LOCUS       sequence-275763-        3827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Npga.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P195
FEATURES             Location/Qualifiers
     source          1..3827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..429
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     372..392
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1580..1780
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        1855..1959
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3027..3615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3516..3535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3769..3786
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atggaggtca acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca
       61 gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc
      121 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt
      181 ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc
      241 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt
      301 ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt aagggatgac
      361 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
      421 gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca acaacaaaca
      481 acaaacaaca ttacaattac atttacaatt atcgatacaa tggtgcagga cacttcaagt
      541 gctagcacaa gtccaatttt aaccagatgg tatatagaca ctcgacctct cacggcaagt
      601 accgccgcac ttccgctgct ggaaacgctg caaccagctg atcagatctc cgtccaaaaa
      661 tattatcatc tcaaggacaa gcacatgtca cttgcctcca accttttgaa gtatttattc
      721 gttcatcgaa attgccgaat accatggagt agtattgtca tatcaagaac gcccgacccg
      781 cacagaaggc cttgctatat acctccttca ggttcacaag aggactcctt caaagacgga
      841 tatacgggaa tcaacgtaga attcaacgtt agtcaccaag ccagcatggt ggcaatcgca
      901 ggtacggctt tcacacctaa tagcggtgga gatagcaaac tcaaaccgga ggtcggaata
      961 gatataacat gtgtaaatga gaggcagggc aggaatggag aagaaagatc cttggaatcc
     1021 ctgaggcagt atatagatat attcagcgaa gtattcagca ccgcagagat ggctaatata
     1081 aggcgacttg atggggtttc ctcaagttcc cttagtgcag acagactcgt agattatgga
     1141 taccgattat tctacaccta ttgggcctta aaagaggctt atatcaagat gacaggtgag
     1201 gccttattgg ctccttggtt gagagagctg gagttttcca acgtggtcgc tccagccgct
     1261 gtcgccgaaa gcggggactc tgctggagac tttggcgaac cgtacactgg cgtgcgtaca
     1321 acattgtata agaacctcgt agaggacgta cgaattgagg tagctgcttt gggaggagat
     1381 tatctgttcg caacagccgc tagaggtggt ggtataggcg catcctcacg tcctggtggt
     1441 ggcccagatg gctctggaat tagatcccag gacccttgga ggccgttcaa gaaactcgat
     1501 atagagcgtg acatacagcc atgtgctacg ggcgtttgta attgcctgtc ctcaggatag
     1561 gcttgatccc ccgtcgacag ctagctatat catcaattta tgtattacac ataatatcgc
     1621 actcagtctt tcatctacgg caatgtacca gctgatataa tcagttattg aaatatttct
     1681 gaatttaaac ttgcatcaat aaatttatgt ttttgcttgg actataatac ctgacttgtt
     1741 attttatcaa taaatattta aactatattt ctttcaagat cgctgttaga gctcggacgt
     1801 gggcaggtgt gatgaaaagc cttagggtca ggtggcactt ttcggggaaa tgtgcgatga
     1861 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctaat gagacaataa
     1921 ccctgataaa tgcttcaatt atattgaaaa aggaagagta tgagtatcca acatttccgt
     1981 gtcgccctta ttcccttttt tgcggccttc tgccttcctg tttttgctca cccagaaacg
     2041 ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc gagtgggtta cattgaactg
     2101 gatctcaaca gcggcaaaat cttagagagt tttcgccccg aagaacgctt cccaatgatg
     2161 agcactttca aagttctgct atgtggcgcg gtgttatccc gtattgatgc tgggcaagag
     2221 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc accagtcaca
     2281 gaaaagcatc ttacggatgg catgacagta agagaattat gtagtgctgc cataaccatg
     2341 agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa ggagctaacc
     2401 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2461 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaact
     2521 ctccgcaaac tattaactgg cgaactactt actcttgctt cacgccagca actcattgac
     2581 tggatggagg cggacaaagt tgcaggacca cttctgcgct cggcacttcc ggctggctgg
     2641 tttattgctg ataaatctgg agccggtgag cgtggctctc gcggtatcat tgcagcactg
     2701 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2761 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2821 ctgtcagacg aagggaaata aatagtagcc cgcctgatgt gcgggctttt ttttggtggt
     2881 ggttaccagt gtcgcttagc taccgtaggt cctcttaggc ggtccgatta gcgacatagt
     2941 catcaatgcc ttgacggcaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacta
     3121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3361 tcacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3781 agcgaggaag cggaagagcg cccacctgct cattgacggc gcgccct
//
