LOCUS       sequence-275884-        6221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HA-tagged TFDP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Krek W, Livingston DM, Shirodkar S
  TITLE     Binding to DNA and the retinoblastoma gene product promoted by 
            complex formation of different E2F family members.
  JOURNAL   Science. 1993 Dec 3. 262(5139):1557-60.
  PUBMED    8248803
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1993 Dec 3. 262(5139):1557-60."
COMMENT     Sequence Label: pCMV HA-DP-1
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             670..696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             670..696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             676..702
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(2180..2198)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2181..2198)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          2282..2347
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2477..2497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2769..2903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2806..2825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2860..2879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3754..3889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4384..4403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(4516..4974)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4653..4674)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4868..4887
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5098..5116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5184..5288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5289..5357,5358..6149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5507..5526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 accatggcct acccctacga cgtgcccgac tacgcctccc tcggatccac catggcaaaa
      721 gatgccagtc taattgaagc caacggagaa ctaaaggtct ttatagacca gaatcttagt
      781 cctgggaaag gtgtggtatc tcttgtagcc gtccacccgt ccacagtcaa cacacttggg
      841 aagcagcttt tgccaaaaac cttcggacag tccaatgtca atattacaca gcaagtggtg
      901 attggcacgc ctcagagacc ggcagcatcc aacactattg tggtaggaag cccacacact
      961 cccaacacgc attttgtgtc acagaaccag acgtctgact cctcaccttg gtctgctggg
     1021 aagcggaaca ggaagggcga gaagaatggc aagggcctgc ggcatttctc catgaaggtg
     1081 tgtgagaagg tgcagaggaa aggaaccacc tcctacaatg aggtggctga cgagctggtg
     1141 gcagagttca gcgctgccga caaccacatt ctaccaaacg aatcagctta tgaccagaag
     1201 aacatccggc ggcgtgtcta cgatgcctta aatgtgctaa tggccatgaa catcatctcc
     1261 aaggagaaga aggagatcaa atggatcggc ctgcccacca actcagctca ggagtgccag
     1321 aacttagagg tagagaggca gaggaggctg gagaggatca aacagaagca gtcacagctc
     1381 caggagctca tcctgcagca aattgccttc aagaacttgg tgcagagaaa tcgccaagct
     1441 gagcagcagg cccgcaggcc gcctcctccc aactctgtca tccacttgcc cttcatcatt
     1501 gtcaacacca gcaggaagac agtcattgac tgcagcatct ccaatgacaa atttgagtat
     1561 ctgtttaact ttgacaacac gtttgagatc cacgatgaca ttgaggtgct caagcgcatg
     1621 ggcatggcat gtgggctgga gtctggcaac tgctctgctg aagacctcaa ggtggccaga
     1681 agtttggtac caaaagctct agaaccatac gtgacagaaa tggctcaggg atccattggt
     1741 ggagtattcg tcacgacaac aggttctaca tccaatggca caaggctttc tgccagtgat
     1801 ttgagcaatg gtgcagatgg gatgctggcc acgagctcca atgggtctca gtacagcggc
     1861 tccagggtcg agacccctgt gtcctacgtt ggggaggatg atgacgacga tgatgacttt
     1921 aatgagaacg acgaggagga ttgattactc aacccgtaga cccctctccc cttcgaatca
     1981 gcttcaggaa aaacacgtat agaggaaaga aacttaaagt ggggctttct gttctttttg
     2041 gcctactccc aagaagatac ccgcgagttc tggagttaga tgtgcagctc caagtgagga
     2101 ggagtgtgct accgagctcg aattctgcag atatccatca cactggcggc cgctcgagca
     2161 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagaggatct ttgtgaagga
     2221 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     2281 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     2341 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     2401 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     2461 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     2521 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     2581 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     2641 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     2701 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2761 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2821 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2881 atgtatctta tcatgtctgg atcgatcccg ccatggtatc aacgccatat ttctatttac
     2941 agtagggacc tcttcgttgt gtaggtaccg ctgtattcct agggaaatag tagaggcacc
     3001 ttgaactgtc tgcatcagcc atatagcccc cgctgttcga tttacaaaca caggcacagt
     3061 actgacaaac ccatacacct cctctgaaat acccatagtt gctagggctg tctccgaact
     3121 cattacaccc tccaaagtca gagctgtaat ttcgccatca agggcagcga gggcttctcc
     3181 agataaaata gcttctgccg agagtcccgt aagggtagac acttcagcta atccctcgat
     3241 gaggtctact agaatagtca gtgcggctcc cattttgaaa attcacttac ttgatcagct
     3301 tcagaagatg gcggagggcc tccaacacag taattttcct cccgactctt aaaatagaaa
     3361 atgtcaagtc agttaagcag gaagtgacta actgaccgca gctggccgtg cgacatcctc
     3421 ttttaattag ttgctaggca actgccctcc agagggcagt gtggttttgc aagaggaagc
     3481 aaaaagcctc tccacccagg cctagaatgt ttccacccaa tcattactat gacaacagct
     3541 gtttttttta gtattaagca gaggccgggg gcccctggcc tccgcttact ctggagaaaa
     3601 agaagagagg cattgtagag gcttccagag gcaacttgtc aaaacaggac tgcttctatt
     3661 tctgtcacac tgtctggccc tgtcacaagg tccagcacct ccataccccc tttaataagc
     3721 agtttgggaa cgggtgcggg tcttactccg cccatcccgc ccctaactcc gcccagttcc
     3781 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3841 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3901 aaaaagctaa ttcggcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3961 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4021 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4081 ctctgtagca ccgcctacat acctcgctct gctgaagcca gttaccagtg gctgctgcca
     4141 gtggcgataa gtcgtgtctt accgggttgg actcaagaga tagttaccgg ataaggcgca
     4201 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4261 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4321 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4381 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4441 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcaag
     4501 ctagcttcta gctagaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     4561 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4621 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4681 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggccgcccac
     4741 tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4801 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4861 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4921 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtactatg
     4981 gttgctttga cgagcacgta taacgtgctt tcctcgttag aatcagagcg ggagctaaac
     5041 aggaggccga ttaaagggat tttagacagg aacggtacgc cagctggacc cgaaagggcc
     5101 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5161 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5221 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5281 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5341 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5401 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5461 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5521 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5581 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5641 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5701 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5761 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5821 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5881 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5941 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6001 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6061 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6121 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6181 agattgattt aaaacttcat ttttaatttc tctagcgcgt t
//
