LOCUS       sequence-275754-        9993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  neomycin resistance; constitutive promoter driving reverse tet 
            activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol. 2015 Jan 29."
COMMENT     Sequence Label: AAVS1-SA-2A-NEO-CAG-RTTA3
FEATURES             Location/Qualifiers
     source          1..9993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1758
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1790..1807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1796..2020
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2026..2405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2679
                     /label=chicken beta-actin promoter
     intron          2680..3696
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3620..3642
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3704..3723
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3763..4470
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     primer_bind     complement(4560..4579)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4625..4680
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4679..4698)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5041..5057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5065..5081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5089..5095,5096..5113,5114..5119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5134..5155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5229..6065
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(6099..6118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6100..6118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6125..6142)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6125..6141)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6134..6156)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6279..6581
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6460..6479
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(7978..8769,8770..8838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8601..8620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8962..9550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9451..9470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9704..9721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9838..9859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9874..9879,9880..9897,9898..9904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9912..9928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9936..9952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9973..9991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatggga
      961 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     1021 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     1081 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     1141 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     1201 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     1261 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     1321 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     1381 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     1441 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     1501 gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     1561 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     1621 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     1681 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     1741 cttgacgagt tcttctgatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     1801 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     1861 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     1921 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     1981 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg gtctcgacat tgattattga
     2041 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2101 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2161 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2221 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2281 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2341 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2401 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     2461 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     2521 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     2581 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     2641 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     2701 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     2761 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     2821 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     2881 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     2941 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     3001 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     3061 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     3121 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     3181 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     3241 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     3301 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3361 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     3421 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     3481 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     3541 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     3601 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     3661 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     3721 gtgctgtctc atcattttgg caaagaattc cttaaggcca ccatgtctag gctggacaag
     3781 agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg
     3841 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     3901 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     3961 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
     4021 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     4081 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     4141 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     4201 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     4261 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     4321 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     4381 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     4441 gattttgacc ttgacatgct ccccgggtaa acgcgtgaat tcactcctca ggtgcaggct
     4501 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     4561 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     4621 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     4681 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     4741 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     4801 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     4861 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     4921 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     4981 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     5041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcgacagtac
     5221 taagctttac tagggacagg attggtgaca gaaaagcccc atccttaggc ctcctccttc
     5281 ctagtctcct gatattgggt ctaaccccca cctcctgtta ggcagattcc ttatctggtg
     5341 acacaccccc atttcctgga gccatctctc tccttgccag aacctctaag gtttgcttac
     5401 gatggagcca gagaggatcc tgggagggag agcttggcag ggggtgggag ggaagggggg
     5461 gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct accctctccc agaacctgag
     5521 ctgctctgac gcggctgtct ggtgcgtttc actgatcctg gtgctgcagc ttccttacac
     5581 ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa taagttggtc ctgagttcta
     5641 actttggctc ttcacctttc tagtccccaa tttatattgt tcctccgtgc gtcagtttta
     5701 cctgtgagat aaggccagta gccagccccg tcctggcagg gctgtggtga ggaggggggt
     5761 gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg
     5821 ccgcctctac tccctttctc tttctccatc cttctttcct taaagagtcc ccagtgctat
     5881 ctgggacata ttcctccgcc cagagcaggg tcccgcttcc ctaaggccct gctctgggct
     5941 tctgggtttg agtccttggc aagcccagga gaggcgctca ggcttccctg tcccccttcc
     6001 tcgtccacca tctcatgccc ctggctctcc tgccccttcc ctacaggggt tcctggctct
     6061 gctctaaggg cgaattcgcg gccgctaaat tcaattcgcc ctatagtgag tcgtattaca
     6121 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6181 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6241 atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt tacacctata
     6301 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgccgg
     6361 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     6421 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     6481 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     6541 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc atgagattat
     6601 caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct
     6661 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     6721 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
     6781 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     6841 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagctctg
     6901 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6961 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7021 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7081 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     7141 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7201 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7261 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7321 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7381 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7441 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catgcatggc
     7501 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7561 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7621 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7681 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tattaacgct
     7741 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc
     7801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7861 ttcaaatatg tatccgctca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8521 gactggtgaa tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8881 ttgtctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9721 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9781 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9841 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9901 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9961 cgccaagctc agaattaacc ctcactaaag gga
//
