LOCUS       sequence-275834-       13374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..13374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2708..4636)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGGAGAGGAGGAGAGGAGGXGAGGAGGAGAGGGAGGAGA
                     GGGAGGAGAGGAGGGGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGR
                     GGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRP
                     PPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGG
                     RRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTS
                     LYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL
                     KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGD
                     EGEEGQE"
     primer_bind     complement(5071..5089)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5157..5261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5262..5330,5331..6122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5480..5499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6293..6881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6782..6801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7070..7090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7088..7385
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7462..8061
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7462..7481)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7958..7978
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8152..8286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8424..8443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8720..8981
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9098..9670
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9678..9697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9725..9744
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9725..9743
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9754..10374
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9827..9846)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10416..10537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10592..10971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10972..11175
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11126..11146
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11177..11195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11198..11216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11226..11250
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11350..11373
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11374..11391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11941..11964
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11977..12001
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13364..13374,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctsctcctc cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tbcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
     3961 ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc
     4021 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcs cctcctcctg
     4081 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4141 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4201 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     4261 ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     4321 ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt
     4381 gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat
     4441 gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc
     4501 ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg
     4561 agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg
     4621 gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg
     4681 ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc
     4741 ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc
     4801 tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt
     4861 gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg
     4921 gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact
     4981 ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg
     5041 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5101 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     5521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     5821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6421 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6901 ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct
     6961 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     7021 taatggggaa ggccatccag cctcgcgtcg aactagatga tccggctgtg gaatgtgtgt
     7081 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7141 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7201 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7261 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7321 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7381 tttggagggt gaccgccacg accggtgccg ccaccatccc ctgacccacg cccctgaccc
     7441 ctcacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7501 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7561 cgccacaccg tcgaccccga ccgccacatc gaacgcgtca ccgagctgca agaactcttc
     7621 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7681 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7741 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7801 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7861 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7921 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7981 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8041 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     8101 gcacgacccg gtccgacgcg gcccgacggg tccgaggggg gtcgacctcg aaacttgttt
     8161 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8221 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8281 tggatctgat ccgaacccct tcctcgacca attctcatgt ttgacagctt atcatcgcag
     8341 atccgggcaa cgttgttgcc attgctcgac caattctcat gtttgacagc ttatcatcgc
     8401 agatccgtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8461 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8521 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8581 ctgcttcgcg atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg
     8641 cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc
     8701 ttttgcatag ggagggggaa atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     8761 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     8821 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     8881 ttggacgaac cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8941 caataaacgc catttgacca ttcaccacat tggtgtgcac ctccaagctg gccggggatc
     9001 ctctagggcc tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt
     9061 ttgcaaaaag ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg
     9121 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     9181 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     9241 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     9301 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     9361 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     9421 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     9481 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat
     9541 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     9601 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt
     9661 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     9721 attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa aagtaaagtg
     9781 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
     9841 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg
     9901 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct
     9961 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct
    10021 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag
    10081 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat
    10141 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa
    10201 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta
    10261 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc
    10321 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac
    10381 agcggatccc gggaattcag atcttattaa agcagaactt gtttattgca gcttataatg
    10441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg aatccatggc
    10561 gcagaactgg taggtatgga agatccgcgt tgacattgat tattgactag ttattaatag
    10621 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    10681 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    10741 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    10801 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    10861 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    10921 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    10981 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    11041 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    11101 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    11161 tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt
    11221 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
    11281 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt
    11341 accaccatgg attataaaga cgatgatgac aagcaccacc accaccacca cgacaaggat
    11401 tgtgaaatga aacgcaccac actggacagc cctttgggga agctggagct gtctggttgt
    11461 gagcagggtc tgcacgaaat aatttttctg ggcaagggga cgtctgcagc tgatgccgtg
    11521 gaggtcccag cccccgctgc ggttctcgga ggtccggagc ccctgatgca ggctacagcc
    11581 tggctgaatg cctatttcca ccagcccgag gctatcgaag agttccccgt gccggctctt
    11641 caccatcccg ttttccagca agagtcgttc accagacagg tgttatggaa gctgctgaag
    11701 gttgtgaaat tcggagaagt gatttcttac tctcacttag cagccctggc aggcaacccc
    11761 gcagccacgg cagcagtgaa gacggcactg agtggcaatc ctgtccctat cctgatcccg
    11821 tgccacagag tggtccaggg ggatttggat gtgggcggtt acgagggtgg actggccgtg
    11881 aaggaatggc ttctggccca tgaaggccac cggttgggga agccaggctt gggattcgaa
    11941 ctggaggtgc tgttccaggg ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc
    12001 ttagtgcgtg agtgcatctc catccacgtt ggccaggctg gtgtccagat tggcaatgcc
    12061 tgctgggagc tctactgcct ggaacacggc atccagcccg atggccagat gccaagtgac
    12121 aagaccattg ggggaggaga tgactccttc aacaccttct tcagtgagac gggcgctggc
    12181 aagcacgtgc cccgggctgt gtttgtagac ttggaaccca cagtcattga tgaagttcgc
    12241 actggcacct accgccagct cttccaccct gagcagctca tcacaggcaa ggaagatgct
    12301 gccaataact atgcccgagg gcactacacc attggcaagg agatcattga ccttgtgttg
    12361 gaccgaattc gcaagctggc tgaccagtgc accggtcttc agggcttctt ggttttccac
    12421 agctttggtg ggggaactgg ttctgggttc acctccctgc tcatggaacg tctctcagtt
    12481 gattatggca agaagtccaa gctggagttc tccatttacc cagcacccca ggtttccaca
    12541 gctgtagttg agccctacaa ctccatcctc accacccaca ccaccctgga gcactctgat
    12601 tgtgccttca tggtagacaa tgaggccatc tatgacatct gtcgtagaaa cctcgatatc
    12661 gagcgcccaa cctacactaa ccttaaccgc cttattagcc agattgtgtc ctccatcact
    12721 gcttccctga gatttgatgg agccctgaat gttgacctga cagaattcca gaccaacctg
    12781 gtgccctacc cccgcatcca cttccctctg gccacatatg cccctgtcat ctctgctgag
    12841 aaagcctacc atgaacagct ttctgtagca gagatcacca atgcttgctt tgagccagcc
    12901 aaccagatgg tgaaatgtga ccctcgccat ggtaaataca tggcttgctg cctgttgtac
    12961 cgtggtgacg tggttcccaa agatgtcaat gctgccattg ccaccatcaa aaccaagcgc
    13021 agcatccagt ttgtggattg gtgccccact ggcttcaagg ttggcatcaa ctaccagcct
    13081 cccactgtgg tgcctggtgg agacctggcc aaggtacaga gagctgtgtg catgctgagc
    13141 aacaccacag ccattgctga ggcctgggct cgcctggacc acaagtttga cctgatgtat
    13201 gccaagcgtg cctttgttca ctggtacgtg ggtgagggga tggaggaagg cgagttttca
    13261 gaggcccgtg aagatatggc tgcccttgag aaggattatg aggaggttgg tgtggattct
    13321 gttgaaggag agggtgagga agaaggagag gaatactaat gatacccagc tttc
//
