LOCUS       sequence-275770-       10447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Pietila I, Van Mourik D, Tamelander A, Kriz V, Claesson-Welsh L, 
            Tengholm A, Welsh M
  TITLE     Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend
            on SHB.
  JOURNAL   Cells. 2019 Dec 15;8(12). pii: cells8121645. doi: 
            10.3390/cells8121645.
  PUBMED    31847469
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-12-15"; volume: "8(12). pii: cells8121645. doi"; pages: 
            " 10.3390/cells8121645"
COMMENT     Sequence Label: pcDNA3.1-Y1175F-VEGFR2-mCherry
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5113..5823
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5258..5276)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5526..5545
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5869..5886)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5875..6099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6145..6573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6232..6251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6442..6463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6582..6602)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6587..6916
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6767..6902
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6829..6848
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6965..7990
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6965..7990
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8120..8241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8157..8176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8211..8230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8290..8306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8290..8306)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8303..8325)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8314..8330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8338..8344,8345..8362,8363..8368))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8383..8404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8521..8538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8692..9280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8772..8791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9451..10242,10243..10311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10074..10093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10312..10416)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agattcgagg tcgacgcgcc atggagctcc atggcgcgtc gacgcgccat ggagaattcc
     1021 agcaaggtgc tgctggccgt cgccctgtgg ctctgcgtgg agacccgggc cgcctctgtg
     1081 ggtttgccta gtgtttctct tgatctgccc aggctcagca tacaaaaaga catacttaca
     1141 attaaggcta atacaactct tcaaattact tgcaggggac agagggactt ggactggctt
     1201 tggcccaata atcagagtgg cagtgagcaa agggtggagg tgactgagtg cagcgatggc
     1261 ctcttctgta agacactcac aattccaaaa gtgatcggaa atgacactgg agcctacaag
     1321 tgcttctacc gggaaactga cttggcctcg gtcatttatg tctatgttca agattacaga
     1381 tctccattta ttgcttctgt tagtgaccaa catggagtcg tgtacattac tgagaacaaa
     1441 aacaaaactg tggtgattcc atgtctcggg tccatttcaa atctcaacgt gtcactttgt
     1501 gcaagatacc cagaaaagag atttgttcct gatggtaaca gaatttcctg ggacagcaag
     1561 aagggcttta ctattcccag ctacatgatc agctatgctg gcatggtctt ctgtgaagca
     1621 aaaattaatg atgaaagtta ccagtctatt atgtacatag ttgtcgttgt agggtatagg
     1681 atttatgatg tggttctgag tccgtctcat ggaattgaac tatctgttgg agaaaagctt
     1741 gtcttaaatt gtacagcaag aactgaacta aatgtgggga ttgacttcaa ctgggaatac
     1801 ccttcttcga agcatcagca taagaaactt gtaaaccgag acctaaaaac ccagtctggg
     1861 agtgagatga agaaattttt gagcacctta actatagatg gtgtaacccg gagtgaccaa
     1921 ggattgtaca cctgtgcagc atccagtggg ctgatgacca agaagaacag cacatttgtc
     1981 agggtccatg aaaaaccttt tgttgctttt ggaagtggca tggaatctct ggtggaagcc
     2041 acggtggggg agcgtgtcag aatccctgcg aagtaccttg gttacccacc cccagaaata
     2101 aaatggtata aaaatggaat accccttgag tccaatcaca caattaaagc ggggcatgta
     2161 ctgacgatta tggaagtgag tgaaagagac acaggaaatt acactgtcat ccttaccaat
     2221 cccatttcaa aggagaagca gagccatgtg gtctctctgg ttgtgtatgt cccaccccag
     2281 attggtgaga aatctctaat ctctcctgtg gattcctacc agtacggcac cactcaaacg
     2341 ctgacatgta cggtctatgc cattcctccc ccgcatcaca tccactggta ttggcagttg
     2401 gaggaagagt gcgccaacga gcccagccaa gctgtctcag tgacaaaccc atacccttgt
     2461 gaagaatgga gaagtgtgga ggacttccag ggaggaaata aaattgaagt taataaaaat
     2521 caatttgctc taattgaagg aaaaaacaaa actgtaagta cccttgttat ccaagcggca
     2581 aatgtgtcag ctttgtacaa atgtgaagcg gtcaacaaag tcgggagagg agagagggtg
     2641 atctccttcc acgtgaccag gggtcctgaa attactttgc aacctgacat gcagcccact
     2701 gagcaggaga gcgtgtcttt gtggtgcact gcagacagat ctacgtttga gaacctcaca
     2761 tggtacaagc ttggcccaca gcctctgcca atccatgtgg gagagttgcc cacacctgtt
     2821 tgcaagaact tggatactct ttggaaattg aatgccacca tgttctctaa tagcacaaat
     2881 gacattttga tcatggagct taagaatgca tccttgcagg accaaggaga ctatgtctgc
     2941 cttgctcaag acaggaagac caagaaaaga cattgcgtgg tcaggcagct cacagtccta
     3001 gagcgtgtgg cacccacgat cacaggaaac ctggagaatc agacgacaag tattggggaa
     3061 agcatcgaag tctcatgcac ggcatctggg aatccccctc cacagatcat gtggtttaaa
     3121 gataatgaga cccttgtaga agactcaggc attgtattga aggatgggaa ccggaacctc
     3181 actatccgca gagtgaggaa ggaggacgaa ggcctctaca cctgccaggc atgcagtgtt
     3241 cttggctgtg caaaagtgga ggcatttttc ataatagaag gtgcccagga aaagacgaac
     3301 ttggaaatca ttattctagt aggcacggcg gtgattgcca tgttcttctg gctacttctt
     3361 gtcatcatcc tacggaccgt taagcgggcc aatggagggg aactgaagac aggctacttg
     3421 tccatcgtca tggatccaga tgaactccca ttggatgaac attgtgaacg actgccttat
     3481 gatgccagca aatgggaatt ccccagagac cggctgaagc taggtaagcc tcttggccgt
     3541 ggtgcctttg gccaagtgat tgaagcagat gcctttggaa ttgacaagac agcaacttgc
     3601 aggacagtag cagtcaaaat gttgaaagaa ggagcaacac acagtgagca tcgagctctc
     3661 atgtctgaac tcaagatcct cattcatatt ggtcaccatc tcaatgtggt caaccttcta
     3721 ggtgcctgta ccaagccagg agggccactc atggtgattg tggaattctg caaatttgga
     3781 aacctgtcca cttacctgag gagcaagaga aatgaatttg tcccctacaa gaccaaaggg
     3841 gcacgattcc gtcaagggaa agactacgtt ggagcaatcc ctgtggatct gaaacggcgc
     3901 ttggacagca tcaccagtag ccagagctca gccagctctg gatttgtgga ggagaagtcc
     3961 ctcagtgatg tagaagaaga ggaagctcct gaagatctgt ataaggactt cctgaccttg
     4021 gagcatctca tctgttacag cttccaagtg gctaagggca tggagttctt ggcatcgcga
     4081 aagtgtatcc acagggacct ggcggcacga aatatcctct tatcggagaa gaacgtggtt
     4141 aaaatctgtg actttggctt ggcccgggat atttataaag atccagatta tgtcagaaaa
     4201 ggagatgctc gcctcccttt gaaatggatg gccccagaaa caatttttga cagagtgtac
     4261 acaatccaga gtgacgtctg gtcttttggt gttttgctgt gggaaatatt ttccttaggt
     4321 gcttctccat atcctggggt aaagattgat gaagaatttt gtaggcgatt gaaagaagga
     4381 actagaatga gggcccctga ttatactaca ccagaaatgt accagaccat gctggactgc
     4441 tggcacgggg agcccagtca gagacccacg ttttcagagt tggtggaaca tttgggaaat
     4501 ctcttgcaag ctaatgctca gcaggatggc aaagacttca ttgttcttcc gatatcagag
     4561 actttgagca tggaagagga ttctggactc tctctgccta cctcacctgt ttcctgtatg
     4621 gaggaggagg aagtatgtga ccccaaattc cattatgaca acacagcagg aatcagtcag
     4681 tatctgcaga acagtaagcg aaagagccgg cctgtgagtg taaaaacatt tgaagatatc
     4741 ccgttagaag aaccagaagt aaaagtaatc ccagatgaca accagacgga cagtggtatg
     4801 gttcttgcct cagaagagct gaaaactttg gaagacagaa ccaaattatc tccatctttt
     4861 ggtggaatgg tgcccagcaa aagcagggag tctgtggcat ctgaaggctc aaaccagaca
     4921 agcggctacc agtccggata tcactccgat gacacagaca ccaccgtgta ctccagtgag
     4981 gaagcagaac ttttaaagct gatagagatt ggagtgcaaa ccggtagcac agcccagatt
     5041 ctccagcctg actcggggac cacactgagc tctcctcctg ttggatccgg tgggagtgga
     5101 ggtgggagtg gaatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5161 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5221 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5281 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5341 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5401 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5461 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5521 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5581 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5641 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5701 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5761 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5821 taaagcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5881 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5941 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6001 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6061 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6121 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6181 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6241 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6301 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6361 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6421 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6481 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6541 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6721 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6841 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6901 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6961 cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     7021 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     7081 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     7141 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     7201 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     7261 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     7321 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     7381 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     7441 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     7501 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     7561 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     7621 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     7681 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     7741 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     7801 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     7861 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     7921 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     7981 aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     8041 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     8101 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     8161 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8221 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     8281 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8341 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8401 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8461 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10441 tgacgtc
//
