LOCUS       sequence-275736-        8562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TYK2 protein missing the tyrosine kinase (TK) domain 
            (aa 1-895).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Velazquez L, Mogensen KE, Barbieri G, Fellous M, Uze G, Pellegrini S
  TITLE     Distinct domains of the protein tyrosine kinase tyk2 required for 
            binding of interferon-alpha/beta and for signal transduction.
  JOURNAL   J Biol Chem. 1995 Feb 17;270(7):3327-34. doi: 
            10.1074/jbc.270.7.3327.
  PUBMED    7531704
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1074/jbc.270.7";
            journalName: "J Biol Chem"; date: "1995-02-17- 17"; volume: "270"; 
            issue: "7"; pages: "3327-34"
COMMENT     Sequence Label: pRC/CMV-TYK2(delta)TK-VSV
FEATURES             Location/Qualifiers
     source          1..8562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3340..3372
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(3407..3425)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3408..3425)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3445..3462)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3451..3675
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3721..4234
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3808..3827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4018..4039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4257..4277)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4262..4591
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4442..4577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4658..5452
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4712..4731)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5322..5341
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5626..5760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5663..5682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5717..5736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5791..5807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5791..5807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5804..5826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5815..5831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5839..5845,5846..5863,5864..5869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5884..5905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6022..6039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6193..6781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6273..6292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6952..7743,7744..7812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7575..7594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7813..7917)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7992..8011)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8183..8562
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctt gtcgaggaac tgaaaaacca
      301 gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg atcccccccc
      361 ccccccccca agaagccgcg gggactggct gcgcttgaca ggctgcactt ggatgggagc
      421 acctggtgcc tcgggactgc tccgatgccc gggtctgtgc tgaatgtgta atatgcggaa
      481 ctatattgaa acattacaac catcttttga tggcaacacc ctgaggacct cccttttcca
      541 gatggggaaa ctgaggccca gaattgctaa gtggcttgct tgagttgaca cagggagctc
      601 caggactcac cctcagctga gccacctgcc gggagcatgc ctctgcgcca ctgggggatg
      661 gccaggggca gtaagcccgt tggggatgga gcccagccca tggctgccat gggaggcctg
      721 aaggtgcttc tgcactgggc tggtccaggc ggcggggagc cctgggtcac tttcagtgag
      781 tcatcgctga cagctgagga agtctgcatc cacattgcac ataaagttgg tatcactcct
      841 ccttgcttca atctctttgc cctcttcgat gctcaggccc aagtctggtt gcccccaaac
      901 cacatcctag agatccccag agatgcaagc ctgatgctat atttccgcat aaggttttat
      961 ttccggaact ggcatggcat gaatcctcgg gaaccggctg tgtaccgttg tgggccccca
     1021 ggaaccgagg catcctcaga tcagacagca caggggatgc aactcctgga cccagcctca
     1081 tttgagtacc tctttgagca gggcaagcat gagtttgtga atgacgtggc atcactgtgg
     1141 gagctgtcga ccgaggagga gatccaccac tttaagaatg agagcctggg catggccttt
     1201 ctgcacctct gtcacctcgc tctccgccat ggcatccccc tggaggaggt ggccaagaag
     1261 accagcttca aggactgcat cccgcgctcc ttccgccggc atatccggca gcacagcgcc
     1321 ctgacccggc tgcgccttcg gaacgtcttc cgcaggttcc tgcgggactt ccagccgggc
     1381 cgactctccc agcagatggt catggtcaaa tacctagcca cactcgagcg gctggcaccc
     1441 cgcttcggca cagagcgtgt gcccgtgtgc cacctgaggc tgctggccca ggccgagggg
     1501 gagccctgct acatccggga cagtggggtg gcccctacag accctggccc tgagtctgct
     1561 gctgggcccc caacccacga ggtgctggtg acaggcactg gtggcatcca gtggtggcca
     1621 gtagaggagg aggtgaacaa ggaggagggt tctagtggca gcagtggcag gaacccccaa
     1681 gccagcctgt ttgggaagaa ggccaaggct cacaaggcat tcggccagcc ggcagacagg
     1741 ccgcgggagc cactgtgggc ctacttctgt gacttccggg acatcaccca cgtggtgctg
     1801 aaagagcact gtgtcagcat ccaccggcag gacaacaagt gcctggagct gagcttgcct
     1861 tcccgggctg cggcgctgtc cttcgtgtcg ctggtggacg gctatttccg cctgacggcc
     1921 gactccagcc actacctgtg ccacgaggtg gctcccccac ggctggtgat gagcatccgg
     1981 gatgggatcc acggacccct gctggagcca tttgtgcagg ccaagctgcg gcccgaggac
     2041 ggcctgtacc tcattcactg gagcaccagc cacccctacc gcctgatcct cacagtggcc
     2101 cagcgtagcc aggcaccaga cggcatgcag agcttgcggc tccgaaagtt ccccattgag
     2161 cagcaggacg gggccttcgt gctggagggc tggggccggt ccttccccag cgttcgggaa
     2221 cttggggctg ccttgcaggg ctgcttgctg agggccgggg atgactgctt ctctctgcgt
     2281 cgctgttgcc tgccccaacc aggagaaacc tccaatctca tcatcatgcg gggggctcgg
     2341 gccagcccca ggacactcaa cctcagccag ctcagcttcc accgggttga ccagaaggag
     2401 atcacccagc tgtcccactt gggccagggc acaaggacca acgtgtatga gggccgcctg
     2461 cgagtggagg gcagcgggga ccctgaggag ggcaagatgg atgacgagga ccccctcgtg
     2521 cctggcaggg accgtgggca ggagctacga gtggtgctca aagtgctgga ccctagtcac
     2581 catgacatcg ccctggcctt ctacgagaca gccagcctca tgagccaggt ctcccacacg
     2641 cacctggcct tcgtgcatgg cgtctgtgtg cgcggccctg aaaatatcat ggtgacagag
     2701 tacgtggagc acggacccct ggatgtgtgg ctgcggaggg agcggggcca tgtgcccatg
     2761 gcttggaaga tggtggtggc ccagcagctg gccagcgccc tcagctacct ggagaacaag
     2821 aacctggttc atggtaatgt gtgtggccgg aacatcctgc tggcccggct ggggttggca
     2881 gagggcacca gccccttcat caagctgagt gatcctggcg tgggcctggg cgccctctcc
     2941 agggaggagc gggtggagag gatcccctgg ctggcccccg aatgcctacc aggtggggcc
     3001 aacagcctaa gcaccgccat ggacaagtgg gggtttggcg ccaccctcct ggagatctgc
     3061 tttgacggag aggcccctct gcagagccgc agtccctccg agaaggagca tttctaccag
     3121 aggcagcacc ggctgcccga gccctcctgc ccacagctgg ccacactcac cagccagtgt
     3181 ctgacctatg agccaaccca gaggccatca ttccgcacca tcctgcgtga cctcacccgg
     3241 ctgcagcccc acaatcttgc tgacgtcttg actgtgaacc cggactcacc ggcgtcggac
     3301 cctacggttt tccacaagcg ggagggccca ccaggcccat acaccgacat cgagatgaac
     3361 cggctgggca agtaaagatc taaggtacca atctagaggg ccctattcta tagtgtcacc
     3421 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3481 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3541 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3601 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3661 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgagg ggggatcccc
     3721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3841 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4081 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4141 acgcgaattt taacaaaata ttaacgctta caatttaaat atttgcttat acaatcttcc
     4201 tgtttttggg gcttttctga ttatcaaccg gggtgggtac cgagctcgaa ttctgtggaa
     4261 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4321 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4381 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4441 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4501 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4561 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgga tatccatttt
     4621 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4681 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4741 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4801 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     4861 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4921 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4981 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5041 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5101 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5161 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5221 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5281 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5341 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5401 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5461 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5521 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5581 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5641 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5701 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     5761 cgtcgacctc gagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5821 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5881 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5941 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6001 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6061 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6601 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7921 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7981 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     8041 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     8101 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     8161 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     8221 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8281 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8341 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8401 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8461 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8521 acttggcagt acatctacgt attagtcatc gctattacca tg
//
