LOCUS       sequence-275781-        5857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses luciferase under the promoter of CAG. Transgene flanked by
            AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Wang S, Li Y, Xu Y, Ma Q, Lin Z, Schlame M, Bezzerides VJ, Strathdee
            D, Pu WT
  TITLE     AAV Gene Therapy Prevents and Reverses Heart Failure in A Murine 
            Knockout Model of Barth Syndrome.
  JOURNAL   Circ Res. 2020 Mar 9. doi: 10.1161/CIRCRESAHA.119.315956.
  PUBMED    32146862
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Mar 9. doi: 10.1161/CIRCRESAHA.119.315956."
COMMENT     Sequence Label: pCAG/Luci
FEATURES             Location/Qualifiers
     source          1..5857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        148..425
                     /label=chicken beta-actin promoter
     primer_bind     704..726
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     788..807
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             855..2507
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(921..939)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2531..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2542..2561)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2596..2615
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3180..3768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3260..3279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3939..4730,4731..4799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4562..4581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4800..4904)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4930..5385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5067..5088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5279..5298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5565..5694
                     /label=AAV2 ITR
ORIGIN
        1 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
       61 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      121 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
      181 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
      241 cagcgatggg ggcggggggg gggggggggc gcgcgccagg sggggcgggg cggggcgagg
      301 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      361 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      421 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
      481 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
      541 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
      601 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggctgt
      661 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
      721 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
      781 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attggatccc
      841 cgggtaccgc tagcatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
      901 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
      961 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     1021 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     1081 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     1141 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     1201 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     1261 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     1321 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     1381 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     1441 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     1501 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     1561 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     1621 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     1681 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     1741 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     1801 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     1861 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     1921 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     1981 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     2041 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     2101 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     2161 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     2221 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     2281 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     2341 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     2401 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     2461 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaagcg gccgcttcga
     2521 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2581 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2641 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     2701 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt
     2761 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     2821 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgyscg
     2881 skckktssyc sgrcgscgsc sgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     2941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4921 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4981 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5041 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5101 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5161 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5221 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5281 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5341 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     5401 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc
     5461 gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcksgsg mccyytkgtc
     5521 gcccggcctc agtgassgwg cgrgcctcyw cgctattacg ccagctgcgc gctcgctcgc
     5581 tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag
     5641 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt
     5701 taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag agtaccattg
     5761 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5821 tgggtggagt atttacggta aactgcccac ttggcag
//
