LOCUS       sequence-275886-        7780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HA-tagged TFDP2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Lindeman GJ, Gaubatz S, Livingston DM, Ginsberg D
  TITLE     The subcellular localization of E2F-4 is cell-cycle dependent.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 May 13. 94(10):5095-100.
  PUBMED    9144196
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 May 13. 94(10):5095-100."
COMMENT     Sequence Label: pCMV HA-DP-2
FEATURES             Location/Qualifiers
     source          1..7780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        312..691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        692..895
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     846..866
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     892..916
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1007..1579
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1680..1706
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3013..3032)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3078..3133
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3132..3151)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3491..3507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3491..3507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3504..3526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3515..3531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3539..3545,3546..3563,3564..3569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3584..3605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3722..3739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3893..4481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3973..3992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4652..5443,5444..5512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5275..5294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5513..5617)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5685..5703
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5741..5763)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5863..5882
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6076..6098
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6090..6107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6091..6107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6518..6663
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             6720..7514
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6774..6793)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7384..7403
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7708..7727)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    join(7752..7780,1..19)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
       61 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
      121 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aagccctgcc
      181 atagccacgg gccccgtggg ttagggacgg cggatcgcgg ccctctagag agcttggccc
      241 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      481 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      541 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      601 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      661 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      721 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      781 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      841 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
      901 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      961 ccgggaccga tccagcctcc ggtcgaccga tcctgagaac ttcagggtga gtttggggac
     1021 ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt
     1081 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
     1141 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
     1201 attttctgta actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac
     1261 ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca
     1321 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
     1381 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
     1441 caactacacc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
     1501 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
     1561 cttcttctct ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
     1621 tggcaaagaa ttcctcgacg gacggtatcg ataagcttga tatcgaattc accatggcat
     1681 acccatacga tgttccagat tacgctagcg gatccatggt gaatgttgga ccccaaatga
     1741 ttataagcac accacagaga ctaaccagtt caggaagtgt tctgattggg agtccatata
     1801 cccctgcacc agcaatggtt actcagacac acatagcaga agctactggc tgggtccctg
     1861 gtgatagaaa acgggctaga aaatttatag actctgattt ttcagaaagt aaacgaagca
     1921 aaaaaggaga taaaaatggg aaaggcttga gacacttttc aatgaaagtg tgtgagaaag
     1981 ttcaacgaaa aggtacaaca tcgtacaatg aagtcgctga tgagctggtg tcagagttca
     2041 ccaattcaaa taaccatttg gctgctgatt cggcttatga tcagaagaac attaggcgaa
     2101 gagtttatga tgctttaaat gtgctaatgg caatgaacat aatttcaaag gaaaaaaaag
     2161 aaatcaagtg gattggcctg cctaccaatt ctgctcagga atgtcagaat ctggagatag
     2221 agaagcagag gcggatagaa cggataaagc agaagcgggc ccagctgcaa gaacttctcc
     2281 tacagcaaat cgctttcaaa aacctggtac agagaaatcg acaaaatgag cagcaaaacc
     2341 agggcccgcc ggctctgaac tctaccattc agctgccatt cataatcatc aatacaagca
     2401 gaaaaacagt catagattgc agcatctcca gtgacaagtt tgagtatctt ttcaattttg
     2461 acaacacctt tgagatccat gatgacatag aagtactaaa gcggatggga atgtcgtttg
     2521 gcctggagtc aggcaaatgc tctctggagg atctgaaact tgcgaaatcc ctggtgccaa
     2581 aggctttaga aggttatatc acagatatct ccacaggacc ttcttggtta aatcagggac
     2641 tacttctgaa ctctacccaa tcagtttcaa atttagacct gaccactggt gccaccttac
     2701 cccagtcaag tgtaaaccaa gggttatgct tggatgcaga agtggcctta gcaactgggc
     2761 agttcctggc cccaaacagt caccagtcca gcagtgcggc ctctcactgc tccgagtccc
     2821 gaggcgagac cccctgttcg ttcaatgatg aagatgagga agatgatgag gaggattcct
     2881 cctccccaga ataaagacaa gagaggatcc actagttcta gccgtcgagg aattcactcc
     2941 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     3001 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     3061 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3121 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     3181 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     3241 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     3301 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     3361 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     3421 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat
     3481 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3661 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3781 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4081 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4321 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4381 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4441 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4501 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4561 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4621 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4681 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4741 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4801 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4861 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4921 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4981 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5041 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5101 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5161 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5221 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5281 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5341 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5401 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5461 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5521 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5581 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5641 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     5701 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     5761 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     5821 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     5881 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5941 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6001 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6061 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttcacg
     6121 ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag
     6181 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     6241 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     6301 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     6361 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     6421 caggggatca agatcctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc
     6481 cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt
     6541 cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac
     6601 ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct
     6661 taacagcgtc aacagcgtgc cgcagatctg atcaagagac aggatgagga tcgtttcgca
     6721 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6781 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6841 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6901 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6961 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7021 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7081 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7141 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7201 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     7261 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7321 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7381 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7441 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7501 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7561 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7621 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7681 ccaccccggg agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     7741 ccgcgctatg acggcaataa aaagacagaa taaaacgcac
//
