LOCUS       sequence-275777-        7757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Clostridial vector, encoding the Cre protein, which mediates 
            recombination between lox sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC
  TITLE     A Targetron-Recombinase System for Large-Scale Genome Engineering of
            Clostridia.
  JOURNAL   mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 
            10.1128/mSphere.00710-19.
  PUBMED    31826971
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-12-11"; volume: "4(6). pii: 4/6/e00710-19. doi"; pages: 
            " 10.1128/mSphere.00710-19"
COMMENT     Sequence Label: pQcre1
FEATURES             Location/Qualifiers
     source          1..7757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      386..974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     875..894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1128..1145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1262..1283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1298..1303,1304..1321,1322..1328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1336..1352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1341..1363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1360..1376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1360..1376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1422..2453)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     complement(2639..2656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2639..2655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2648..2670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2981..3352)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3385..3494
                     /note="incP origin of transfer"
ORIGIN
        1 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
       61 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
      121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
      181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
      241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
      301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     1261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     1321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     1381 attacgccaa gcttgcatgc ctgcaggtcg actctagatt actaatcgcc atcttccagc
     1441 aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt
     1501 acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata
     1561 tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag
     1621 tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga
     1681 gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac
     1741 aggtagttat tcggatcatc agctacacca gagacggaaa tccatcgctc gaccagttta
     1801 gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg
     1861 ccaatatgga ttaacatcct cccaccgtca gtacgtgaga tatctttaac cctgatcctg
     1921 gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt
     1981 atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt
     2041 tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc
     2101 cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga
     2161 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga
     2221 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc
     2281 aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca ggtatgctca
     2341 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt
     2401 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga catatgcgtt
     2461 ctcctttttg tataaaatta aatttatatt atattgaaat taataaactt tagtttcaat
     2521 ataatataaa taaaaagaga taattattta tttttggctt tttaaacaga acttttataa
     2581 atatataaca taaaaaagaa tttatatgtt taataggatg aatccgaacc tcgaattcac
     2641 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2701 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2761 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     2821 cgcatctgtg cggtatttca caccgcatag gggatcggtc ttgccttgct cgtcggtgat
     2881 gtacttcacc agctccgcga agtcgctctt cttgatggag cgcatgggga cgtgcttggc
     2941 aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct gccctcgggc
     3001 ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca
     3061 gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt
     3121 tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct
     3181 catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca
     3241 ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt
     3301 acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc
     3361 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca
     3421 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt
     3481 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt
     3541 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga
     3601 ccccgaagca gggttatgca gcggaaaaga tcccccgaag caaacttaag agtgtgttga
     3661 tagtgcagta tcttaaaatt ttgtgtataa taggaattga agttaaatta gatgctaaaa
     3721 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgta atgtagataa
     3781 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
     3841 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
     3901 caaatcgttt aacttctgta tttatttaca gatgtaatca cttcaggagt aattacatga
     3961 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
     4021 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
     4081 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
     4141 atctattcaa cttatcgtca gaaaaattaa aactgaacat tcgtgtcact ttaattcacc
     4201 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc
     4261 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca
     4321 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
     4381 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
     4441 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
     4501 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
     4561 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     4621 atttaagtac cattacttat gagcaagtat tgtctatttt taatagttat ctattattta
     4681 acgggaggaa ataattctat gagtcgcttt tttaaatttg gaaagttaca cgttactaaa
     4741 gggaatggag ataaattatt agatatacta ctgacagctt ccaagaagct aaagaggtcc
     4801 ctagcgccta cggggaattt gggcaaatca aaagaaaaat tctcccaaga attagaaatg
     4861 agtagatcaa attattcacg aatagaatca ggaaaatcag atccaaccat aaaaacacta
     4921 gaacaaattg caaagttaac taactcaacg ctagtagtgg atttaatccc aaatgagcca
     4981 acagaaccag aaccagaaac agaatcagaa caagtaacat tggatttaga aatggaagaa
     5041 gaaaaaagca atgacttcgt gtgaataatg cacgaaatcg ttgcttattt tttttaaaag
     5101 cggtatacta gatataacga aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac
     5161 atttataaaa tgtttgacga cattttataa atgcatagcc cgataagatt gccaaaccaa
     5221 cgcttatcag ttagtcagat gaactcttcc ctcgtaagaa gttatttaat taactttgtt
     5281 tgaagacggt atataaccgt actatcatta tatagggaaa tcagagagtt ttcaagtatc
     5341 taagctactg aatttaagaa ttgttaagca atcaatcgga aatcgtttga ttgctttttt
     5401 tgtattcatt tatagaaggt ggagtttgta tgaatcatga tgaatgtaaa acttatataa
     5461 aaaatagttt attggagata agaaaattag caaatatcta tacactagaa acgtttaaga
     5521 aagagttaga aaagagaaat atctacttag aaacaaaatc agataagtat ttttcttcgg
     5581 agggggaaga ttatatatat aagttaatag aaaataacaa aataatttat tcgattagtg
     5641 gaaaaaaatt gacttataaa ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt
     5701 tgaatgaaaa agcaaaccaa gttaattaaa caacctattt tataggattt ataggaaagg
     5761 agaacagctg aatgaatatc ccttttgttg tagaaactgt gcttcatgac ggcttgttaa
     5821 agtacaaatt taaaaatagt aaaattcgct caatcactac caagccaggt aaaagcaaag
     5881 gggctatttt tgcgtatcgc tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga
     5941 cttccgagga agcgattcaa gaaaatcaag atacatttac acattggaca cccaacgttt
     6001 atcgttatgg aacgtatgca gacgaaaacc gttcatacac gaaaggacat tctgaaaaca
     6061 atttaagaca aatcaatacc ttctttattg attttgatat tcacacggca aaagaaacta
     6121 tttcagcaag cgatatttta acaaccgcta ttgatttagg ttttatgcct actatgatta
     6181 tcaaatctga taaaggttat caagcatatt ttgttttaga aacgccagtc tatgtgactt
     6241 caaaatcaga atttaaatct gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat
     6301 attttggaaa gtctttgcca gttgatctaa cgtgtaatca ttttggtatt gctcgcatac
     6361 caagaacgga caatgtagaa ttttttgatc ctaattaccg ttattctttc aaagaatggc
     6421 aagattggtc tttcaaacaa acagataata agggctttac tcgttcaagt ctaacggttt
     6481 taagcggtac agaaggcaaa aaacaagtag atgaaccctg gtttaatctc ttattgcacg
     6541 aaacgaaatt ttcaggagaa aagggtttaa tagggcgtaa taacgtcatg tttaccctct
     6601 ctttagccta ctttagttca ggctattcaa tcgaaacgtg cgaatataat atgtttgagt
     6661 ttaataatcg attagatcaa cccttagaag aaaaagaagt aatcaaaatt gttagaagtg
     6721 cctattcaga aaactatcaa ggggctaata gggaatacat taccattctt tgcaaagctt
     6781 gggtatcaag tgatttaacc agtaaagatt tatttgtccg tcaagggtgg tttaaattca
     6841 agaaaaaaag aagcgaacgt caacgtgttc atttgtcaga atggaaagaa gatttaatgg
     6901 cttatattag cgaaaaaagc gatgtataca agccttattt agtgacgacc aaaaaagaga
     6961 ttagagaagt gctaggcatt cctgaacgga cattagataa attgctgaag gtactgaagg
     7021 cgaatcagga aattttcttt aagattaaac caggaagaaa tggtggcatt caacttgcta
     7081 gtgttaaatc attgttgcta tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata
     7141 taaaggcgct gacaaattct tttgacttag agcatacatt cattcaagag actttaaaca
     7201 agctagcaga acgccctaaa acggacacac aactcgattt gtttagctat gatacaggct
     7261 gaaaataaaa cccgcactat gccattacat ttatatctat gatacgtgtt tgttttttct
     7321 ttgctgttta gcgaatgatt agcagaaata tacagagtaa gattttaatt aattattagg
     7381 gggagaagga gagagtagcc cgaaaacttt tagttggctt ggactgaacg aagtgaggga
     7441 aaggctacta aaacgtcgag gggcagtgag agcgaagcga acacttgatt ttttaatttt
     7501 ctatctttta taggtcatta gagtatactt atttgtccta taaactattt agcagcataa
     7561 tagatttatt gaataggtca tttaagttga gcatattaga ggaggaaaat cttggagaaa
     7621 tatttgaaga acccgattac atggattgga ttagttcttg tggttacgtg gtttttaact
     7681 aaaagtagtg aatttttgat ttttggtgtg tgtgtcttgt tgttagtatt tgctagtcaa
     7741 agtgattaaa tagaatt
//
