LOCUS       sequence-275769-       10355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a VEGFR2-mCherry fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Pietila I, Van Mourik D, Tamelander A, Kriz V, Claesson-Welsh L, 
            Tengholm A, Welsh M
  TITLE     Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend
            on SHB.
  JOURNAL   Cells. 2019 Dec 15;8(12). pii: cells8121645. doi: 
            10.3390/cells8121645.
  PUBMED    31847469
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-12-15"; volume: "8(12). pii: cells8121645. doi"; pages: 
            " 10.3390/cells8121645"
COMMENT     Sequence Label: pcDNA3.1-VEGFR2-mCherry
FEATURES             Location/Qualifiers
     source          1..10355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5021..5731
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5166..5184)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5434..5453
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5777..5794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5783..6007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6053..6481
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6140..6159)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6350..6371
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6490..6510)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6495..6824
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6675..6810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6737..6756
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6873..7898
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6873..7898
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8028..8149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8065..8084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8119..8138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8198..8214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8198..8214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8211..8233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8222..8238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8246..8252,8253..8270,8271..8276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8291..8312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8429..8446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8600..9188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8680..8699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9359..10150,10151..10219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9982..10001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10220..10324)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatgcagag caaggtgctg ctggccgtcg ccctgtggct
      961 ctgcgtggag acccgggccg cctctgtggg tttgcctagt gtttctcttg atctgcccag
     1021 gctcagcata caaaaagaca tacttacaat taaggctaat acaactcttc aaattacttg
     1081 caggggacag agggacttgg actggctttg gcccaataat cagagtggca gtgagcaaag
     1141 ggtggaggtg actgagtgca gcgatggcct cttctgtaag acactcacaa ttccaaaagt
     1201 gatcggaaat gacactggag cctacaagtg cttctaccgg gaaactgact tggcctcggt
     1261 catttatgtc tatgttcaag attacagatc tccatttatt gcttctgtta gtgaccaaca
     1321 tggagtcgtg tacattactg agaacaaaaa caaaactgtg gtgattccat gtctcgggtc
     1381 catttcaaat ctcaacgtgt cactttgtgc aagataccca gaaaagagat ttgttcctga
     1441 tggtaacaga atttcctggg acagcaagaa gggctttact attcccagct acatgatcag
     1501 ctatgctggc atggtcttct gtgaagcaaa aattaatgat gaaagttacc agtctattat
     1561 gtacatagtt gtcgttgtag ggtataggat ttatgatgtg gttctgagtc cgtctcatgg
     1621 aattgaacta tctgttggag aaaagcttgt cttaaattgt acagcaagaa ctgaactaaa
     1681 tgtggggatt gacttcaact gggaataccc ttcttcgaag catcagcata agaaacttgt
     1741 aaaccgagac ctaaaaaccc agtctgggag tgagatgaag aaatttttga gcaccttaac
     1801 tatagatggt gtaacccgga gtgaccaagg attgtacacc tgtgcagcat ccagtgggct
     1861 gatgaccaag aagaacagca catttgtcag ggtccatgaa aaaccttttg ttgcttttgg
     1921 aagtggcatg gaatctctgg tggaagccac ggtgggggag cgtgtcagaa tccctgcgaa
     1981 gtaccttggt tacccacccc cagaaataaa atggtataaa aatggaatac cccttgagtc
     2041 caatcacaca attaaagcgg ggcatgtact gacgattatg gaagtgagtg aaagagacac
     2101 aggaaattac actgtcatcc ttaccaatcc catttcaaag gagaagcaga gccatgtggt
     2161 ctctctggtt gtgtatgtcc caccccagat tggtgagaaa tctctaatct ctcctgtgga
     2221 ttcctaccag tacggcacca ctcaaacgct gacatgtacg gtctatgcca ttcctccccc
     2281 gcatcacatc cactggtatt ggcagttgga ggaagagtgc gccaacgagc ccagccaagc
     2341 tgtctcagtg acaaacccat acccttgtga agaatggaga agtgtggagg acttccaggg
     2401 aggaaataaa attgaagtta ataaaaatca atttgctcta attgaaggaa aaaacaaaac
     2461 tgtaagtacc cttgttatcc aagcggcaaa tgtgtcagct ttgtacaaat gtgaagcggt
     2521 caacaaagtc gggagaggag agagggtgat ctccttccac gtgaccaggg gtcctgaaat
     2581 tactttgcaa cctgacatgc agcccactga gcaggagagc gtgtctttgt ggtgcactgc
     2641 agacagatct acgtttgaga acctcacatg gtacaagctt ggcccacagc ctctgccaat
     2701 ccatgtggga gagttgccca cacctgtttg caagaacttg gatactcttt ggaaattgaa
     2761 tgccaccatg ttctctaata gcacaaatga cattttgatc atggagctta agaatgcatc
     2821 cttgcaggac caaggagact atgtctgcct tgctcaagac aggaagacca agaaaagaca
     2881 ttgcgtggtc aggcagctca cagtcctaga gcgtgtggca cccacgatca caggaaacct
     2941 ggagaatcag acgacaagta ttggggaaag catcgaagtc tcatgcacgg catctgggaa
     3001 tccccctcca cagatcatgt ggtttaaaga taatgagacc cttgtagaag actcaggcat
     3061 tgtattgaag gatgggaacc ggaacctcac tatccgcaga gtgaggaagg aggacgaagg
     3121 cctctacacc tgccaggcat gcagtgttct tggctgtgca aaagtggagg catttttcat
     3181 aatagaaggt gcccaggaaa agacgaactt ggaaatcatt attctagtag gcacggcggt
     3241 gattgccatg ttcttctggc tacttcttgt catcatccta cggaccgtta agcgggccaa
     3301 tggaggggaa ctgaagacag gctacttgtc catcgtcatg gacccagatg aactcccatt
     3361 ggatgaacat tgtgaacgac tgccttatga tgccagcaaa tgggaattcc ccagagaccg
     3421 gctgaagcta ggtaagcctc ttggccgtgg tgcctttggc caagtgattg aagcagatgc
     3481 ctttggaatt gacaagacag caacttgcag gacagtagca gtcaaaatgt tgaaagaagg
     3541 agcaacacac agtgagcatc gagctctcat gtctgaactc aagatcctca ttcatattgg
     3601 tcaccatctc aatgtggtca accttctagg tgcctgtacc aagccaggag ggccactcat
     3661 ggtgattgtg gaattctgca aatttggaaa cctgtccact tacctgagga gcaagagaaa
     3721 tgaatttgtc ccctacaaga ccaaaggggc acgattccgt caagggaaag actacgttgg
     3781 agcaatccct gtggatctga aacggcgctt ggacagcatc accagtagcc agagctcagc
     3841 cagctctgga tttgtggagg agaagtccct cagtgatgta gaagaagagg aagctcctga
     3901 agatctgtat aaggacttcc tgaccttgga gcatctcatc tgttacagct tccaagtggc
     3961 taagggcatg gagttcttgg catcgcgaaa gtgtatccac agggacctgg cggcacgaaa
     4021 tatcctctta tcggagaaga acgtggttaa aatctgtgac tttggcttgg cccgggatat
     4081 ttataaagat ccagattatg tcagaaaagg agatgctcgc ctccctttga aatggatggc
     4141 cccagaaaca atttttgaca gagtgtacac aatccagagt gacgtctggt cttttggtgt
     4201 tttgctgtgg gaaatatttt ccttaggtgc ttctccatat cctggggtaa agattgatga
     4261 agaattttgt aggcgattga aagaaggaac tagaatgagg gcccctgatt atactacacc
     4321 agaaatgtac cagaccatgc tggactgctg gcacggggag cccagtcaga gacccacgtt
     4381 ttcagagttg gtggaacatt tgggaaatct cttgcaagct aatgctcagc aggatggcaa
     4441 agactacatt gttcttccga tatcagagac tttgagcatg gaagaggatt ctggactctc
     4501 tctgcctacc tcacctgttt cctgtatgga ggaggaggaa gtatgtgacc ccaaattcca
     4561 ttatgacaac acagcaggaa tcagtcagta tctgcagaac agtaagcgaa agagccggcc
     4621 tgtgagtgta aaaacatttg aagatatccc gttagaagaa ccagaagtaa aagtaatccc
     4681 agatgacaac cagacggaca gtggtatggt tcttgcctca gaagagctga aaactttgga
     4741 agacagaacc aaattatctc catcttttgg tggaatggtg cccagcaaaa gcagggagtc
     4801 tgtggcatct gaaggctcaa accagacaag cggctaccag tccggatatc actccgatga
     4861 cacagacacc accgtgtact ccagtgagga agcagaactt ttaaagctga tagagattgg
     4921 agtgcaaacc ggtagcacag cccagattct ccagcctgac tcggggacca cactgagctc
     4981 tcctcctgtt ggatccggtg ggagtggagg tgggagtgga atggtgagca agggcgagga
     5041 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     5101 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5161 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     5221 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     5281 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5341 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     5401 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     5461 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     5521 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     5581 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     5641 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     5701 caccggcggc atggacgagc tgtacaagta aagcggccgc tcgagtctag agggcccgtt
     5761 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5821 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5881 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5941 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6001 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6061 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6121 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6181 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6241 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6301 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6361 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6421 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6481 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6541 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6601 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6661 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6781 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6841 gtatatccat tttcggatct gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt
     6901 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     6961 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     7021 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     7081 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     7141 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     7201 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     7261 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     7321 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     7381 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     7441 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     7501 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     7561 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     7621 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     7681 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     7741 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     7801 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     7861 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtgctacga gatttcgatt
     7921 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7981 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     8041 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8101 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     8161 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8221 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8281 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8341 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8401 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8461 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8521 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9001 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9121 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9241 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9301 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9361 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9421 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9481 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9541 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9661 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9961 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10321 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
