LOCUS       sequence-275815-        8350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible polycistronic lentiviral vector expressing mouse Oct4,
            Sox2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002."
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-OS
FEATURES             Location/Qualifiers
     source          1..8350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    4792..5380
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4845..4865)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5263..5274)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5528..5708
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5755..5773)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5841..5945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5946..6014,6015..6806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6164..6183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6977..7565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7719..7736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7976..7997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8012..8017,8018..8035,8036..8042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8050..8066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8055..8077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8074..8090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8074..8090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8140..8159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac gctagcggca gcggcgccac gaacttctct ctgttaaagc
     3781 aagcaggaga tgttgaagaa aaccccgggc ctgcatgcat gtataacatg atggagacgg
     3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca
     3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga
     3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga
     4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg
     4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgc tctgcacatg aaggagcacc
     4141 cggattataa ataccggccg cggcggaaaa ccaagacgct catgaagaag gataagtaca
     4201 cgcttcccgg aggcttgctg gcccccggcg ggaacagcat ggcgagcggg gttggggtgg
     4261 gcgccggcct gggtgcgggc gtgaaccagc gcatggacag ctacgcgcac atgaacggct
     4321 ggagcaacgg cagctacagc atgatgcagg agcagctggg ctacccgcag cacccgggcc
     4381 tcaacgctca cggcgcggca cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc
     4441 agtacaactc catgaccagc tcgcagacct acatgaacgg ctcgcccacc tacagcatgt
     4501 cctactcgca gcagggcacc cccggtatgg cgctgggctc catgggctct gtggtcaagt
     4561 ccgaggccag ctccagcccc cccgtggtta cctcttcctc ccactccagg gcgccctgcc
     4621 aggccgggga cctccgggac atgatcagca tgtacctccc cggcgccgag gtgccggagc
     4681 ccgctgcgcc cagtagactg cacatggccc agcactacca gagcggcccg gtgcccggca
     4741 cggccattaa cggcacactg cccctgtcgc acatgtaaat cgatagatcc taatcaacct
     4801 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4861 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4921 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4981 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5041 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5101 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5161 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5221 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5281 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5341 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     5401 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5461 gctaattcac tcccaacgaa gacaagatca cctgcaggac aggcgcgccc tgctttttgc
     5521 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5701 ctctagcacc cgggcgatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     5761 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5821 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5881 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5941 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6001 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6061 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6121 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6181 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6241 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6301 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6361 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6421 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6481 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6541 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6601 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6661 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6721 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6781 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6841 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6961 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7021 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7081 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7141 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7201 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7261 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7321 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7801 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7861 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7921 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7981 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8041 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     8101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8161 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     8221 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     8281 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     8341 acttagtagt
//
