LOCUS       sequence-275832-       14928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein plk4 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-plk4
FEATURES             Location/Qualifiers
     source          1..14928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2708..4633)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGXGGGGGXGGXGXGGGA
                     GGXGGAGAGGGAGGGGGGGGGGXGGGXGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5068..5086)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5154..5258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5259..5327,5328..6119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5477..5496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6290..6878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6779..6798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7067..7087)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7085..7382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7459..8058
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7459..7478)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7955..7975
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8149..8283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8421..8440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8717..8978
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9095..9667
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9675..9694
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9722..9741
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9722..9740
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9751..10371
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9824..9843)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10413..10534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10589..10968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10969..11172
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11123..11143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11174..11192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11195..11213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11223..11247
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11347..11370
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11371..11388
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11938..11961
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11974..11998
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      13731..13740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(join(14918..14928,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     3961 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     4021 ctgctcctgc ccctcctcct gctcctgccc ctcctscccc tcctscccct cctcctsctc
     4081 ctccccctcc tccccctccy cctcctcctg cycctcctcc tgctcctgcc cctcctsccc
     4141 ctcctgcccc tcctccysct cctsccccyc ctsccccccc tcctcctcct scccctcctc
     4201 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     4261 ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     4321 ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg
     4381 tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc
     4441 ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc
     4501 ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc
     4561 cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc
     4621 cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta gacgacgctc
     4681 agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct
     4741 tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg
     4801 tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg
     4861 tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat
     4921 ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg
     4981 gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca
     5041 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5101 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5221 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5341 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5401 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5461 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     5521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5581 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5641 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5701 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     5821 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6061 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6421 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6901 tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc
     6961 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     7021 tggggaaggc catccagcct cgcgtcgaac tagatgatcc ggctgtggaa tgtgtgtcag
     7081 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7141 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7201 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7261 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7321 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7381 ggagggtgac cgccacgacc ggtgccgcca ccatcccctg acccacgccc ctgacccctc
     7441 acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7501 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7561 cacaccgtcg accccgaccg ccacatcgaa cgcgtcaccg agctgcaaga actcttcctc
     7621 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7681 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7741 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7801 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7861 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7921 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7981 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8041 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     8101 cgacccggtc cgacgcggcc cgacgggtcc gaggggggtc gacctcgaaa cttgtttatt
     8161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8281 atctgatccg aaccccttcc tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     8341 cgggcaacgt tgttgccatt gctcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     8401 tccgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8461 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8521 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8581 cttcgcgatg tacgggccag atatacgcgt atctgagggg actagggtgt gtttaggcga
     8641 aaagcggggc ttcggttgta cgcggttagg agtcccctca ggatatagta gtttcgcttt
     8701 tgcataggga gggggaaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     8761 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     8821 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     8881 gacgaaccac tgaattccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa
     8941 taaacgccat ttgaccattc accacattgg tgtgcacctc caagctggcc ggggatcctc
     9001 tagggcctct gagctattcc agaagtagtg aagaggcttt tttggaggcc taggcttttg
     9061 caaaaagctc cggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     9121 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     9181 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     9241 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     9301 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     9361 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     9421 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     9481 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     9541 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     9601 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     9661 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     9721 gtaatacgac tcactatagg gcgaattgat atgtctagat tagataaaag taaagtgatt
     9781 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     9841 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     9901 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     9961 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
    10021 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
    10081 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
    10141 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
    10201 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
    10261 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
    10321 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc
    10381 ggatcccggg aattcagatc ttattaaagc agaacttgtt tattgcagct tataatggtt
    10441 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10501 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggtcgaat ccatggcgca
    10561 gaactggtag gtatggaaga tccgcgttga cattgattat tgactagtta ttaatagtaa
    10621 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    10681 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    10741 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    10801 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    10861 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    10921 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    10981 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    11041 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    11101 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    11161 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
    11221 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
    11281 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact taagcttacc
    11341 accatggatt ataaagacga tgatgacaag caccaccacc accaccacga caaggattgt
    11401 gaaatgaaac gcaccacact ggacagccct ttggggaagc tggagctgtc tggttgtgag
    11461 cagggtctgc acgaaataat ttttctgggc aaggggacgt ctgcagctga tgccgtggag
    11521 gtcccagccc ccgctgcggt tctcggaggt ccggagcccc tgatgcaggc tacagcctgg
    11581 ctgaatgcct atttccacca gcccgaggct atcgaagagt tccccgtgcc ggctcttcac
    11641 catcccgttt tccagcaaga gtcgttcacc agacaggtgt tatggaagct gctgaaggtt
    11701 gtgaaattcg gagaagtgat ttcttactct cacttagcag ccctggcagg caaccccgca
    11761 gccacggcag cagtgaagac ggcactgagt ggcaatcctg tccctatcct gatcccgtgc
    11821 cacagagtgg tccaggggga tttggatgtg ggcggttacg agggtggact ggccgtgaag
    11881 gaatggcttc tggcccatga aggccaccgg ttggggaagc caggcttggg attcgaactg
    11941 gaggtgctgt tccagggccc cggtaccgcc atcacaagtt tgtacaaaaa agcaggctta
    12001 atggcgacct gcatcgggga gaagatcgag gattttaaag ttggaaatct gcttggtaaa
    12061 ggatcatttg ctggtgtcta cagagctgag tccattcaca ctggtttgga agttgcaatc
    12121 aaaatgatag ataagaaagc catgtacaaa gcaggaatgg tacagagagt ccaaaatgag
    12181 gtgaaaatac attgccaatt gaaacatcct tctatcttgg agctttataa ctattttgaa
    12241 gatagcaatt atgtgtatct ggtattagaa atgtgccata atggagaaat gaacaggtat
    12301 ctaaagaata gagtgaaacc cttctcagaa aatgaagctc gacacttcat gcaccagatc
    12361 atcacaggga tgttgtatct tcattctcat ggtatactac accgggacct cacactttct
    12421 aacctcctac tgactcgtaa tatgaacatc aagattgctg attttgggct ggcaactcaa
    12481 ctgaaaatgc cacatgaaaa gcactataca ttatgtggaa ctcctaacta catttcacca
    12541 gaaattgcca ctcgaagtgc acatggcctt gaatctgatg tttggtccct gggctgtatg
    12601 ttttatacat tacttatcgg gagaccaccc ttcgacactg acacagtcaa gaacacatta
    12661 aataaagtag tattggcaga ttatgaaatg ccaacttttt tgtcaataga ggccaaggac
    12721 cttattcacc agttacttcg tagaaatcca gcagatcgtt taagtctgtc ttcagtattg
    12781 gaccatcctt ttatgtcccg aaattcttca acaaaaagta aagatttagg aactgtggaa
    12841 gactcaattg atagtgggca tgccacaatt tctactgcaa ttacagcttc ttccagtacc
    12901 agtataagtg gtagtttatt tgacaaaaga agacttttga ttggtcagcc actcccaaat
    12961 aaaatgactg tatttccaaa gaataaaagt tcaactgatt tttcttcttc aggagatgga
    13021 aacagttttt atactcagtg gggaaatcaa gaaaccagta atagtggaag gggaagagta
    13081 attcaagatg cagaagaaag gccacattct cgataccttc gtagagctta ttcctctgat
    13141 agatctggca cttctaatag tcagtctcaa gcaaaaacat atacaatgga acgatgtcac
    13201 tcagcagaaa tgctttcagt gtccaaaaga tcaggaggag gtgaaaatga agagaggtac
    13261 tcacccacag acaacaatgc caacattttt aacttcttta aagaaaagac atccagtagt
    13321 tctggatctt ttgaaagacc tgataacaat caagcactct ccaatcatct ttgtccagga
    13381 aaaactcctt ttccatttgc agacccgaca cctcagactg aaaccgtaca acagtggttt
    13441 gggaatctgc aaataaatgc tcatttaaga aaaactactg aatatgacag catcagccca
    13501 aaccgggact tccagggcca tccagatttg cagaaggaca catcaaaaaa tgcctggact
    13561 gatacaaaag tcaaaaagaa ctctgatgct tctgataatg cacattctgt aaaacagcaa
    13621 aataccatga aatatatgac tgcacttcac agtaaacctg agataatcca acaagaatgt
    13681 gtttttggct cagatcctct ttctgaacag agcaagacta ggggtatgga gccaccatgg
    13741 ggttatcaga atcgtacatt aagaagcatt acatctccgt tggttgctca caggttaaaa
    13801 ccaatcagac agaaaaccaa aaaggctgtg gtgagcatac ttgattcaga ggaggtgtgt
    13861 gtggagcttg taaaggagta tgcatctcaa gaatatgtga aagaagttct tcagatatct
    13921 agtgatggaa atacgatcac tatttattat ccaaatggtg gtagaggttt tcctcttgct
    13981 gatagaccac cctcacctac tgacaacatc agtaggtaca gctttgacaa tttaccagaa
    14041 aaatactggc gaaaatatca atatgcttcc aggtttgtac agcttgtaag atctaaatct
    14101 cccaaaatca cttattttac aagatatgct aaatgcattt tgatggagaa ttctcctggt
    14161 gctgattttg aggtttggtt ttatgatggg gtaaaaatac acaaaacaga agatttcatt
    14221 caggtgattg aaaagacagg gaagtcttac actttaaaaa gtgaaagtga agttaatagc
    14281 ttgaaagagg agataaaaat gtatatggac catgctaatg agggtcatcg tatttgttta
    14341 gcactggaat ccataatttc agaagaggaa aggaaaacta ggagtgctcc ctttttccca
    14401 ataatcatag gaagaaaacc tggtagtact agttcaccta aggccttatc acctcctcct
    14461 tctgtggatt caaattaccc aacgagagat agagcatctt tcaacagaat ggtcatgcat
    14521 agtgctgctt ctccaacaca ggcaccaatc cttaatccct ctatggttac aaatgaagga
    14581 cttggtctta caactacagc ttctggaaca gacatctctt ctaatagtct aaaagattgt
    14641 cttcctaaat cagcacaact tttgaaatct gtttttgtga aaaatgttgg ttgggctaca
    14701 cagttaacta gtggagctgt gtgggttcag tttaatgatg ggtcccagtt ggttgtgcag
    14761 gcaggagtgt cttctatcag ttatacctca ccaaatggtc aaacaactag gtatggagaa
    14821 aatgaaaaat taccagacta catcaaacag aaattacagt gtctgtcttc catccttttg
    14881 atgttttcta atccgactcc taattttcat taatgatacc cagctttc
//
