LOCUS       sequence-275766-        8334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: PTPRA_C723S-GFP fusion
FEATURES             Location/Qualifiers
     source          1..8334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3215..3239)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3247..3256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3253..3255,3256..3258,3259..3969)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3253..3255,3256..3258,3259..3969)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3298..3319)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3559..3578)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3906..3927
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4052..4069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4058..4282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4415..4434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4566..4613
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4621..5643
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4621..5643
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5773..5894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5810..5829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5864..5883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5943..5959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5943..5959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5956..5978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5967..5983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5991..5997,5998..6015,6016..6021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6036..6057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6174..6191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6345..6933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6425..6444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7104..7895,7896..7964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7727..7746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7965..8069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8144..8163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc gacaagtttg tacaaaaaag caggctccac catggattcc
      841 tggttcattc ttgttctgct cggcagtggt ctgatatgtg tcagtgccaa caatgctacc
      901 acagttgcac cttctgtagg aattacaaga ttaattaact catcaacggc agaaccagtt
      961 aaagaagagg ccaaaacttc aaatccaact tcttcactaa cttctctttc tgtggcacca
     1021 acattcagcc caaatataac tctgggaccc acctatttaa ccactgtcaa ttcttcagac
     1081 tctgacaatg ggaccacaag aacagcaagc accaattcta taggcattac aatttcacca
     1141 aatggaacgt ggcttccaga taaccagttc acggatgcca gaacagaacc ctgggagggg
     1201 aattccagca ccgcagcaac cactccagaa actttccctc cttcagatga gacaccaatt
     1261 attgcggtga tggtggccct gtcctctctg ctagtgatcg tgtttattat catagttttg
     1321 tacatgttaa ggtttaagaa atacaagcaa gctgggagcc attccaattc tttccgctta
     1381 tccaacggcc gcactgagga tgtggagccc cagagtgtgc cacttctggc cagatcccca
     1441 agcaccaaca ggaaataccc acccctgccc gtggacaagc tggaagagga aattaaccgg
     1501 agaatggcag acgacaataa gctcttcagg gaggaattca acgctctccc tgcatgtcct
     1561 atccaggcca cctgtgaggc tgcttccaag gaggaaaaca aggaaaaaaa tcgatatgta
     1621 aacatcttgc cttatgacca ctctagagtc cacctgacac cggttgaagg ggttccagat
     1681 tctgattaca tcaatgcttc attcatcaac ggttaccaag aaaagaacaa attcattgct
     1741 gcacaaggac caaaagaaga aacggtgaat gatttctggc ggatgatctg ggaacaaaac
     1801 acagccacca tcgtcatggt taccaacctg aaggagagaa aggagtgcaa gtgcgcccag
     1861 tactggccag accaaggctg ctggacctat gggaatattc gggtgtctgt agaggatgtg
     1921 actgtcctgg tggactacac agtacggaag ttctgcatcc agcaggtggg cgacatgacc
     1981 aacagaaagc cacagcgcct catcactcag ttccacttta ccagctggcc agactttggg
     2041 gtgcctttta ccccgatcgg catgctcaag ttcctcaaga aggtgaaggc ctgtaaccct
     2101 cagtatgcag gggccatcgt ggtccactgc agtgcaggtg tagggcgtac aggtaccttt
     2161 gtcgtcattg atgccatgct ggacatgatg catacagaac ggaaggtgga catgtatggc
     2221 tttgtgagcc ggatccgggc acagcgctgc cagatggtgc aaaccgatat gcagtatgtc
     2281 ttcatatacc aagcccttct ggagcattat ctctatggag atacagaact ggaagtgacc
     2341 tctctagaaa cccacctgca gaaaatttac aacaaaatcc cagggaccag caacaatgga
     2401 ttagaggagg agtttaagaa gttaacatca atcaaaatcc agaatgacaa gatgcggact
     2461 ggaaaccttc cagccaacat gaagaagaac cgtgttttac agatcattcc atatgaattc
     2521 aacagagtga tcattccagt taagcggggc gaagagaata cagactatgt gaacgcatcc
     2581 tttattgatg gctaccggca gaaggactcc tatatcgcca gccagggccc tcttctccac
     2641 acaattgagg acttctggcg aatgatctgg gagtggaaat cctgctctat cgtgatgcta
     2701 acagaactgg aggagagagg ccaggagaag tgtgcccagt actggccatc tgatggactg
     2761 gtgtcctatg gagatattac agtggaactg aagaaggagg aggaatgtga gagctacacc
     2821 gtccgagacc tcctggtcac caacaccagg gagaataaga gccggcagat ccggcagttc
     2881 cacttccatg gctggcctga agtgggcatc cccagtgacg gaaagggcat gatcagcatc
     2941 atcgccgccg tgcagaagca gcagcagcag tcagggaacc accccatcac cgtgcactcc
     3001 agcgccgggg caggaaggac ggggaccttc tgtgccctga gcaccgtcct ggagcgtgtg
     3061 aaagcagagg ggattttgga tgtcttccag actgtcaaga gcctgcggct acagaggcca
     3121 cacatggtcc agacactgga acagtatgag ttctgctaca aggtggtgca ggagtatatt
     3181 gatgcattct cagattatgc caacttcaag ttggacccag ctttcttgta caaagtggtg
     3241 agctccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3301 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3361 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3421 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3481 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3541 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3601 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3661 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3721 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     3781 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3841 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     3901 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3961 ctgtacaagc accggtccac gtgacgtaag ctagatccag cacagtggcg gccgctcgag
     4021 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4081 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4141 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4201 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4261 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4321 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4381 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4561 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     4621 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     4681 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     4741 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     4801 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     4861 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     4921 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     4981 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5041 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5101 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5161 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5221 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5281 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5341 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5401 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5461 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5521 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5581 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     5641 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5701 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5761 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5821 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5881 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     5941 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6781 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7021 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8041 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8101 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     8161 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8221 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8281 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
