LOCUS       sequence-275889-        5688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of E2F1-mt-luc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Zalvide J, Stubdal H, DeCaprio JA
  TITLE     The J domain of simian virus 40 large T antigen is required to 
            functionally inactivate RB family proteins.
  JOURNAL   Mol Cell Biol 1998 Mar;18(3):1408-15.
  PUBMED    9488456
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1998-03"; volume: "18(3)"; pages: "1408-15"
COMMENT     Sequence Label: pGL2-basic mt E2F-luciferase
FEATURES             Location/Qualifiers
     source          1..5688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    646..767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             958..2610
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1024..1042)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2851..2916
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3046..3066
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3491..3625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3767..3784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3938..4526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4018..4037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4697..5488,5489..5557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5320..5339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5558..5662)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgtggag gttttacttg ctttaaaaaa cctcccacac
      601 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatgg tactgtaact
      781 gagctaacat aacccgggag gtaccgagct ctgcaattgc tcgaccaact tgtgcaattg
      841 ctcgaccaac ttgtgcaatt gctcgaccaa cttgctcgag atctacttgg gcataaaagg
      901 cagagcaggg cagctgctgc ttacacttaa gcttggcatt ccggtactgt tggtaaaatg
      961 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc
     1021 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
     1081 tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt
     1141 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
     1201 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
     1261 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg
     1321 cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
     1381 aaattaccaa taatccagaa aattattatc atggattcta aaacggatta ccagggattt
     1441 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
     1501 gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct
     1561 actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat
     1621 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
     1681 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
     1741 gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa
     1801 attcaaagtg cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt
     1861 gacaaatacg atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa
     1921 gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg
     1981 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
     2041 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
     2101 ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat
     2161 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
     2221 gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta
     2281 attaaataca aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac
     2341 cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc
     2401 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
     2461 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
     2521 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
     2581 gccaagaagg gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt
     2641 cttagctatt gtaatactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     2701 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     2761 gcagaaattc gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     2821 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     2881 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     2941 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     3001 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     3061 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     3121 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     3181 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     3241 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     3301 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     3361 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     3421 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3481 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3541 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3601 caatgtatct tatcatgtct ggatccgtcg accgatgccc ttgagagcct tcaacccagt
     3661 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     3721 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
     3781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4621 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4681 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4741 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4801 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4861 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4921 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4981 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5041 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5101 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5161 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5221 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5281 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5341 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5401 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5461 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5521 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5581 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5641 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
