LOCUS       sequence-275739-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of kinase-dead TYK2-K930R missing the two tyrosines at 
            the activation loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Gauzzi MC, Velazquez L, McKendry R, Mogensen KE, Fellous M, 
            Pellegrini S
  TITLE     Interferon-alpha-dependent activation of Tyk2 requires 
            phosphorylation of positive regulatory tyrosines by another kinase.
  JOURNAL   J Biol Chem. 1996 Aug 23;271(34):20494-500. doi: 
            10.1074/jbc.271.34.20494.
  PUBMED    8702790
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.271.34"; journalName: "J Biol Chem"; date: "1996-08-23-
            23"; volume: "271"; issue: "34"; pages: "20494-500"
COMMENT     Sequence Label: pRC/CMV-TYK2K930R-FF-VSV
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4257..4273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4257..4273)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(4283..4301)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4284..4301)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4321..4338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4327..4551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4597..5110
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4684..4703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4894..4915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5133..5153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5138..5467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5318..5453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5534..6328
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5588..5607)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6198..6217
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6502..6636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6539..6558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6593..6612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6667..6683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6667..6683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6680..6702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6691..6707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6715..6721,6722..6739,6740..6745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6760..6781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6898..6915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7069..7657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7149..7168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7828..8619,8620..8688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8451..8470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8689..8793)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8868..8887)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9059..9438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctt gtcgaggaac tgaaaaacca
      301 gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg atcccccccc
      361 ccccccccaa gaagccgcgg ggactggctg cgcttgacag gctgcacttg gatgggagca
      421 cctggtgcct cgggactgct ccgatgcccg ggtctgtgct gaatgtgtaa tatgcggaac
      481 tatattgaaa cattacaacc atcttttgat ggcaacaccc tgaggacctc ccttttccag
      541 atggggaaac tgaggcccag aattgctaag tggcttgctt gagttgacac agggagctcc
      601 aggactcacc ctcagctgag ccacctgccg ggagcatgcc tctgcgccac tgggggatgg
      661 ccaggggcag taagcccgtt ggggatggag cccagcccat ggctgccatg ggaggcctga
      721 aggtgcttct gcactgggct ggtccaggcg gcggggagcc ctgggtcact ttcagtgagt
      781 catcgctgac agctgaggaa gtctgcatcc acattgcaca taaagttggt atcactcctc
      841 cttgcttcaa tctctttgcc ctcttcgatg ctcaggccca agtctggttg cccccaaacc
      901 acatcctaga gatccccaga gatgcaagcc tgatgctata tttccgcata aggttttatt
      961 tccggaactg gcatggcatg aatcctcggg aaccggctgt gtaccgttgt gggcccccag
     1021 gaaccgaggc atcctcagat cagacagcac aggggatgca actcctggac ccagcctcat
     1081 ttgagtacct ctttgagcag ggcaagcatg agtttgtgaa tgacgtggca tcactgtggg
     1141 agctgtcgac cgaggaggag atccaccact ttaagaatga gagcctgggc atggcctttc
     1201 tgcacctctg tcacctcgct ctccgccatg gcatccccct ggaggaggtg gccaagaaga
     1261 ccagcttcaa ggactgcatc ccgcgctcct tccgccggca tatccggcag cacagcgccc
     1321 tgacccggct gcgccttcgg aacgtcttcc gcaggttcct gcgggacttc cagccgggcc
     1381 gactctccca gcagatggtc atggtcaaat acctagccac actcgagcgg ctggcacccc
     1441 gcttcggcac agagcgtgtg cccgtgtgcc acctgaggct gctggcccag gccgaggggg
     1501 agccctgcta catccgggac agtggggtgg cccctacaga ccctggccct gagtctgctg
     1561 ctgggccccc aacccacgag gtgctggtga caggcactgg tggcatccag tggtggccag
     1621 tagaggagga ggtgaacaag gaggagggtt ctagtggcag cagtggcagg aacccccaag
     1681 ccagcctgtt tgggaagaag gccaaggctc acaaggcatt cggccagccg gcagacaggc
     1741 cgcgggagcc actgtgggcc tacttctgtg acttccggga catcacccac gtggtgctga
     1801 aagagcactg tgtcagcatc caccggcagg acaacaagtg cctggagctg agcttgcctt
     1861 cccgggctgc ggcgctgtcc ttcgtgtcgc tggtggacgg ctatttccgc ctgacggccg
     1921 actccagcca ctacctgtgc cacgaggtgg ctcccccacg gctggtgatg agcatccggg
     1981 atgggatcca cggacccctg ctggagccat ttgtgcaggc caagctgcgg cccgaggacg
     2041 gcctgtacct cattcactgg agcaccagcc acccctaccg cctgatcctc acagtggccc
     2101 agcgtagcca ggcaccagac ggcatgcaga gcttgcggct ccgaaagttc cccattgagc
     2161 agcaggacgg ggccttcgtg ctggagggct ggggccggtc cttccccagc gttcgggaac
     2221 ttggggctgc cttgcagggc tgcttgctga gggccgggga tgactgcttc tctctgcgtc
     2281 gctgttgcct gccccaacca ggagaaacct ccaatctcat catcatgcgg ggggctcggg
     2341 ccagccccag gacactcaac ctcagccagc tcagcttcca ccgggttgac cagaaggaga
     2401 tcacccagct gtcccacttg ggccagggca caaggaccaa cgtgtatgag ggccgcctgc
     2461 gagtggaggg cagcggggac cctgaggagg gcaagatgga tgacgaggac cccctcgtgc
     2521 ctggcaggga ccgtgggcag gagctacgag tggtgctcaa agtgctggac cctagtcacc
     2581 atgacatcgc cctggccttc tacgagacag ccagcctcat gagccaggtc tcccacacgc
     2641 acctggcctt cgtgcatggc gtctgtgtgc gcggccctga aaatatcatg gtgacagagt
     2701 acgtggagca cggacccctg gatgtgtggc tgcggaggga gcggggccat gtgcccatgg
     2761 cttggaagat ggtggtggcc cagcagctgg ccagcgccct cagctacctg gagaacaaga
     2821 acctggttca tggtaatgtg tgtggccgga acatcctgct ggcccggctg gggttggcag
     2881 agggcaccag ccccttcatc aagctgagtg atcctggcgt gggcctgggc gccctctcca
     2941 gggaggagcg ggtggagagg atcccctggc tggcccccga atgcctacca ggtggggcca
     3001 acagcctaag caccgccatg gacaagtggg ggtttggcgc caccctcctg gagatctgct
     3061 ttgacggaga ggcccctctg cagagccgca gtccctccga gaaggagcat ttctaccaga
     3121 ggcagcaccg gctgcccgag ccctcctgcc cacagctggc cacactcacc agccagtgtc
     3181 tgacctatga gccaacccag aggccatcat tccgcaccat cctgcgtgac ctcacccggc
     3241 tgcagcccca caatcttgct gacgtcttga ctgtgaaccc ggactcaccg gcgtcggacc
     3301 ctacggtttt ccacaagcgc tatttgaaaa agatccgaga tctgggcgag ggtcacttcg
     3361 gcaaggtcag cttgtactgc tacgatccga ccaacgacgg cactggcgag atggtggcgg
     3421 tgagagccct caaggcagac tgcggccccc agcaccgctc gggctggaag caggagattg
     3481 acattctgcg cacgctctac cacgagcaca tcatcaagta caagggctgc tgcgaggacc
     3541 aaggcgagaa gtcgctgcag ctggtcatgg agtacgtgcc cctgggcagc ctccgagact
     3601 acctgccccg gcacagcatc gggctggccc agctgctgct cttcgcccag cagatctgcg
     3661 agggcatggc ctatctgcac gcgcagcact acatccaccg agacctagcc gcgcgcaacg
     3721 tgctgctgga caacgacagg ctggtcaaga tcggggactt tggcctagcc aaggccgtgc
     3781 ccgaaggcca cgagttcttc cgcgtgcgcg aggatgggga cagccccgtg ttctggtatg
     3841 ccccagagtg cctgaaggag tataagttct actatgcgtc agatgtctgg tccttcgggg
     3901 tgaccctgta tgagctgctg acgcactgtg actccagcca gagccccccc acgaaattcc
     3961 ttgagctcat aggcattgct cagggtcaga tgacagttct gagactcact gagttgctgg
     4021 aacgagggga gaggctgcca cggcccgaca aatgtccctg tgaggtctat catctcatga
     4081 agaactgctg ggagacagag gcgtcctttc gcccaacctt cgagaacctc atacccattc
     4141 tgaagacagt ccatgagaag taccaaggcc aggccccttc agtgttcagc gtgtgctgag
     4201 gcacaatggc agccctgcct gggaggactg gaccaggcag tggctgcagc ccgggggatc
     4261 cactagttct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     4321 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4381 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4441 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4501 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4561 cggaaagaac cagctggggc tcgagggggg atccccacgc gccctgtagc ggcgcattaa
     4621 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4681 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4741 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4801 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4861 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4921 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5041 cgcttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
     5101 caaccggggt gggtaccgag ctcgaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5221 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5281 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5401 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5461 ttgcaaaaag ctcccgggag cttggatatc cattttcgga tctgatcaag agacaggatg
     5521 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5581 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5641 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5701 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5761 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5821 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5881 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5941 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6001 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6061 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6121 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6181 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6241 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6301 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6361 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6421 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6481 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6541 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6601 tccaaactca tcaatgtatc ttatcatgtc tggatccgtc gacctcgaga gcttggcgta
     6661 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6721 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6781 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8341 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8701 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8761 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8821 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     8881 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     8941 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     9001 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     9061 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     9121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     9181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9421 gtcatcgcta ttaccatg
//
