LOCUS       sequence-27583-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kaelin WG, Krek W, Sellers WR, DeCaprio JA, Ajchenbaum F, Fuchs CS, 
            Chittenden T, Li Y, Farnham PJ, Blanar MA
  TITLE     Expression cloning of a cDNA encoding a retinoblastoma-binding 
            protein with E2F-like properties.
  JOURNAL   Cell. 1992 Jul 24. 70(2):351-64.
  PUBMED    1638635
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1992 
            Jul 24. 70(2):351-64."
COMMENT     Sequence Label: Rb379-928/C706F-pGex2TK
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(231..253)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(398..416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgccttnata ttgaaggatc agatgaagca gatggaagta aacatctccc aggagagtcc
       61 aaatttcagc agaaactggc agaaatgact tctactcgaa cacgaatgca aaagcagaaa
      121 atgaatgata gcatggatac ctcaaacaag gaagagaaat gaggatcccc gggaattcat
      181 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      241 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      301 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
      361 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      421 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgccttctt cccttttttt gcggcatttt gccttgctgt ttttgctcac
      661 ccagaaacgc tngtgaanta aaanatgntg aanaaaatca gtngggtgca cgagtggtta
      721 catcgaactg gatctcacag cgtaaatcnt gagntttcgc ccgaaaacgt ttccatgaga
      781 cnctttaagt ctgcttgtgc cgtatntccn tgtgacncgg cagacatcgt ccccncctat
      841 ccnatgctgt gatcccatcn aaa
//
