LOCUS       sequence-27573-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, 
            Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, 
            Ramalho-Santos M
  TITLE     Chd1 regulates open chromatin and pluripotency of embryonic stem 
            cells.
  JOURNAL   Nature. 2009 Aug 13;460(7257):863-8. doi: 10.1038/nature08212. Epub 
            2009 Jul 8.
  PUBMED    19587682
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08212"; journalName: "Nature"; date: "2009-08-13- 
            13"; volume: "460"; issue: "7257"; pages: "863-8"
COMMENT     Sequence Label: pSicoR-mCh-Chd1i1
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(171..374)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(200..220)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        375..678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggcccn gccctcgccc tcgaatctcg aactcgtggc cgttcacgga gccctccatg
       61 tgcacctttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      121 caccatggtg gcgaccggta gcgctagcgg atctgacggt tcactaaacc agctctgctt
      181 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      241 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      301 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      361 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      421 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      481 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      541 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      601 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      661 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      721 gatnactatt aatactaatg catggcggta atacggttat cacgcggccg cctatggatc
      781 tagtatcgag aaaaacttat gatganctaa tctctgatta ctcntcanat gaaaaagntt
      841 tnccaggaat ttnagccaaa ncnatnntta ng
//
