LOCUS       sequence-275735-        9442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a gain of function TYK2-V678F protein (correspoding to
            JAK2-V617F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Gakovic M, Ragimbeau J, Francois V, Constantinescu SN, Pellegrini S
  TITLE     The Stat3-activating Tyk2 V678F mutant does not up-regulate 
            signaling through the type I interferon receptor but confers ligand 
            hypersensitivity to a homodimeric receptor.
  JOURNAL   J Biol Chem. 2008 Jul 4;283(27):18522-9. doi: 
            10.1074/jbc.M801427200. Epub 2008 May 2.
  PUBMED    18456658
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M801427200"; journalName: "J Biol Chem"; date: 
            "2008-07-4- 4"; volume: "283"; issue: "27"; pages: "18522-9"
COMMENT     Sequence Label: pRC/CMV-TYK2V678F-VSV
FEATURES             Location/Qualifiers
     source          1..9442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..23
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3975..4007
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(4042..4060)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4043..4060)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4080..4097)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4086..4310
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4356..4869
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4443..4462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4653..4674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4892..4912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4897..5226
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5077..5212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5293..6087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5347..5366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5957..5976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6261..6395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6298..6317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6352..6371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6426..6442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6426..6442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6439..6461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6450..6466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6474..6480,6481..6498,6499..6504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6519..6540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6657..6674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6828..7416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6908..6927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7587..8378,8379..8447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8210..8229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8448..8552)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8627..8646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8818..9197
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9198..9401
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9352..9372
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattaatacg actcactata gggagaccca agcttgtcga ggaactgaaa aaccagaaag
       61 ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggatccc ccccccmccc
      121 ccaagaagcc gcggggactg gctgcgcttg acaggctgca cttggatggg agcacctggt
      181 gcctcgggac tgctccgatg cccgggtctg tgctgaatgt gtaatatgcg gaactatatt
      241 gaaacattac aaccatcttt tgatggcaac accctgagga cctccctttt ccagatgggg
      301 aaactgaggc ccagaattgc taagtggctt gcttgagttg acacagggag ctccaggact
      361 caccctcagc tgagccacct gccgggagca tgcctctgcg ccactggggg atggccaggg
      421 gcagtaagcc cgttggggat ggagcccagc ccatggctgc catgggaggc ctgaaggtgc
      481 ttctgcactg ggctggtcca ggcggcgggg agccctgggt cactttcagt gagtcatcgc
      541 tgacagctga ggaagtctgc atccacattg cacataaagt tggtatcact cctccttgct
      601 tcaatctctt tgccctcttc gatgctcagg cccaagtctg gttgccccca aaccacatcc
      661 tagagatccc cagagatgca agcctgatgc tatatttccg cataaggttt tatttccgga
      721 actggcatgg catgaatcct cgggaaccgg ctgtgtaccg ttgtgggccc ccaggaaccg
      781 aggcatcctc agatcagaca gcacagggga tgcaactcct ggacccagcc tcatttgagt
      841 acctctttga gcagggcaag catgagtttg tgaatgacgt ggcatcactg tgggagctgt
      901 cgaccgagga ggagatccac cactttaaga atgagagcct gggcatggcc tttctgcacc
      961 tctgtcacct cgctctccgc catggcatcc ccctggagga ggtggccaag aagaccagct
     1021 tcaaggactg catcccgcgc tccttccgcc ggcatatccg gcagcacagc gccctgaccc
     1081 ggctgcgcct tcggaacgtc ttccgcaggt tcctgcggga cttccagccg ggccgactct
     1141 cccagcagat ggtcatggtc aaatacctag ccacactcga gcggctggca ccccgcttcg
     1201 gcacagagcg tgtgcccgtg tgccacctga ggctgctggc ccaggccgag ggggagccct
     1261 gctacatccg ggacagtggg gtggccccta cagaccctgg ccctgagtct gctgctgggc
     1321 ccccaaccca cgaggtgctg gtgacaggca ctggtggcat ccagtggtgg ccagtagagg
     1381 aggaggtgaa caaggaggag ggttctagtg gcagcagtgg caggaacccc caagccagcc
     1441 tgtttgggaa gaaggccaag gctcacaagg cattcggcca gccggcagac aggccgcggg
     1501 agccactgtg ggcctacttc tgtgacttcc gggacatcac ccacgtggtg ctgaaagagc
     1561 actgtgtcag catccaccgg caggacaaca agtgcctgga gctgagcttg ccttcccggg
     1621 ctgcggcgct gtccttcgtg tcgctggtgg acggctattt ccgcctgacg gccgactcca
     1681 gccactacct gtgccacgag gtggctcccc cacggctggt gatgagcatc cgggatggga
     1741 tccacggacc cctgctggag ccatttgtgc aggccaagct gcggcccgag gacggcctgt
     1801 acctcattca ctggagcacc agccacccct accgcctgat cctcacagtg gcccagcgta
     1861 gccaggcacc agacggcatg cagagcttgc ggctccgaaa gttccccatt gagcagcagg
     1921 acggggcctt cgtgctggag ggctggggcc ggtccttccc cagcgttcgg gaacttgggg
     1981 ctgccttgca gggctgcttg ctgagggccg gggatgactg cttctctctg cgtcgctgtt
     2041 gcctgcccca accaggagaa acctccaatc tcatcatcat gcggggggct cgggccagcc
     2101 ccaggacact caacctcagc cagctcagct tccaccgggt tgaccagaag gagatcaccc
     2161 agctgtccca cttgggccag ggcacaagga ccaacgtgta tgagggccgc ctgcgagtgg
     2221 agggcagcgg ggaccctgag gagggcaaga tggatgacga ggaccccctc gtgcctggca
     2281 gggaccgtgg gcaggagcta cgagtggtgc tcaaagtgct ggaccctagt caccatgaca
     2341 tcgccctggc cttctacgag acagccagcc tcatgagcca ggtctcccac acgcacctgg
     2401 ccttcgtgca tggcgtctgt ttccgcggcc ctgaaaatat catggtgaca gagtacgtgg
     2461 agcacggacc cctggatgtg tggctgcgga gggagcgggg ccatgtgccc atggcttgga
     2521 agatggtggt ggcccagcag ctggccagcg ccctcagcta cctggagaac aagaacctgg
     2581 ttcatggtaa tgtgtgtggc cggaacatcc tgctggcccg gctggggttg gcagagggca
     2641 ccagcccctt catcaagctg agtgatcctg gcgtgggcct gggcgccctc tccagggagg
     2701 agcgggtgga gaggatcccc tggctggccc ccgaatgcct accaggtggg gccaacagcc
     2761 taagcaccgc catggacaag tgggggtttg gcgccaccct cctggagatc tgctttgacg
     2821 gagaggcccc tctgcagagc cgcagtccct ccgagaagga gcatttctac cagaggcagc
     2881 accggctgcc cgagccctcc tgcccacagc tggccacact caccagccag tgtctgacct
     2941 atgagccaac ccagaggcca tcattccgca ccatcctgcg tgacctcacc cggctgcagc
     3001 cccacaatct tgctgacgtc ttgactgtga acccggactc accggcgtcg gaccctacgg
     3061 ttttccacaa gcgctatttg aaaaagatcc gagatctggg cgagggtcac ttcggcaagg
     3121 tcagcttgta ctgctacgat ccgaccaacg acggcactgg cgagatggtg gcggtgaaag
     3181 ccctcaaggc agactgcggc ccccagcacc gctcgggctg gaagcaggag attgacattc
     3241 tgcgcacgct ctaccacgag cacatcatca agtacaaggg ctgctgcgag gaccaaggcg
     3301 agaagtcgct gcagctggtc atggagtacg tgcccctggg cagcctccga gactacctgc
     3361 cccggcacag catcgggctg gcccagctgc tgctcttcgc ccagcagatc tgcgagggca
     3421 tggcctatct gcacgcgcag cactacatcc accgagacct agccgcgcgc aacgtgctgc
     3481 tggacaacga caggctggtc aagatcgggg actttggcct agccaaggcc gtgcccgaag
     3541 gccacgagta ctaccgcgtg cgcgaggatg gggacagccc cgtgttctgg tatgccccag
     3601 agtgcctgaa ggagtataag ttctactatg cgtcagatgt ctggtccttc ggggtgaccc
     3661 tgtatgagct gctgacgcac tgtgactcca gccagagccc ccccacgaaa ttccttgagc
     3721 tcataggcat tgctcagggt cagatgacag ttctgagact cactgagttg ctggaacgag
     3781 gggagaggct gccacggccc gacaaatgtc cctgtgaggt ctatcatctc atgaagaact
     3841 gctgggagac agaggcgtcc tttcgcccaa ccttcgagaa cctcataccc attctgaaga
     3901 cagtccatga gaagtaccaa ggccaggccc cttcagtgtt cagcgtgtgc ccccgggagg
     3961 gcccaccagg cccatacacc gacatcgaga tgaaccggct gggcaagtaa agatctaagg
     4021 taccaatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
     4081 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4141 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4201 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4261 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4321 ggaaagaacc agctggggct cgagggggga tccccacgcg ccctgtagcg gcgcattaag
     4381 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4441 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4501 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4561 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4621 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4681 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4741 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4801 gcttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc
     4861 aaccggggtg ggtaccgagc tcgaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4921 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4981 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5041 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5101 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5161 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5221 tgcaaaaagc tcccgggagc ttggatatcc attttcggat ctgatcaaga gacaggatga
     5281 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5341 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5401 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5461 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5521 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5581 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5641 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5701 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5761 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5821 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5881 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5941 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6001 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6061 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     6121 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6181 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6241 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6361 ccaaactcat caatgtatct tatcatgtct ggatccgtcg acctcgagag cttggcgtaa
     6421 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6481 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6541 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6601 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6661 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6721 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6781 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6841 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6901 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6961 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7021 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7081 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7141 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7201 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7261 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7321 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7381 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7441 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7501 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7561 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7621 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7681 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7741 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7801 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7861 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7921 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7981 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8041 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8101 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8161 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8221 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8281 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8341 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8401 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8461 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8521 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8581 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     8641 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     8701 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     8761 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     8821 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     8881 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     8941 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9001 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9061 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9121 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9181 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9241 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9301 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9361 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     9421 ccactgctta ctggcttatc ga
//
