LOCUS       sequence-275824-        8602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible polycistronic lentiviral vector expressing mouse Sox2,
            cMyc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002."
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-SM
FEATURES             Location/Qualifiers
     source          1..8602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3651..3710
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    5044..5632
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5097..5117)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5515..5526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5780..5960
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6007..6025)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6093..6197
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6198..6266,6267..7058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6416..6435)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7229..7817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7971..7988
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8228..8249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8264..8269,8270..8287,8288..8294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8302..8318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8307..8329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8326..8342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8326..8342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8392..8411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgtat aacatgatgg
     2701 agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc ggaggaggca
     2761 acgccacggc ggcggcaacc ggcggcaacc agaagaacag cccggaccgc gtcaagaggc
     2821 ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc caggagaacc
     2881 ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa cttttgtccg
     2941 agaccgagaa gcggccgttc atcgacgagg ccaagcggct gcgcgctctg cacatgaagg
     3001 agcacccgga ttataaatac cggccgcggc ggaaaaccaa gacgctcatg aagaaggata
     3061 agtacacgct tcccggaggc ttgctggccc ccggcgggaa cagcatggcg agcggggttg
     3121 gggtgggcgc cggcctgggt gcgggcgtga accagcgcat ggacagctac gcgcacatga
     3181 acggctggag caacggcagc tacagcatga tgcaggagca gctgggctac ccgcagcacc
     3241 cgggcctcaa cgctcacggc gcggcacaga tgcaaccgat gcaccgctac gacgtcagcg
     3301 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3361 gcatgtccta ctcgcagcag ggcacccccg gtatggcgct gggctccatg ggctctgtgg
     3421 tcaagtccga ggccagctcc agcccccccg tggttacctc ttcctcccac tccagggcgc
     3481 cctgccaggc cggggacctc cgggacatga tcagcatgta cctccccggc gccgaggtgc
     3541 cggagcccgc tgcgcccagt agactgcaca tggcccagca ctaccagagc ggcccggtgc
     3601 ccggcacggc cattaacggc acactgcccc tgtcgcacat gggtagtggg caatgtacta
     3661 actacgcttt gttgaaactc gctggcgatg ttgaaagtaa ccccggtcct atgcccctca
     3721 acgtgaactt caccaacagg aactatgacc tcgactacga ctccgtacag ccctatttca
     3781 tctgcgacga ggaagagaat ttctatcacc agcaacagca gagcgagctg cagccgcccg
     3841 cgcccagtga ggatatctgg aagaaattcg agctgcttcc caccccgccc ctgtccccga
     3901 gccgccgctc cgggctctgc tctccatcct atgttgcggt cgctacgtcc ttctccccaa
     3961 gggaagacga tgacggcggc ggtggcaact tctccaccgc cgatcagctg gagatgatga
     4021 ccgagttact tggaggagac atggtgaacc agagcttcat ctgcgatcct gacgacgaga
     4081 ccttcatcaa gaacatcatc atccaggact gtatgtggag cggtttctca gccgctgcca
     4141 agctggtctc ggagaagctg gcctcctacc aggctgcgcg caaagacagc accagcctga
     4201 gccccgcccg cgggcacagc gtctgctcca cctccagcct gtacctgcag gacctcaccg
     4261 ccgccgcgtc cgagtgcatt gacccctcag tggtctttcc ctacccgctc aacgacagca
     4321 gctcgcccaa atcctgtacc tcgtccgatt ccacggcctt ctctccttcc tcggactcgc
     4381 tgctgtcctc cgagtcctcc ccacgggcca gccctgagcc cctagtgctg catgaggaga
     4441 caccgcccac caccagcagc gactctgaag aagagcaaga agatgaggaa gaaattgatg
     4501 tggtgtctgt ggagaagagg caaacccctg ccaagaggtc ggagtcgggc tcatctccat
     4561 cccgaggcca cagcaaacct ccgcacagcc cactggtcct caagaggtgc cacgtctcca
     4621 ctcaccagca caactacgcc gcacccccct ccacaaggaa ggactatcca gctgccaaga
     4681 gggccaagtt ggacagtggc agggtcctga agcagatcag caacaaccgc aagtgctcca
     4741 gccccaggtc ctcagacacg gaggaaaacg acaagaggcg gacacacaac gtcttggaac
     4801 gtcagaggag gaacgagctg aagcgcagct tttttgccct gcgtgaccag atccctgaat
     4861 tggaaaacaa cgaaaaggcc cccaaggtag tgatcctcaa aaaagccacc gcctacatcc
     4921 tgtccattca agcagacgag cacaagctca cctctgaaaa ggacttattg aggaaacgac
     4981 gagaacagtt gaaacacaaa ctcgaacagc ttcgaaactc tggtgcataa atcgatagat
     5041 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5101 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5161 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5221 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5281 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5341 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5401 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     5461 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5521 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5581 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     5641 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5701 gggactggaa gggctaattc actcccaacg aagacaagat cacctgcagg acaggcgcgc
     5761 cctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5821 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5881 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5941 agtgtggaaa atctctagca cccgggcgat taaggaaagg gctagatcat tcttgaagac
     6001 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6061 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6121 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6181 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6241 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6301 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6361 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6421 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     6481 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6541 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6601 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6661 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6721 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6781 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6841 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6901 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6961 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7021 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7081 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7141 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7201 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7261 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7321 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7621 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7801 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7861 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7921 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7981 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8041 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
     8101 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8161 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8221 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     8281 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8341 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8401 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     8461 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     8521 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     8581 tatgtactac aaacttagta gt
//
