LOCUS       sequence-275727-       10308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter expression plasmid for 
            TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Grunewald J, Zhou R, Lareau CA, Garcia SP, Iyer S, Miller BR, 
            Langner LM, Hsu JY, Aryee MJ, Joung JK
  TITLE     A dual-deaminase CRISPR base editor enables concurrent adenine and 
            cytosine editing.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 
            10.1038/s41587-020-0535-y.
  PUBMED    32483364
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 
            10.1038/s41587-020-0535-y."
COMMENT     Sequence Label: SPACE-VRQR (pRZ5137)
FEATURES             Location/Qualifiers
     source          1..10308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     512..531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(750..854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(933..952)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1124..1503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1504..1707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1658..1678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1749..1768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1749..1767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1817..1837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2432..6532
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6575..6595
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7343..7591
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7622..7870
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7880..7936
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7937..7939,7940..7942,7943..8653)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7937..7939,7940..7942,7943..8653)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8243..8262)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8590..8611
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8666..8686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8695..8712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8735..8752)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8741..8965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9036..9052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9036..9052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9049..9071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9060..9076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9084..9090,9091..9108,9109..9114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9129..9150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9267..9284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9438..10026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9518..9537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10197..10308,1..680,681..749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
       61 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
      541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
      601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
      661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
      721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
      781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
      841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
      901 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
      961 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     1021 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     1081 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     1141 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1201 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1261 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1321 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1381 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1441 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1501 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1561 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1621 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1681 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     1741 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     1801 aagcgagttc gagtcaccaa agaagaagcg gaaagtctct gaggtggagt tttcccacga
     1861 gtactggatg agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc
     1921 tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat
     1981 cggcctgcac gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt
     2041 catgcagaac tacagactga ttgacgccac cctgtacggc acattcgagc cttgcgtgat
     2101 gtgcgccggc gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc
     2161 aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg
     2221 cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt
     2281 tcggatgcct agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgactccgg
     2341 aggatctagc ggaggctcct ctggctctga gacacctggc acaagcgaga gcgcaacacc
     2401 tgaaagcagc gggggcagca gcggggggtc agacaagaag tacagcatcg gcctggccat
     2461 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
     2521 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
     2581 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
     2641 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     2701 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     2761 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     2821 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     2881 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     2941 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     3001 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     3061 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     3121 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     3181 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     3241 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     3301 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     3361 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     3421 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     3481 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     3541 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     3601 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     3661 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     3721 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     3781 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     3841 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     3901 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     3961 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     4021 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     4081 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     4141 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     4201 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     4261 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     4321 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     4381 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     4441 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     4501 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     4561 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     4621 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     4681 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     4741 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     4801 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     4861 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     4921 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     4981 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     5041 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     5101 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     5161 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     5221 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     5281 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     5341 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     5401 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     5461 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     5521 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     5581 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     5641 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     5701 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     5761 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     5821 cggcggcttc gtgagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     5881 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     5941 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     6001 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     6061 gagaatgctg gcctctgcca gagaactgca gaagggaaac gaactggccc tgccctccaa
     6121 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     6181 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     6241 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     6301 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     6361 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     6421 cgaccggaag cagtacagaa gcaccaaaga ggtgctggac gccaccctga tccaccagag
     6481 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg actctggcgg
     6541 ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctctgg
     6601 aggatctagc ggaggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc
     6661 agagagctcc ggcggctcct ccggaggatc cacagacgcc gagtacgtgc gcattcatga
     6721 gaaactggat atttacacct tcaagaagca gttcttcaac aacaagaaat ctgtgtcaca
     6781 ccgctgctac gtgctgtttg agttgaagcg aaggggcgaa agaagggctt gcttttgggg
     6841 ctatgccgtc aacaagcccc aaagtggcac cgagagagga atacacgctg agatattcag
     6901 tatccgaaag gtggaagagt atcttcggga taatcctggg cagtttacga tcaactggta
     6961 ttccagctgg agtccttgcg ctgattgtgc cgagaaaatt ctggaatggt ataatcagga
     7021 acttcgggga aacgggcaca cattgaaaat ctgggcctgc aagctgtact acgagaagaa
     7081 tgcccggaac cagataggac tctggaatct gagggacaat ggtgtaggcc tgaacgtgat
     7141 ggtttccgag cactatcagt gttgtcggaa gattttcatc caaagctctc ataaccagct
     7201 caatgaaaac cgctggttgg agaaaacact gaaacgtgcg gagaagtgga gatccgagct
     7261 gagcatcatg atccaggtca agattctgca taccactaag tctccagccg ttagcggcgg
     7321 gagcggcggg agcgggggga gcactaatct gagcgacatc attgagaagg agactgggaa
     7381 acagctggtc attcaggagt ccatcctgat gctgcctgag gaggtggagg aagtgatcgg
     7441 caacaagcca gagtctgaca tcctggtgca caccgcctac gacgagtcca cagatgagaa
     7501 tgtgatgctg ctgacctctg acgcccccga gtataagcct tgggccctgg tcatccagga
     7561 ttctaacggc gagaataaga tcaagatgct gagcggagga tccggaggat ctggaggcag
     7621 caccaacctg tctgacatca tcgagaagga gacaggcaag cagctggtca tccaggagag
     7681 catcctgatg ctgcccgaag aagtcgaaga agtgatcgga aacaagcctg agagcgatat
     7741 cctggtccat accgcctacg acgagagtac cgacgaaaat gtgatgctgc tgacatccga
     7801 cgccccagag tataagccct gggctctggt catccaggat tccaacggag agaacaaaat
     7861 caaaatgctg ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga
     7921 ggagaaccct ggacctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     8041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     8101 cgtgccctgg cccaccctcg tgaccaccct gacctatgga gtgcagtgct tcagccgcta
     8161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     8221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     8281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     8341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     8401 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     8461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     8521 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     8581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     8641 cgagctgtac aagtctggtg gttctcccaa gaagaagagg aaagtctaac cggtcatcat
     8701 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     8761 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8821 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8881 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8941 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata
     9001 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9061 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag
     9121 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9241 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9301 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9361 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9421 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9481 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9601 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9721 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9781 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9841 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9901 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9961 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10021 tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca
    10081 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10141 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10201 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10261 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttacca
//
