifiers
     source          1..11716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8504..8625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8541..8560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8595..8614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8632..9087)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8769..8790)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8981..9000
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9114..9218
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9220..9577
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9220..9411
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9243..9263)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9428..9563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9612..10406
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9612..10406
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9666..9685)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10276..10295
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10594..10613)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10638..10685
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11014..11602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11503..11522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggg agtgctcacg aggctggcag ctcaggccga
     1381 aggcggccgg ccaccttcca cctgcacatc taccctcagc tgcccagtgc tgggagccag
     1441 acctcatgcc gtgtaaccgc caccaaggac agcacaacaa gcgatgtcat ccgagacgtg
     1501 gtggccagtc tacacctgga cggctcgaag cactatgtgc tggtggaggt gaaggagtcg
     1561 ggtggggagg agtgggtgct ggatgccagc gactcgcctg tgcaccgagt gctgctgtgg
     1621 cctcggcgag cgcagaagga gcaccctcgg gaggacggct actacttcct gctgcaggag
     1681 cgcaacgctg acggcagcat tcagtacctg cacgtgcagc tgctggctca gcccacagcc
     1741 gcatgtcgcc tggtggagcg agggctgctg ccaaggcctc aagcggactt cgacgacctg
     1801 tgcaacctgc cggagctgaa cgaggccaac ctgctgcaga gcctgaagct gcgcttcgtg
     1861 cagcagaaga tctacacgta cgcgggcagc atcctggtgg ccatcaaccc cttcaagttc
     1921 ctgcccattt acaaccccaa gtatgtgaag atgtacgaga accagcagct gggaaagctg
     1981 gagccgcacg tgttcgctct ggccgatgta gcctactaca ccatgctgcg caagcacgtg
     2041 aaccagtgca tcgtcatctc tggtgagagc gggtccggca agacgcaaag caccaacttc
     2101 ctcatccact gcctcacagc gctcagccag aagggctacg ccagcggcgt cgagaggacc
     2161 atcctgggtg cagggcctgt gctggaggct tttgggaacg ccaagacagc ccacaacaac
     2221 aactccagcc gcttcgggaa gttcatccaa gtcaactacc tggagagtgg catcgtgagg
     2281 ggagctgttg tggaaaaata ccttcttgaa aagtctcgcc tggtttccca ggagaaggat
     2341 gagcggaact accatgtgtt ttattatctg ctgctgggcg tcagtgagga agagcgtcag
     2401 gaatttcagc tgaagcagcc tcaagactat ttctacctca accagcataa cttgaatatt
     2461 gaagatggag aagacctcaa acatgacttt gaaaggcttc agcaggccat ggagatggtg
     2521 ggcttcctac ctgccacgaa gaagcagatc ttctctgtcc tctcagccat cctgtatctt
     2581 ggcaatgtca cctataagaa gagagccaca ggccgggatg aaggcctgga ggtcgggccc
     2641 ccggaggtat tggacacact atcccagctc ctgaaggtaa agcgggagac cctggtggag
     2701 gtcctaacca agagaaaaac aatcacggtc aatgacaaac tcatcctgcc ttacagcctc
     2761 agtgaggcta tcactgcacg agactccatg gctaagtctc tgtacagcgc cctgtttgac
     2821 tggattgtgc tgaggatcaa ccacgccctc ctcaacaaga aggacatgga agaggctgtt
     2881 tcttgcttgt ccattggcgt cctggacata ttcggatttg aggactttga acggaacagc
     2941 ttcgagcagt tttgcatcaa ctacgccaac gagcagttgc agtactactt cacccagcac
     3001 atcttcaagc tggagcagga ggagtaccag ggtgagggta tctcgtggca caacattgac
     3061 tacaccgaca acgtgggctg tatccacctc atcagcaaga agcccactgg cctcttctac
     3121 ctgctggacg aggaaagcaa ctttccccat gccacaagcc acactctgct ggccaaattc
     3181 aagcagcagc atgaggacaa caagtacttc ctgggcacac cagtcctgga gcccgccttc
     3241 atcatccagc acttcgcagg cagagtaaag taccagatca aggacttccg ggagaagaac
     3301 atggactaca tgcggcctga catcgtggca ctgctaaggg gcagtgacag ctcctatgtg
     3361 cgccaactca tcggcatgga cccggtagct gtgttccgct gggctgtatt acgggcagcc
     3421 atcagggcca tggctgtgct gcgggaggct gggcgcctgc gtgcagagag agcagagaaa
     3481 gcagaagcag gtgtaagtag tcctgtcatt cgaagtcacg tggaagagct gccaagagga
     3541 gccaacaccc cttcagagaa actgtaccgc gatttgcata accaaatcat caagagcctc
     3601 aaaggactgc catggcaggg cgaggacccg cggaggcttc tccagtccct cagtcggctc
     3661 cagaagcccc gcaccttctt cctgaagagt aaaggtatca aacaaaagca gatcattccc
     3721 aagaacctgc tggactctaa gtccctgagg ctcatcatca gcatgacgct gcatgaccga
     3781 actaccaagt cactgctgca cctgcacaag aagaagaagc cgcccagcat cagtgcacag
     3841 ttccagacat ctcttaacaa gctgttggag gcgctgggca aggctgagcc cttcttcatc
     3901 cgctgcattc gctccaatgc cgagaagaag gagctctgct ttgatgatga gctggtgctt
     3961 cagcaactgc gctacacagg catgctggag accgtgcgca tccggcgctc tggctacagc
     4021 gccaagtaca ccttccagga cttcacggag cagttccagg tgctgctgcc caaggatgtc
     4081 cagccctgta gggaggccat tgctgccctg ttggagaagc tgcaggtgga caggcagaac
     4141 taccagatcg ggaagacgaa ggtcttcctg aaggagacag agcggcaggc cctgcaggag
     4201 aggctgcatg gtgaggtctt acgcaggatc ctgctgctgc agagttggtt ccggatggtg
     4261 ctggaacgca ggcactttgt gcagatgaag catgctgccc tgaccatcca ggcctgctgg
     4321 cggtcttatc gtgtgcgccg tacactggag aggacgcggg cagctgtgta tcttcaggct
     4381 gcctggagag gctacctgca gagacaggcc taccaccacc agaggcatag catcatccgc
     4441 ctgcagagcc tctgccgtgg ccacctacag cgcaggagct tcagccagat gatgttagag
     4501 aagcagaagg cagagcaagc cagggagact gcaggagcag agatgtcaga gggagagccc
     4561 agccctgtgg ccgctgggga gcagccgtct gagcaccctg tggaggaccc tgagagcctg
     4621 ggtgtggaga ctgagacctg gatgaacagc aagtccccaa atggcttgtc acctaagaag
     4681 gagatcccca gcccggagat ggagacccca gcccaaaaaa cagtgccagc tgaaagtcat
     4741 gagaaagtcc ccagtagccg ggagaagcga gagtcacggc ggcagcgagg gttggagcat
     4801 gttgaacgac agaacaaaca catccaatcc tgcagggagg agaatagcac cctccgagaa
     4861 ccttccagaa aggcaagcct ggaaacaggg gagagcttcc ctgaggacac aaaggagccc
     4921 agagaagatg gacttgagac atggactgag accgcagccc cctcttgtcc aaagcaggtg
     4981 ccgattgtgg gagatccgcc taggagtccc agtcccttgc agaggcccgc cagcctggac
     5041 ctagacagca gggttagccc agtgctcccc agcagctccc tggaatcccc ccaggatgag
     5101 gacaagggtg agaacagcac caaggttcag gacaagcccg agagtcccag tggctccacc
     5161 cagatccaac ggtaccaaca cccggacaca gagcggctag ccactgctgt ggagatatgg
     5221 cgaggcaaga agctcgccag tgccatgctg agccaatccc tggacctgag tgagaagccc
     5281 cggactgcag gggcagccct gactcccaca gaggagaggc gcatctcctt ctccaccagt
     5341 gatgtctcca agctgtcccc ggtcaagact tcaactgaag tcgatgggga tctgagcgcc
     5401 aagaagccag ccggccataa gaagaagtca gaagacccat ctgctggtcc cgatgcaggc
     5461 ctgcccacag gctcccaggg tgactctaaa tctgccttta agcgactctt cctgcacaaa
     5521 gccaaggata agaagcccag cctggagggt gtggaggaga cagagggcag tggagggcag
     5581 gctgcacagg aggccccggc caggaagact ctagatgtac cttctagcca gcagcaccgc
     5641 cacaccacag gcgagaagcc tctaaaaggg aagaagaacc gaaatcgtaa ggtcggacag
     5701 atcacagtgt ccgagaagtg gcgagagtcg gtgttccgta agatcactaa tgccaacgag
     5761 ctcaagtttc tggatgagtt cctgctcaac aaggtgaatg accttcgctc acagaagaca
     5821 cccatcgaga gcttgttcat tgaggccact gagcgtttca ggagcaatat caagaccatg
     5881 tattctgtgc ctaacgggaa gatccatgta ggctacaagg acttgatgga gaactaccag
     5941 atcgttgtca gtaacctggc tgccgagcgt ggggagaagg acaccaacct ggtcctcaat
     6001 gtcttccagt cactgctgga tgaattcact cgcagctaca acaagactga ctttgagccg
     6061 gtcaagggca aagcccagaa gaagaagagg aagcaggagc gtgctgtcca ggaacacaat
     6121 ggacatgtgt ttgccagcta ccaagtgaac atcccgcagt cgtgtgagca gtgtctgtcc
     6181 tacatctggc tcatggacaa ggccctgcta tgcagtgtgt gcaagatgac ctgccacaag
     6241 aaatgtgtgc acaagattca gagctattgc tcctacactg gacggaggaa gagtgagctg
     6301 ggtgccgaac caggccactt cggcgtgtgt gtagacagcc tgaccagtga caaggcctcc
     6361 gtgcccattg tgctggagaa gcttctggaa catgtagaga tgcatggcct gtataccgag
     6421 ggtctttacc gcaagtcagg cgctgccaac cggacacggg aattacgcca ggctctgcag
     6481 acagaccctg ctacagttaa gctggaggac ttccccatcc acgccatcac cggggtcctg
     6541 aagcaatggc tgcgggagct gcctgagcca ctcatgactt ttgcccagta tggagacttc
     6601 ctcagggctg ttgagcttcc agagaagcag gagcagctgg ctgccatcta tgcagtcctg
     6661 gaccacctgc ctgaagccaa ccacacctcc ctggagaggc tcatcttcca ccttgtcaaa
     6721 gtggccctgc ttgaagatgt gaaccgcatg tctccgggag ctctagctat catcttcgcg
     6781 ccctgcctgc ttcgctgccc tgacaactcc gaccccctga ccagcatgaa ggacgtacta
     6841 aagattacca cgtgtgtgga gatgctcatc aaggaacaga tgaggaaata caaggtgaag
     6901 atggaggaga tcaaccacct ggaggcagct gagagcattg cgttccgcag gctctccctg
     6961 ctgaggcaga atgccccgtg gcctctcaaa ctggggtttt cgtctcccta tgagggggtc
     7021 cggaccaaaa gccccaggac cccagtggtc caagacttgg aggagctggg ggctctcccc
     7081 gaggaggcgg caggtggtga cgaggaccgg gaaaaggaga ttctcatgga gaggattcag
     7141 tccatcaaag aagagaagga ggacatcaca tatcgactgc cggagctgga cccacggggt
     7201 tctgacgagg agaaccttga ctcagagaca tcagccagca ctgagagcct gctggaggag
     7261 agggccgtgc ggggggccgc agaagagtga agacaccccg gaggaccccc atcatgccca
     7321 tggccaacat caagctccct ccgggcctgc ccttgcacct gacgagctgg gcacctgctc
     7381 tccaggaggc tgctgttcca gtgaagcgcc gagagccacc tgcccgccga caggaccagg
     7441 tacattccgt atacatcgcc cctggagctg acctgccatc gcagggtaca ctaggacccc
     7501 tggaccacca tgatgccata cttcctgggg ccaaacgcag gtattcagac ccccctacct
     7561 actgcctgcc ctccagctcc agccaagcca atggctgagg accatgactg gccatctgcg
     7621 tgttctaaaa tccccgaact ggcatcccag ctgtggagcc ggccttcact ctctgagaag
     7681 gatctagaat gaaaagttcc cagagggatg ctgtggccag ctctgtgtgt tgtggaggct
     7741 ggggactgct ggccaggacc atcagagccc caacctgcac agcagtcccc cccccccctt
     7801 tgtacttttc agtaactgtt tctctttttg tggtttacat aacttttaag ttcataacag
     7861 ccttaatgga ggaccaaact ttttgtacgt gtgtctgagc tttgatatta actgtccacc
     7921 gctgtaacct ggacatgggc aggactagcc tgcaaagtgg atatgtgggc cacacctggc
     7981 gactgcactc cacagccatg gagagcaagc aagcaagcga acgagcggac tcacaagaca
     8041 cctcttaccc ttgaatacct cccagcagcg gtcatctggc cagagaacct tccagagatt
     8101 gtgggagctg tccacagggt cacagctgaa ggcaggacag aatgggaagt ccctgcatcg
     8161 cagggatgct ctacccactg cctgtgggct gcaaggagcc agggatcgga gctgtaggga
     8221 caagcagtgc atgtcccgaa tttcctctgc agcccccgtg tcagcctggg ttcttaagag
     8281 gctggctctc ttctcaggcc ctacccagtc ctggacacaa ggaagcccca ccggaggtgt
     8341 gaaataaaag tgcttgacaa gtgtggaatt cgatggatcc accggatcta gataactgat
     8401 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8461 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     8521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     8641 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8701 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8761 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8821 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8881 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8941 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     9001 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     9061 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     9121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9181 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     9241 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9301 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9361 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9421 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9481 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9541 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     9601 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     9661 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     9721 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     9781 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     9841 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     9901 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     9961 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    10021 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    10081 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    10141 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    10201 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    10261 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    10321 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    10381 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
    10441 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    10501 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    10561 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
    10621 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
    10681 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    10741 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    10801 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
    10861 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    10921 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    11041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    11101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    11161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    11221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    11281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    11341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    11401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    11461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    11521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    11581 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    11641 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    11701 tattaccgcc atgcat
//
