LOCUS       sequence-275747-        9788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Tei R, Baskin JM
  TITLE     Spatiotemporal control of phosphatidic acid signaling with 
            optogenetic, engineered phospholipase Ds.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 
            10.1083/jcb.201907013.
  PUBMED    31999306
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133675. doi"; 
            pages: " 10.1083/jcb.201907013"
COMMENT     Sequence Label: Endoplasmic reticulum-targeted optoPLD
FEATURES             Location/Qualifiers
     source          1..9788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             761..2254
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2288..2992
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2430..2448)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2698..2717
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4607..4663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4670..5179
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     complement(5506..5523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5512..5736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5869..5888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6020..6067
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6075..7097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6075..7097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7227..7348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7264..7283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7318..7337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7397..7413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7397..7413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7410..7432)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7421..7437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7445..7451,7452..7469,7470..7475))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7490..7511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7628..7645)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7799..8387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7879..7898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8558..9349,9350..9418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9181..9200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9419..9523)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9598..9617)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtacgccacc atgaagatgg acaaaaagac
      781 tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc
      841 tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta
      901 tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt
      961 gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt
     1021 ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt
     1081 ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca
     1141 aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc
     1201 ttttacgagt ttcaattctt actggaggaa atgcttagat atgtcgattg aatccgttat
     1261 gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg
     1321 ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac
     1381 tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa
     1441 gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact
     1501 ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat
     1561 gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt
     1621 tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac
     1681 tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa
     1741 gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga
     1801 gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt
     1861 gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga
     1921 tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg
     1981 ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg
     2041 tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca
     2101 tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta
     2161 tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag
     2221 aacccgtgaa gcacagatca tgatcggagc agcaggtgga ggcggttcag gcggaggtgg
     2281 cagcggcgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2341 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2401 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2461 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2521 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2581 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2641 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2701 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2761 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2821 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2881 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2941 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agagatctcg
     3001 aagcgcggcc gcgggagcag gaggagcagc tcgaggcgca gcagcgggag caggaggagc
     3061 aggtcgatcg ggcggatctg cagattcagc tacaccacat ttggacgccg ttgagcaaac
     3121 attgaggcaa gtgtccccag gcctggaggg cgatgtatgg gaaagaacta gtggaaataa
     3181 attggacgga agtgccgctg atcccagtga ttggttgttg caaaccccag gatgttgggg
     3241 cgatgataaa tgcgctgaca gagttgggac aaaaaggttg ctggctaaga tgactgaaaa
     3301 tatagggaat gccaccagaa ccgtagatat tagtacactt gcacctttcc caaacggcgc
     3361 attccaagac gctattgtcg ctggtttgaa ggagtccgca gctaaaggca ataagttgaa
     3421 agttaggata ctggttgggg ctgcacccgt ctaccacatg aatgtaatac cttccaagta
     3481 tagggacgaa ttgactgcaa aacttggcaa agccgcagaa aacataactt tgaacgtggc
     3541 tagtatgact acatcaaaaa cagctttctc ctggaaccat agcaaaatct tggttgtaga
     3601 cgggcaaagt gcccttactg gtggaataaa tagctggaag gatgattact tggatactac
     3661 tcatccagtg tctgatgttg atcttgcact gaccggacca gctgcaggct ctgccggtag
     3721 ataccttgac actttgtgga catggacctg ccaaaacaag agtaacatcg cttccgtctg
     3781 gtttgcagct tccggcaacg cagggtgcat gcccactatg cataaagata caaaccccaa
     3841 ggcatcacct gccaccggga atgtcccagt aatagccgtt ggagggttgg gcgttggcat
     3901 caaagatgtg gaccccaagt ccactttcag gcccgatctg ccaacagcta gcgatactaa
     3961 gtgtgtggta ggacttcacg acaatactaa cgccgataga gactatgata cagtcaaccc
     4021 agaagagagt gccttgagag ctcttgttgc ttcagcaaaa ggtcacattg aaatatctca
     4081 gcaggatttg aacgctacat gtccaccctt gcctaggtat gacattagat tgtacgatgc
     4141 cttggctgct aaaatggctg ctggggttaa ggtgagaatt gtggttagcg acccagccaa
     4201 caggggcgca gtgggcagtg ggggttacag ccaaataaaa tcacttagtg aaatatcaga
     4261 cactttgaga aacaggcttg caaatatcac aggcggtcaa caagctgcca aaactgcaat
     4321 gtgtagcaat ttgcagcttg caacctttag atcctccccc aacggcaaat gggcagacgg
     4381 gcatccttat gcccaacacc ataagttggt ttccgtcgac tcatccacat tttatatcgg
     4441 ttcaaaaaat ttgtacccat cctggctgca agattttggc tacatagtgg aatccccaga
     4501 ggctgccaag caactggatg ccaaattgct tgaccctcag tggaagtact cacaggagac
     4561 tgcaacagtg gattacgcaa gaggaatctg caacgctgga agcggcgcta caaactttag
     4621 tctgctcaag caggctggcg atgtcgagga aaacccaggt cccggtacca tgaatggagc
     4681 tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc
     4741 tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc
     4801 ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc
     4861 gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac
     4921 gacgcttggg actggaaatt tcaaggcagc gaagtttgat acagagacta aggattgtaa
     4981 tgaggcggcg aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga
     5041 gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca
     5101 caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt
     5161 ggagaaaacg gattatattc atgtgaccgg tggcgccttc ctggctttgc ctgttatcat
     5221 ggttgttgcc ttttcaatgt gcattatctg tttgcttatg gctggtgaca cttggacaga
     5281 aacactggcc tatgtgctct tctggggagt tgcaagcatt ggaacatttt ttatcattct
     5341 ttacaatggc aaagattttg tcgatgctcc cagactggtc cagaaagaaa agatagactg
     5401 agtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt
     5461 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     5521 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5581 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5641 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5701 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5761 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5821 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5881 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5941 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6001 gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta gaaagtatag
     6061 gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     6121 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     6181 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     6241 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     6301 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     6361 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     6421 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     6481 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     6541 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     6601 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     6661 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     6721 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     6781 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     6841 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     6901 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     6961 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     7021 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     7081 cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc ttctatgaaa
     7141 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7201 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7321 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7381 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7441 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7501 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7561 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7621 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7681 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7741 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7801 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7861 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7921 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7981 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8041 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8101 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8161 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8221 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8281 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8341 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8401 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8461 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8521 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8581 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8641 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8701 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8761 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8821 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8881 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8941 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9001 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9061 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9121 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9181 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9241 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9301 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9361 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9421 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9481 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9541 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     9601 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     9661 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     9721 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     9781 tacgcgtt
//
