LOCUS       sequence-275826-        6190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length human Tafazzin with codon modified for 
            cloning. Transgene flanked by AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Wang S, Li Y, Xu Y, Ma Q, Lin Z, Schlame M, Bezzerides VJ, Strathdee
            D, Pu WT
  TITLE     AAV Gene Therapy Prevents and Reverses Heart Failure in A Murine 
            Knockout Model of Barth Syndrome.
  JOURNAL   Circ Res. 2020 Mar 9. doi: 10.1161/CIRCRESAHA.119.315956.
  PUBMED    32146862
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Mar 9. doi: 10.1161/CIRCRESAHA.119.315956."
COMMENT     Sequence Label: pCAG/hTAZ-FL
FEATURES             Location/Qualifiers
     source          1..6190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        430..702
                     /label=chicken beta-actin promoter
     primer_bind     981..1003
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1065..1084
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1138..1270
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    2179..2767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2232..2252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2650..2661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2810..2931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2875..2894
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3093..3222
                     /label=AAV2 ITR
     primer_bind     complement(3500..3516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3500..3516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3513..3535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3548..3554,3555..3572,3573..3578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(3903..4491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3983..4002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4662..5453,5454..5522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5285..5304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5523..5627)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5653..6108)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5790..5811)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6002..6021
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   join(6180..6190,1..119)
                     /label=AAV2 ITR
ORIGIN
        1 ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt
      121 gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta ggaagagtac
      181 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      241 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      301 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      361 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      421 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
      481 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
      541 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      601 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      661 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      721 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      781 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      841 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      901 gagggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg gctgccttcg
      961 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     1021 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     1081 tgtgctgtct catcattttg gcaaagaatt ggatccccgg gtaccggtct ctgcagagta
     1141 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag
     1201 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt
     1261 ctctccacag ggcggccgcg ggtggggatg cctctgcacg tgaagtggcc gttccccgcg
     1321 gtgccgccgc tcacgtggac cctcgccagc agcgtcgtca tgggcttggt gggcacctac
     1381 agctgcttct ggaccaagta catgaaccac ctgaccgtgc acaacaggga ggtgctgtac
     1441 gagctcatcg agaagcgagg ccccgccacg cccctcatca ccgtgtccaa tcaccagtcc
     1501 tgcatggacg accctcatct ctgggggatt ctgaaactcc gccacatctg gaacctgaag
     1561 ttgatgcgtt ggacccctgc tgctgctgac atctgcttca ccaaggagct acactcccac
     1621 ttcttcagct tgggcaagtg tgtgcctgtg tgccgaggag cagaattttt ccaagcagag
     1681 aatgagggga aaggtgtact agacacaggc aggcacatgc caggtgctgg aaaaagaaga
     1741 gagaaaggag atggcgtcta ccagaagggg atggacttca ttttggagaa gctcaaccac
     1801 ggggactggg tgcatatctt cccagaaggg aaagtgaaca tgagttccga atttctgcgt
     1861 ttcaagtggg gaatcgggcg cctgattgct gagtgtcatc tcaaccccat catcctaccc
     1921 ctgtggcatg tcggaatgaa tgacgtcctt cctaacagtc cgccctactt cccccgcttt
     1981 ggacagaaaa tcactgtgct gatcgggaag cccttcagtg ccctgcctgt actcgagcgg
     2041 ctccgagcgg agaacaagtc ggctgtggag atgcggaaag ccctgacgga cttcattcaa
     2101 gaggaatttc agcatctgaa gactcaggca gagcagctcc acaaccacct ccagccaggg
     2161 agatagaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2221 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2281 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2341 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2401 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2461 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2521 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2581 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2641 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2701 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2761 tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt
     2821 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2881 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2941 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     3001 taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga
     3061 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     3121 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3181 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
     3241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3361 cggttatcca cagaatcagg ggataacgca ataaagcatt tttttcactg cattctagtt
     3421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     3481 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3541 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgca ctgaggccgg
     3601 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3661 gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5641 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5761 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5821 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5881 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5941 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     6001 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6061 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     6121 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6181 tgcgcgctcg
//
