LOCUS       sequence-275866-        7841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tunable and temporal expression control of NFIL3 and nuclear eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  TITLE     Xlone Puro plasmids for inducible gene expression in mammal cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: XLone-BSD NFIL3 P2A eGFP NLS
FEATURES             Location/Qualifiers
     source          1..7841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1790..1846
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1847..1849,1850..1852,1853..2563)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1847..1849,1850..1852,1853..2563)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1892..1913)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2153..2172)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2500..2521
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2564..2584
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2803..2937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3213..3247
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3258..3275)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3258..3274)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3267..3289)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3483..3502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3602..3624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3662..3680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3748..3852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3853..3921,3922..4713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4071..4090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4884..5472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5373..5392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5626..5643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5760..5781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5796..5801,5802..5819,5820..5826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5834..5850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5839..5861
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5858..5874
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5858..5874
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5900..5962
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6138..6219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6336..6734)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(6735..6791)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6801..7544)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7545..7568)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7568..7577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7597..7808)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgccaccat gcagctgaga aaaatgcaga ccgtcaaaaa
      421 ggagcaggcg tctcttgatg ccagtagcaa tgtggacaag atgatggtcc ttaattctgc
      481 tttaacggaa gtgtcagaag actccacaac aggtgaggag ctgcttctca gtgaaggaag
      541 tgtggggaag aacaaatctt ctgcatgtcg gaggaaacgg gaattcattc ctgatgaaaa
      601 gaaagatgct atgtattggg aaaaaaggcg gaaaaataat gaagctgcca aaagatctcg
      661 tgagaagcgt cgactgaatg acctggtttt agagaacaaa ctaattgcac tgggagaaga
      721 aaacgccact ttaaaagctg agctgctttc actaaaatta aagtttggtt taattagctc
      781 cacagcatat gctcaagaga ttcagaaact cagtaattct acagctgtgt actttcaaga
      841 ttaccagact tccaaatcca atgtgagttc atttgtggac gagcacgaac cctcgatggt
      901 gtcaagtagt tgtatttctg tcattaaaca ctctccacaa agctcgctgt ccgatgtttc
      961 agaagtgtcc tcagtagaac acacgcagga gagctctgtg cagggaagct gcagaagtcc
     1021 tgaaaacaag ttccagatta tcaagcaaga gccgatggaa ttagagagct acacaaggga
     1081 gccaagagat gaccgaggct cttacacagc gtccatctat caaaactata tggggaattc
     1141 tttctctggg tactcacact ctcccccact actgcaagtc aaccgatcct ccagcaactc
     1201 cccgagaacg tcggaaactg atgatggtgt ggtaggaaag tcatctgatg gagaagacga
     1261 gcaacaggtc cccaagggcc ccatccattc tccagttgaa ctcaagcatg tgcatgcaac
     1321 tgtggttaaa gttccagaag tgaattcctc tgccttgcca cacaagctcc ggatcaaagc
     1381 caaagccatg cagatcaaag tagaagcctt tgataatgaa tttgaggcca cgcaaaaact
     1441 ttcctcacct attgacatga catctaaaag acatttcgaa ctcgaaaagc atagtgcccc
     1501 aagtatggta cattcttctc ttactccttt ctcagtgcaa gtgactaaca ttcaagattg
     1561 gtctctcaaa tcggagcact ggcatcaaaa agaactgagt ggcaaaactc agaatagttt
     1621 caaaactgga gttgttgaaa tgaaagacag tggctacaaa gtttctgacc cagagaactt
     1681 gtatttgaag caggggatag caaacttatc tgcagaggtt gtctcactca agagacttat
     1741 agccacacaa ccaatctctg cttcagactc tggggctagc ggcagcggcg ccactaactt
     1801 ctccctgttg aaacaagcag gggatgtcga agagaatccc gggccaatgg tgagcaaggg
     1861 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1921 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1981 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2041 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2101 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2161 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2221 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2281 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2341 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2401 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2461 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2521 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagccaaaga agaagcgtaa
     2581 ggtataagta caagtaagaa ttccgatcat attcaataac ccactagtag accacctccc
     2641 ctgcgagcta agctggacag ccaatgacgg gtaagagagt gacatttttc actaacctaa
     2701 gacaggaggg ccgtcagagc tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt
     2761 atcactccaa cctaagacag gcgcagcttc cgagggattt gagatccaga catgataaga
     2821 tacattgatg agtttggaca aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt
     2881 gaaatttgtg atgctattgc cttatttgta accattataa gctgcaataa acaagtttga
     2941 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     3001 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     3061 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     3121 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     3181 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     3241 tctagggtta agaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3301 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3361 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     3421 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3481 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3541 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3601 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3661 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3721 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3841 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3901 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3961 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4021 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4081 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4141 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4201 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4261 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4321 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4381 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4441 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4501 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4561 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4621 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4681 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4741 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4801 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5641 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5701 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5761 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5821 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     5881 tacgccaagg tcgacttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
     5941 tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
     6001 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
     6061 caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata
     6121 aagtaacaaa acttttagca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6241 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     6301 aaatgtggta tggctgatta tgatcctctg gagatttagc cctcccacac ataaccagag
     6361 ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct
     6421 ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg
     6481 cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca
     6541 gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca
     6601 ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag
     6661 atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac
     6721 aaaggcttgg ccatggggcc ggggttctcc tccacgtcgc cggcctgctt cagcaggctg
     6781 aagttggtgg cgccgctgcc cccggggagc atgtcaaggt caaaatcgtc aagagcgtca
     6841 gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat gtctaagtca
     6901 aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact ttagctgttt ctccaggcca
     6961 catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc
     7021 tcaattgctt gtttcagaag tgggggcata gaatcggtgg taggtgtctc tctttcctct
     7081 tttgctactt gatgctcctg ttcctccaat acgcagccca gtgtaaagtg gcccacggcg
     7141 gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa cgcgagctga
     7201 ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac tttagccccg
     7261 tcgcgatgtg agaggagagc acagcggtat gacttggcgt tgttccgcag aaagtcttgc
     7321 catgactcgc cttccagggg gcaggagtgg gtatgatgcc tgtccagcat ctcgattggc
     7381 agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg ctgctcaact
     7441 cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgactcc attgagtaat
     7501 tccagagcag agtttatgac tttgctcttg tccagtctag acatcttatc gtcatcgtct
     7561 ttgtaatcca tggtggcgga tcccgcgtca cgacacctgt gttctggcgg caaacccgtt
     7621 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     7681 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     7741 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     7801 ctctgcccac tgacgggcac cggagccact cgagtggaat t
//
