LOCUS       sequence-275737-        9443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TYK2 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Li Z, Rotival M, Patin E, Michel F, Pellegrini S
  TITLE     Two common disease-associated TYK2 variants impact exon splicing and
            TYK2 dosage.
  JOURNAL   PLoS One. 2020 Jan 21;15(1):e0225289. doi: 
            10.1371/journal.pone.0225289. eCollection 2020.
  PUBMED    31961910
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-01-21"; volume: "15(1):e0225289. doi"; pages: " 
            10.1371/journal.pone.0225289. eCollection 2020"
COMMENT     Sequence Label: pRC/CMV-TYK2-VSV
FEATURES             Location/Qualifiers
     source          1..9443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3859..3891
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(3926..3944)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3927..3944)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3964..3981)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3970..4194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4240..4753
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4327..4346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4537..4558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4776..4796)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4781..5110
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4961..5096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5177..5971
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5231..5250)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5841..5860
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6145..6279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6182..6201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6236..6255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6310..6326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6310..6326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6323..6345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6334..6350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6358..6364,6365..6382,6383..6388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6403..6424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6541..6558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6712..7300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6792..6811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7471..8262,8263..8331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8094..8113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8332..8436)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8511..8530)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8702..9081
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9082..9285
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9236..9256
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9330..9349
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9330..9348
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccccaaga agccgcgggg actggctgcg cttgacaggc tgcacttgga tgggagcacc
       61 tggtgcctcg ggactgctcc gatgcccggg tctgtgctga atgtgtaata tgcggaacta
      121 tattgaaaca ttacaaccat cttttgatgg caacaccctg aggacctccc ttttccagat
      181 ggggaaactg aggcccagaa ttgctaagtg gcttgcttga gttgacacag ggagctccag
      241 gactcaccct cagctgagcc acctgccggg agcatgcctc tgcgccactg ggggatggcc
      301 aggggcagta agcccgttgg ggatggagcc cagcccatgg ctgccatggg aggcctgaag
      361 gtgcttctgc actgggctgg tccaggcggc ggggagccct gggtcacttt cagtgagtca
      421 tcgctgacag ctgaggaagt ctgcatccac attgcacata aagttggtat cactcctcct
      481 tgcttcaatc tctttgccct cttcgatgct caggcccaag tctggttgcc cccaaaccac
      541 atcctagaga tccccagaga tgcaagcctg atgctatatt tccgcataag gttttatttc
      601 cggaactggc atggcatgaa tcctcgggaa ccggctgtgt accgttgtgg gcccccagga
      661 accgaggcat cctcagatca gacagcacag gggatgcaac tcctggaccc agcctcattt
      721 gagtacctct ttgagcaggg caagcatgag tttgtgaatg acgtggcatc actgtgggag
      781 ctgtcgaccg aggaggagat ccaccacttt aagaatgaga gcctgggcat ggcctttctg
      841 cacctctgtc acctcgctct ccgccatggc atccccctgg aggaggtggc caagaagacc
      901 agcttcaagg actgcatccc gcgctccttc cgccggcata tccggcagca cagcgccctg
      961 acccggctgc gccttcggaa cgtcttccac aggttcctgc gggacttcca gccgggccga
     1021 ctctcccagc agatggtcat ggtcaaatac ctagccacac tcgagcggct ggcaccccgc
     1081 ttcggcacag agcgtgtgcc cgtgtgccac ctgaggctgc tggcccaggc cgagggggag
     1141 ccctgctaca tccgggacag tggggtggcc cctacagacc ctggccctga gtctgctgct
     1201 gggcccccaa cccacgaggt gctggtgaca ggcactggtg gcatccagtg gtggccagta
     1261 gaggaggagg tgaacaagga ggagggttct agtggcagca gtggcaggaa cccccaagcc
     1321 agcctgtttg ggaagaaggc caaggctcac aaggcagtcg gccagccggc agacaggccg
     1381 cgggagccac tgtgggccta cttctgtgac ttccgggaca tcacccacgt ggtgctgaaa
     1441 gagcactgtg tcagcatcca ccggcaggac aacaagtgcc tggagctgag cttgccttcc
     1501 cgggctgcgg cgctgtcctt cgtgtcgctg gtggacggct atttccgcct gacggccgac
     1561 tccagccact acctgtgcca cgaggtggct cccccacggc tggtgatgag catccgggat
     1621 gggatccacg gacccctgct ggagccattt gtgcaggcca agctgcggcc cgaggacggc
     1681 ctgtacctca ttcactggag caccagccac ccctaccgcc tgatcctcac agtggcccag
     1741 cgtagccagg caccagacgg catgcagagc ttgcggctcc gaaagttccc cattgagcag
     1801 caggacgggg ccttcgtgct ggagggctgg ggccggtcct tccccagcgt tcgggaactt
     1861 ggggctgcct tgcagggctg cttgctgagg gccggggatg actgcttctc tctgcgtcgc
     1921 tgttgcctgc cccaaccagg agaaacctcc aatctcatca tcatgcgggg ggctcgggcc
     1981 agccccagga cactcaacct cagccagctc agcttccacc gggttgacca gaaggagatc
     2041 acccagctgt cccacttggg ccagggcaca aggaccaacg tgtatgaggg ccgcctgcga
     2101 gtggagggca gcggggaccc tgaggagggc aagatggatg acgaggaccc cctcgtgcct
     2161 ggcagggacc gtgggcagga gctacgagtg gtgctcaaag tgctggaccc tagtcaccat
     2221 gacatcgccc tggccttcta cgagacagcc agcctcatga gccaggtctc ccacacgcac
     2281 ctggccttcg tgcatggcgt ctgtgtgcgc ggccctgaaa atatcatggt gacagagtac
     2341 gtggagcacg gacccctgga tgtgtggctg cggagggagc ggggccatgt gcccatggct
     2401 tggaagatgg tggtggccca gcagctggcc agcgccctca gctacctgga gaacaagaac
     2461 ctggttcatg gtaatgtgtg tggccggaac atcctgctgg cccggctggg gttggcagag
     2521 ggcaccagcc ccttcatcaa gctgagtgat cctggcgtgg gcctgggcgc cctctccagg
     2581 gaggagcggg tggagaggat cccctggctg gcccccgaat gcctaccagg tggggccaac
     2641 agcctaagca ccgccatgga caagtggggg tttggcgcca ccctcctgga gatctgcttt
     2701 gacggagagg cccctctgca gagccgcagt ccctccgaga aggagcattt ctaccagagg
     2761 cagcaccggc tgcccgagcc ctcctgccca cagctggcca cactcaccag ccagtgtctg
     2821 acctatgagc caacccagag gccatcattc cgcaccatcc tgcgtgacct cacccggctg
     2881 cagccccaca atcttgctga cgtcttgact gtgaacccgg actcaccggc gtcggaccct
     2941 acggttttcc acaagcgcta tttgaaaaag atccgagatc tgggcgaggg tcacttcggc
     3001 aaggtcagct tgtactgcta cgatccgacc aacgacggca ctggcgagat ggtggcggtg
     3061 aaagccctca aggcagactg cggcccccag caccgctcgg gctggaagca ggagattgac
     3121 attctgcgca cgctctacca cgagcacatc atcaagtaca agggctgctg cgaggaccaa
     3181 ggcgagaagt cgctgcagct ggtcatggag tacgtgcccc tgggcagcct ccgagactac
     3241 ctgccccggc acagcatcgg gctggcccag ctgctgctct tcgcccagca gatctgcgag
     3301 ggcatggcct atctgcacgc gcagcactac atccaccgag acctagccgc gcgcaacgtg
     3361 ctgctggaca acgacaggct ggtcaagatc ggggactttg gcctagccaa ggccgtgccc
     3421 gaaggccacg agtactaccg cgtgcgcgag gatggggaca gccccgtgtt ctggtatgcc
     3481 ccagagtgcc tgaaggagta taagttctac tatgcgtcag atgtctggtc cttcggggtg
     3541 accctgtatg agctgctgac gcactgtgac tccagccaga gcccccccac gaaattcctt
     3601 gagctcatag gcattgctca gggtcagatg acagttctga gactcactga gttgctggaa
     3661 cgaggggaga ggctgccacg gcccgacaaa tgtccctgtg aggtctatca tctcatgaag
     3721 aactgctggg agacagaggc gtcctttcgc ccaaccttcg agaacctcat acccattctg
     3781 aagacagtcc atgagaagta ccaaggccag gccccttcag tgttcagcgt gtgcccccgg
     3841 gagggcccac caggcccata caccgacatc gagatgaacc ggctgggcaa gtaaagatct
     3901 aaggtaccaa tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     3961 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4021 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4081 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4141 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4201 aggcggaaag aaccagctgg ggctcgaggg gggatcccca cgcgccctgt agcggcgcat
     4261 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4321 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4381 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4441 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4501 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4561 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4621 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4681 taacgcttac aatttaaata tttgcttata caatcttcct gtttttgggg cttttctgat
     4741 tatcaaccgg ggtgggtacc gagctcgaat tctgtggaat gtgtgtcagt tagggtgtgg
     4801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4861 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4921 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5041 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5101 cttttgcaaa aagctcccgg gagcttggat atccattttc ggatctgatc aagagacagg
     5161 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5221 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5281 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5341 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5401 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5461 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5521 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5581 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5641 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5701 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5761 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5821 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5881 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5941 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6001 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6241 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtcgacctcg agagcttggc
     6301 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6361 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6421 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6481 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7441 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7501 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7561 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7621 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7681 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7741 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7801 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7861 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7921 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7981 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8041 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8101 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8161 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8221 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8281 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8341 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8461 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     8521 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     8581 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     8641 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     8701 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     8761 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8821 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     8881 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     8941 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     9001 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     9061 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     9121 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     9181 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     9241 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     9301 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     9361 tgtcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     9421 tcaggtcccg gatccccccc ccc
//
