LOCUS       sequence-275738-       11298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline-inducible TEADi (GFP-tagged inhibitor of the 
            interaction of YAP1 and TAZ with TEAD transcription factors with 
            nuclear localization signal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Yuan Y, Park J, Feng A, Awasthi P, Wang Z, Chen Q, 
            Iglesias-Bartolome R
  TITLE     YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by 
            regulating cell proliferation and limiting KLF4 activity.
  JOURNAL   Nat Commun. 2020 Mar 19;11(1):1472. doi: 10.1038/s41467-020-15301-0.
  PUBMED    32193376
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-19"; volume: "11(1):1472. doi"; pages: " 
            10.1038/s41467-020-15301-0"
COMMENT     Sequence Label: pInducer20 EGFP-TEADi
FEATURES             Location/Qualifiers
     source          1..11298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(74..76,77..79,80..790)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGVK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(119..140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(380..399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     727..748
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1100..1120
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1150..1174)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1227..1253
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1339..2550
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2387..2406
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2609..3226,3227..3232,3233..3268,3269..3271,3272..3307,
                     3308..3310,3311..3346,3347..3355)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3407..3980
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3574..3591)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3801..3820
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3996..4790
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3996..4790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4050..4069)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4660..4679
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4967..5555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5020..5040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5438..5449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5630..5863
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5982..6000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6068..6172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6173..6241,6242..7033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6391..6410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7204..7792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7693..7712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7946..7963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8203..8224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8239..8244,8245..8262,8263..8269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8277..8293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8282..8304
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8301..8317
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8301..8317
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8367..8386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8578..9211
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9258..9383
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9880..10113
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10298..10342
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10635..10752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10807..10825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10849..10867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10891..10909
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10933..10951
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10996..11014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11038..11056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11080..11098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        11131..11169
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11166..11190
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac
       61 cagatctgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      121 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      181 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      241 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      301 cgaccacatg aagcagcacg acttctttaa gtccgccatg cccgaaggct acgtccagga
      361 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      421 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      481 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      541 caagcagaag aacggcgtca aggtgaactt caagatccgc cacaacatcg aggacggcag
      601 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      661 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      721 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      781 gctgtacaag tccggactca gatctggatc ctctgttgac gatcactttg caaaagcgtt
      841 gggcgacaca tggttgcaaa taggtgatcc tccggtcgcg acaaatccaa aaactgctaa
      901 cgtcccccag accgtgccta tgcgcctccg gaagttgcca gacagtttct ttaaggagcc
      961 ggaaggcgac cctcctgtgg caaccaatcc taaaccctcc tcatggagaa agaagattct
     1021 ccctgagtct ttcttcaagg aaccgggaga tcccccagtt gcaacaaagc gcacggcgga
     1081 tggtagcgaa tttgaatccc ctaaaaaaaa acgaaaggtt gaataagcgg ccgcactcga
     1141 gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     1201 cgagtctaga gggcccgcgg ttcgaatacc catacgacgt cccagactac gcttagtaat
     1261 gattaattaa actagaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     1321 gtaacccacc caagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     1381 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     1441 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     1501 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     1561 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     1621 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     1681 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     1741 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     1801 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     1861 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     1921 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     1981 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     2041 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     2101 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     2161 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     2221 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     2281 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     2341 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     2401 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     2461 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     2521 ttctggccgt ttttggcttt tttgttagac gaagcttggt accgagctcg gatctccacc
     2581 ccgtaccggt cctgcagtcg aattcaccat gtctagactg gacaagagca aagtcataaa
     2641 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     2701 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     2761 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     2821 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     2881 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     2941 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     3001 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     3061 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     3121 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     3181 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     3241 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     3301 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     3361 taaggatccg cggccgcact agaggaattc cgcccctctc cccccccccc tctccctccc
     3421 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     3481 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     3541 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     3601 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     3661 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     3721 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     3781 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     3841 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     3901 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     3961 tttgaaaaac acgatgataa gcttaccggt ccaccatgat tgaacaagat ggattgcacg
     4021 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4081 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4141 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     4201 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4261 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4321 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4381 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4441 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4501 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     4561 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4621 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4681 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4741 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga tgtacaagta
     4801 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     4861 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4921 gtttagtccc tcccaattcg atatcaagct tatcgcgata gatcctaatc aacctctgga
     4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5341 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     5581 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5641 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5701 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5761 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5821 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5881 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     5941 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     6001 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     6421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7801 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7861 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7921 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7981 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
     8041 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8101 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
     8161 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8221 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8281 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     8341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8401 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     8461 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     8521 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     8581 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     8641 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     8701 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     8761 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8821 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8881 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8941 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     9001 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     9061 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9121 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9181 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9241 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9301 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9361 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9421 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9481 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9541 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9601 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9661 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9721 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9781 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9841 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9901 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9961 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    10021 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    10081 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    10141 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    10201 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    10261 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    10321 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10381 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10441 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10501 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    10561 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
    10621 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10681 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10741 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac taggatcctt
    10801 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
    10861 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
    10921 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
    10981 tcagtgattt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
    11041 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
    11101 agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga ggcctatata
    11161 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
    11221 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt
    11281 ggaattctgc agatatca
//
