LOCUS       sequence-275856-       14325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression vector for direct cloning of synthetic trans-acting
            siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+].
            Contains AtMIR173 for syntasi expression in any plant species..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Lopez-Dolz L, Spada M, Daros JA, Carbonell A
  TITLE     Fine-tune control of targeted RNAi efficacy by plant artificial 
            small RNAs.
  JOURNAL   Nucleic Acids Res. 2020 May 12. pii: 5836195. doi: 
            10.1093/nar/gkaa343.
  PUBMED    32396204
REFERENCE   2  (bases 1 to 14325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 May 12. pii: 5836195. doi: 10.1093/nar/gkaa343."
COMMENT     Sequence Label: pMDC32B-AtTAS1c-D2-B/c-AtMIR173
FEATURES             Location/Qualifiers
     source          1..14325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3897)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4326..4955)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6256..6280
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6468..6490
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6482..6499
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6483..6499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6925..7269
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7329..7353
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(7796..7801,7802..7819,7820..7826)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7880..8539
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7946..7965)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8881..9186
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9065..9084
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9841..9865)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        10606..10950
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    11010..11034
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11579..11603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11935..11951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11935..11951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11948..11970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11959..11975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11983..11989,11990..12007,12008..12013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12028..12049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12240..12916
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             12983..14008
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    14049..14223
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14301..14325
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaagagaact tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     3901 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     3961 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     4021 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     4081 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     4141 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     4201 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     4261 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     4321 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     4381 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     4441 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     4501 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     4561 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     4621 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     4681 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     4741 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     4801 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     4861 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     4921 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     4981 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     5041 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     5101 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     5161 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     5221 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     5281 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     5341 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     5401 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     5461 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     5521 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     5581 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     5641 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     5701 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     5761 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     5821 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     5881 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     5941 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     6001 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     6061 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     6121 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     6181 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     6241 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     6301 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     6361 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6421 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6481 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcaacatgg tggagcacga
     6541 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
     6601 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
     6661 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
     6721 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     6781 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     6841 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
     6901 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
     6961 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
     7021 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
     7081 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     7141 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     7201 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     7261 ggagaggacc tcgactctag aggatccccg ggtaccgggc cccccctcga ggcgcgccaa
     7321 gctatcaaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accaaaccta
     7381 aacctaaacg gctaagcccg acgtcaaata ccaaaaagag aaaaacaaga gcgccgtcaa
     7441 gctctgcaaa tacgatctgt aagtccatct taacacaaaa gtgagatggg ttcttagatc
     7501 atgttccgcc gttagatcga gtcatggtct tgtctcatag aaaggtactt tcgtttactt
     7561 cttttgagta tcgagtagag cgtcgtctat agttagtttg agattgcgtt tgtcagaagt
     7621 taggttcaat gtcccggtcc aattttcacc agccatgtgt cagtttcgtt ccttcccgtc
     7681 ctcttctttg atttcgttgg gttacggatg ttttcgagat gaaacagcat tgttttgttg
     7741 tgatttttct ctacaagcga atagaccatt taagagacca ttaggcaccc caggctttac
     7801 actttatgct tccggctcgt ataatgtgtg gattttgagt taggagccgt cgagattttc
     7861 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     7921 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     7981 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     8041 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggagttccg
     8101 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     8161 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     8221 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     8281 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     8341 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     8401 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     8461 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     8521 gtggcagggc ggggcgtaaa cgcgtggagc cggcttacta aaagccagat aacagtatgc
     8581 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     8641 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     8701 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     8761 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     8821 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
     8881 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     8941 cagagtgata ttattgacac gcccggccga cggatggtga tccccctggc cagtgcacgt
     9001 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     9061 tggcgcatga tgaccaccga tatggccagt gtgccggttt ccgttatcgg ggaagaagtg
     9121 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     9181 atataaatgt caggctccct tatacacagc cagtctgcac ctcgacggtc tcatcggtgg
     9241 atcttagaaa attattctaa gtccaacata gcgtattcta agttcaacat atcgacgaac
     9301 tagaaaagac attggacata ttccaggata tgcaaaagaa aacaatgaat attgttttga
     9361 atgtgttcaa gtaaatgaga ttttcaagtc gtctaaagaa cagttgctaa tacagttact
     9421 tatttcaata aataattggt tctaataata caaaacatat tcgaggatat gcagaaaaaa
     9481 agatgtttgt tattttgaaa agcttgagta gtttctctcc gaggtgtagc gaagaagcat
     9541 catctacttt gtaatgtaat tttctttatg ttttcacttt gtaattttat ttgtgttaat
     9601 gtaccatggc cgatatcggt tttattgaaa gaaaatttat gttacttctg ttttggcttt
     9661 gcaatcagtt atgctagttt tcttataccc tttcgtaagc ttcctaagga atcgttcatt
     9721 gatttccact gcttcattgt atattaaaac tttacaactg tatcgaccat catataattc
     9781 tgggtcaaga gatgaaaata gaacaccaca tcgtaaagtg aaataagggt gggcgcgccg
     9841 acccagcttt cttgtacaaa gtggttcgat aattcttaat taactagttc tagagcggcc
     9901 gccaccgcgg tggagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     9961 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
    10021 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
    10081 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
    10141 ggataaatta tcgcgcgcgg tgtcatctat gttactgaat tcaagcttgg cgtgcctgca
    10201 ggtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc
    10261 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg
    10321 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
    10381 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
    10441 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
    10501 cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta
    10561 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
    10621 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
    10681 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
    10741 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
    10801 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
    10861 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
    10921 ataaggaagt tcatttcatt tggagaggac ctcgactcta gaggatcccc gggtaccggg
    10981 ccccccctcg aggcgcgcca agctatcaaa caagtttgta caaaaaagca ggctccgcgg
    11041 cccacccttc accataatta gcaagtaata aggaataaaa tcctgtaatt atagctgaaa
    11101 aaggaaaaat atttggagtc cgtcagattc gaatctgaac aaagcataaa aaagtcaaca
    11161 aaacttaaag cggcggactc atcgtaatct cagcccaata ccctattttc ctctccccta
    11221 tataaatact ttcttcttct actgatcttc ttctcacaaa taaacccaaa tatatcaatc
    11281 tactgtgttg gtgattaagt actttcgctt gcagagagaa atcacagtgg tcaaaaaagt
    11341 tgtagttttc ttaaagtctc tttcctctgt gattctctgt gtaagcgaaa gagcttgctc
    11401 cctaaactta tctctctgat gatttaatgt tagagatctt cgtaaatcta tgtgtttgat
    11461 agatctgatg cgttttttga gttgatgatt tgattatttt tcactggaaa gtatctcatt
    11521 agggtaacga taatgtttta tggatttggt tgtataacag ataagggtgg gcgcgccgac
    11581 ccagctttct tgtacaaagt ggttcgataa ttcttaatta actagttcta gagcggccgc
    11641 caccgcggtg gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
    11701 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
    11761 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
    11821 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
    11881 ataaattatc gcgcgcggtg tcatctatgt tactgaattc gaattcgtaa tcatgtcata
    11941 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    12001 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    12061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    12121 acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac
    12181 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
    12241 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
    12301 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
    12361 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
    12421 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
    12481 gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
    12541 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
    12601 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
    12661 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
    12721 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
    12781 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
    12841 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
    12901 ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg
    12961 cagatccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
    13021 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
    13081 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
    13141 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
    13201 gattccggaa gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg
    13261 ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca
    13321 accggtcgcg gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
    13381 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
    13441 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
    13501 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
    13561 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
    13621 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
    13681 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
    13741 tccggagctt gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca
    13801 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
    13861 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
    13921 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    13981 cagcactcgt ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca
    14041 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
    14101 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
    14161 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
    14221 atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
    14281 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgcca
//
