LOCUS       sequence-275761-        8673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knocking down of human HNF4-alpha through shRNA and amiRNA targeting
            HNF4-alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Pan G, Cavalli M, Carlsson B, Skrtic S, Kumar C, Wadelius C
  TITLE     rs953413 Regulates Polyunsaturated Fatty Acid Metabolism by 
            Modulating ELOVL2 Expression.
  JOURNAL   iScience. 2020 Jan 9:100808. doi: 10.1016/j.isci.2019.100808.
  PUBMED    31928966
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience. 
            2020 Jan 9:100808. doi: 10.1016/j.isci.2019.100808."
COMMENT     Sequence Label: pBMN-AS-HNF4-alpha
FEATURES             Location/Qualifiers
     source          1..8673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1935..1955
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2056..2065
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2086..2748
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             2830..2883
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2899..2955
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2977..3042
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3055..3654
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3055..3074)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3551..3571
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4216..4804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4269..4289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4687..4698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4916..5130
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5051..5070
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     LTR             5235..5415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5487..5608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5524..5543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5578..5597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5648..5783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5816..5832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5816..5832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5829..5851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5840..5856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5864..5870,5871..5888,5889..5894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5909..5930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6047..6064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6218..6806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6977..7768,7769..7837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7600..7619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7838..7942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8010..8028
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8066..8088)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8188..8207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8401..8423
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8415..8432
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8416..8432
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8447..8673
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggtt tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg ggatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatact agtattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagattc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     2101 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     2161 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     2221 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     2281 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     2341 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     2401 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     2461 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     2521 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     2581 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     2641 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     2701 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     2761 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     2821 ggctccgggg agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggg
     2881 cccgctagcg gatcaggtgc cacgaacttc tctctgttaa agcaagcagg agacgtggaa
     2941 gaaaaccccg gtcccttaat taatggctcc ggaccggtga aacagacttt gaattttgac
     3001 cttctcaagt tggcgggaga cgtggagtcc aacccaggcc cagtcgaccc cgggatgacc
     3061 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     3121 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     3181 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     3241 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     3301 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     3361 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     3421 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3481 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3541 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3601 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatatcgt
     3661 cgctgttatc tcattgtctg tgtgattggg ggagctgcgg cggggaggat gctgtggtcc
     3721 cttcctccgg cgttccccac ccccatccct ctccccgctg tcagtgcgca cgcacacgcg
     3781 ccgcttttta tttctttttc ctggttttct tattccatct tctacccacc cctcttcctt
     3841 tctttcacct ttccttcctt ccttcctcct ttccttcctc aggagaaagg ccgatatcta
     3901 cgcactcgac ggtatagcta ttttaaatgt ccatacaaat agcttgacct tcgagtgctg
     3961 agaattcggc tggctgagca ccgtgggtcg gcgagggccc gccaaggaag gagcgaccga
     4021 ccgagccagg cgccctccgc agacctccgc gcagcggccg cgggcgcgag gggaggggtc
     4081 tggagctccc tccggctgcc tgtcccgcac cggagcccgt ggggtgggga ggtgtgcagc
     4141 ctgtgacaga caggggctta gagatgcaaa cagactcagg gagagaaaca gaagctgatt
     4201 ctgtgacaga agcagaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4261 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4321 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4381 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4441 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4501 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4561 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4621 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4681 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4741 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4801 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4861 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgatgtcaag aattggaacg
     4921 ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc gggaacaccc
     4981 agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg cgccctgcaa
     5041 tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt
     5101 gggaatctta taagttctgt atgagaccac ttggatccga accacatgta ctcctgcaga
     5161 ttcttcctgt cagaaatctg caggagtaca tgtggttctt tttgaattct tcgattctgc
     5221 tttttgcttc tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5281 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5341 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt cagtcagtgt
     5401 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5461 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5521 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5581 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5701 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5761 gtgaggaggc ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat
     5821 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5881 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5941 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6001 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7981 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     8041 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8101 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8161 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8221 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     8281 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8341 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8401 cccagtcacg acgttgtaaa acgacggcca gtgccaagct gacgcgtgta gtcttatgca
     8461 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     8521 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     8581 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     8641 attgtattta agtgcctagc tcgatacata aac
//
