LOCUS       sequence-275774-        8580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  StrepIII-HA-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: RET_Y981F-SHC
FEATURES             Location/Qualifiers
     source          1..8580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4043..4067)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4114..4137
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4174..4197
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4228..4254
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4228..4254
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4298..4315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4304..4528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4661..4680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4812..4859
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4867..5889
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4867..5889
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6019..6140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6056..6075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6110..6129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6189..6205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6189..6205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6202..6224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6213..6229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6237..6243,6244..6261,6262..6267))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6282..6303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6420..6437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6591..7179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6671..6690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7350..8141,8142..8210))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7973..7992
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8211..8315)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8390..8409)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcaccat ggcgaaggcg
      841 acgtccggtg ccgcggggct gcgtctgctg ttgctgctgc tgctgccgct gctaggcaaa
      901 gtggcattgg gcctctactt ctcgagggat gcttactggg agaagctgta tgtggaccag
      961 gcggccggca cgcccttgct gtacgtccat gccctgcggg acgcccctga ggaggtgccc
     1021 agcttccgcc tgggccagca tctctacggc acgtaccgca cacggctgca tgagaacaac
     1081 tggatctgca tccaggagga caccggcctc ctctacctta accggagcct ggaccatagc
     1141 tcctgggaga agctcagtgt ccgcaaccgc ggctttcccc tgctcaccgt ctacctcaag
     1201 gtcttcctgt cacccacatc ccttcgtgag ggcgagtgcc agtggccagg ctgtgcccgc
     1261 gtatacttct ccttcttcaa cacctccttt ccagcctgca gctccctcaa gccccgggag
     1321 ctctgcttcc cagagacaag gccctccttc cgcattcggg agaaccgacc cccaggcacc
     1381 ttccaccagt tccgcctgct gcctgtgcag ttcttgtgcc ccaacatcag cgtggcctac
     1441 aggctcctgg agggtgaggg tctgcccttc cgctgcgccc cggacagcct ggaggtgagc
     1501 acgcgctggg ccctggaccg cgagcagcgg gagaagtacg agctggtggc cgtgtgcacc
     1561 gtgcacgccg gcgcgcgcga ggaggtggtg atggtgccct tcccggtgac cgtgtacgac
     1621 gaggacgact cggcgcccac cttccccgcg ggcgtcgaca ccgccagcgc cgtggtggag
     1681 ttcaagcgga aggaggacac cgtggtggcc acgctgcgtg tcttcgatgc agacgtggta
     1741 cctgcatcag gggagctggt gaggcggtac acaagcacgc tgctccccgg ggacacctgg
     1801 gcccagcaga ccttccgggt ggaacactgg cccaacgaga cctcggtcca ggccaacggc
     1861 agcttcgtgc gggcgaccgt acatgactat aggctggttc tcaaccggaa cctctccatc
     1921 tcggagaacc gcaccatgca gctggcggtg ctggtcaatg actcagactt ccagggccca
     1981 ggagcgggcg tcctcttgct ccacttcaac gtgtcggtgc tgccggtcag cctgcacctg
     2041 cccagtacct actccctctc cgtgagcagg agggctcgcc gatttgccca gatcgggaaa
     2101 gtctgtgtgg aaaactgcca ggcattcagt ggcatcaacg tccagtacaa gctgcattcc
     2161 tctggtgcca actgcagcac gctaggggtg gtcacctcag ccgaggacac ctcggggatc
     2221 ctgtttgtga atgacaccaa ggccctgcgg cggcccaagt gtgccgaact tcactacatg
     2281 gtggtggcca ccgaccagca gacctctagg caggcccagg cccagctgct tgtaacagtg
     2341 gaggggtcat atgtggccga ggaggcgggc tgccccctgt cctgtgcagt cagcaagaga
     2401 cggctggagt gtgaggagtg tggcggcctg ggctccccaa caggcaggtg tgagtggagg
     2461 caaggagatg gcaaagggat caccaggaac ttctccacct gctctcccag caccaagacc
     2521 tgccccgacg gccactgcga tgttgtggag acccaagaca tcaacatttg ccctcaggac
     2581 tgcctccggg gcagcattgt tgggggacac gagcctgggg agccccgggg gattaaagct
     2641 ggctatggca cctgcaactg cttccctgag gaggagaagt gcttctgcga gcccgaagac
     2701 atccaggatc cactgtgcga cgagctgtgc cgcacggtga tcgcagccgc tgtcctcttc
     2761 tccttcatcg tctcggtgct gctgtctgcc ttctgcatcc actgctacca caagtttgcc
     2821 cacaagccac ccatctcctc agctgagatg accttccgga ggcccgccca ggccttcccg
     2881 gtcagctact cctcttccag tgcccgccgg ccctcgctgg actccatgga gaaccaggtc
     2941 tccgtggatg ccttcaagat cctggaggat ccaaagtggg aattccctcg gaagaacttg
     3001 gttcttggaa aaactctagg agaaggcgaa tttggaaaag tggtcaaggc aacggccttc
     3061 catctgaaag gcagagcagg gtacaccacg gtggccgtga agatgctgaa agagaacgcc
     3121 tccccgagtg agcttcgaga cctgctgtca gagttcaacg tcctgaagca ggtcaaccac
     3181 ccacatgtca tcaaattgta tggggcctgc agccaggatg gcccgctcct cctcatcgtg
     3241 gagtacgcca aatacggctc cctgcggggc ttcctccgcg agagccgcaa agtggggcct
     3301 ggctacctgg gcagtggagg cagccgcaac tccagctccc tggaccaccc ggatgagcgg
     3361 gccctcacca tgggcgacct catctcattt gcctggcaga tctcacaggg gatgcagtat
     3421 ctggccgaga tgaagctcgt tcatcgggac ttggcagcca gaaacatcct ggtagctgag
     3481 gggcggaaga tgaagatttc ggatttcggc ttgtcccgag atgtttatga agaggattcg
     3541 tacgtgaaga ggagccaggg tcggattcca gttaaatgga tggcaattga atcccttttt
     3601 gatcatatct acaccacgca aagtgatgta tggtcttttg gtgtcctgct gtgggagatc
     3661 gtgaccctag ggggaaaccc ctatcctggg attcctcctg agcggctctt caaccttctg
     3721 aagaccggcc accggatgga gaggccagac aactgcagcg aggagatgtt ctgcctgatg
     3781 ctgcaatgct ggaagcagga gccggacaaa aggccggtgt ttgcggacat cagcaaagac
     3841 ctggagaaga tgatggttaa gaggagagac tacttggacc ttgcggcgtc cactccatct
     3901 gactccctga tttatgacga cggcctctca gaggaggaga caccgctggt ggactgtaat
     3961 aatgcccccc tccctcgagc cctcccttcc acatggattg aaaacaaact ctatggtaga
     4021 atttcccatg catttactag attcccaact ttcttgtaca aagtggttga tatcaaggcc
     4081 gccgacatca ccagcctgta caagaaagtg gggtggagcc atccccagtt cgagaagggc
     4141 ggaggatctg gcggcggaag cggcggagga agctggtccc accctcagtt tgagaaaggc
     4201 accgagctgg gcagcaccat ggccagctac ccctacgacg tgcccgacta cgcctgatga
     4261 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4321 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4381 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4441 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4501 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4561 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4621 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4681 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4741 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4801 tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
     4861 ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     4921 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     4981 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     5041 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     5101 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     5161 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     5221 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     5281 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     5341 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5401 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5461 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     5521 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     5581 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     5641 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     5701 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     5761 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     5821 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     5881 aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5941 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6001 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6121 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6181 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6241 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6301 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6361 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6421 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6481 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6541 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6601 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6661 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6721 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6781 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6841 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6901 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6961 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7021 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7081 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7141 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7201 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7261 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7321 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7381 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7441 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7501 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7561 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7621 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7681 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7741 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7801 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7861 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7921 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7981 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8041 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8101 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8161 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8221 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8281 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8341 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     8401 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8461 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8521 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
