LOCUS       sequence-275914-        7208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prey vector PSY1R C3_pECIA14 should be used with bait vector PSY1R 
            C3_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: PSY1R C3_pECIA14
FEATURES             Location/Qualifiers
     source          1..7208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2504..2527
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4202..4225
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4241..4258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4281..4298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4331..4465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4355..4374)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4409..4428
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4609..4625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4609..4625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4622..4644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4633..4649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4657..4663,4664..4681,4682..4687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4702..4723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4840..4857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5011..5599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5091..5110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5770..6561,6562..6630))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6393..6412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6631..6735)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6803..6821
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6859..6881)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6981..7000
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(7194..7208,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg cttcaggatc gagattctct
      541 cctttggttc tctggcaacg tatcatctcc agtctctcct ttgcattgga attcgtccat
      601 tgattgttgc tcctgggaag gaatctcttg tgataagtct cctgaaaatc gtgttacttc
      661 gattattttg tcctctagag gactctctgg caatcttccg tcgtctgttt tggatctcca
      721 gcgtctctct cgtctcgacc tttctcataa ccgtctctct ggtccactcc cacctggttt
      781 cctctctgcc cttgatcagc tcttggttct tgatcttagt tacaacagtt tcaaaggtga
      841 attgccactt caacaatcat ttggcaatgg aagcaacgga atcttcccaa ttcagaccgt
      901 tgatctctcc agcaatcttc ttgagggaga aattctcagt agttctgttt ttcttcaggg
      961 tgctttcaat ttaaccagtt tcaatgtcag caacaacagc ttcacgggtt ctatcccttc
     1021 cttcatgtgc acagcttcgc cgcagctcac caaactggat ttctcctaca atgatttcag
     1081 cggtgatctc tctcaagaat taagcagatg ctcgaggcta agtgttcttc gagcaggctt
     1141 caacaatctc tctggtgaaa tccccaaaga aatctacaat cttcctgagc tcgagcaact
     1201 ctttttacca gtcaaccgtc tttctggcaa aattgataat gggattactc gtctcaccaa
     1261 actgacattg cttgagcttt actctaatca catcgaagga gaaataccca aggatatagg
     1321 taagctttcg aaactgagta gcctccaact ccatgtcaat aacctcatgg gttctatccc
     1381 ggtttctctc gccaattgca caaaactcgt gaagttgaat ttacgggtta atcagctggg
     1441 aggaacttta tcggcgatag acttttcccg gtttcagagc ctcagcattc tggacctcgg
     1501 aaacaatagc ttcaccggcg aatttccctc cacggtttac tcatgcaaaa tgatgacagc
     1561 aatgaggttt gcaggcaaca agttaacggg acagatatct cctcaagtac tggaattgga
     1621 atccctgtcg ttctttactt tttcagacaa taaaatgacg aaccttacag gggctctcag
     1681 cattctacaa ggctgcaaga agctttctac tctcattatg gcaaagaatt tttacgacga
     1741 aacggtacca agcaacaaag actttcttcg gtcagatgga tttccaagcc tccaaatttt
     1801 tggtatcggt gcgtgcagat tgacaggtga aataccagct tggctgatca agctccagag
     1861 ggtagaagtt atggacctgt ccatgaaccg attcgtgggg acaattcctg gttggctagg
     1921 aactcttcca gaccttttct acttggatct ttccgataac tttctaacag gagagcttcc
     1981 aaaggaactg tttcagctga gagctctgat gtcgcaaaag gcatacgatg caacagagcg
     2041 gaactatctg gagctgccgg tttttgtcaa tcccaataat gtcaccacta atcagcaata
     2101 caatcagctt tccagcctcc cacctacgat atacatcaag aggaacaact tgactgggac
     2161 tataccagtc gaggttgggc agctaaaggt tctccatatc ctggagcttc taggtaataa
     2221 tttttctggc agcatcccag atgaattgtc aaacctcact aacttagaga ggctggacct
     2281 gtccaacaac aatctctccg gtagaattcc atggtcactc actggactcc atttcctgtc
     2341 atatttcaat gttgcaaaca acactctcag tgggccgata cccacaggga cccagtttga
     2401 taccttccca aaagcaaatt ttgaaggaaa ccccttgctg tgcggtggtg tactgctaac
     2461 ctcctgcgat ccaactcagc actctactac aaagatgggt aaactggagg tgctgttcca
     2521 gggaccaagc ggcaatcccg gcggcgatct ggccccccag atgctgcgcg agctgcagga
     2581 gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc caccgcgtga aggagatcac
     2641 cttcctgaag aacaccgtga tggagtgcga cgcctgcggc atgcagccct ctagaggctc
     2701 cggctccatc atcccagttg aggaggagaa cccggacttc tggaaccgcg aggcagccga
     2761 ggccctgggt gccgccaaga agctgcagcc tgcacagaca gccgccaaga acctcatcat
     2821 cttcctgggc gatgggatgg gggtgtctac ggtgacagct gccaggatcc taaaagggca
     2881 gaagaaggac aaactggggc ctgagatacc cctggccatg gaccgcttcc catatgtggc
     2941 tctgtccaag acatacaatg tagacaaaca tgtgccagac agtggagcca cagccacggc
     3001 ctacctgtgc ggggtcaagg gcaacttcca gaccattggc ttgagtgcag ccgcccgctt
     3061 taaccagtgc aacacgacac gcggcaacga ggtcatctcc gtgatgaatc gggccaagaa
     3121 agcagggaag tcagtgggag tggtaaccac cacacgagtg cagcacgcct cgccagccgg
     3181 cacctacgcc cacacggtga accgcaactg gtactcggac gccgacgtgc ctgcctcggc
     3241 ccgccaggag gggtgccagg acatcgctac gcagctcatc tccaacatgg acattgacgt
     3301 gatcctaggt ggaggccgaa agtacatgtt tcgcatggga accccagacc ctgagtaccc
     3361 agatgactac agccaaggtg ggaccaggct ggacgggaag aatctggtgc aggaatggct
     3421 ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact gagctcatgc aggcttccct
     3481 ggacccgtct gtgacccatc tcatgggtct ctttgagcct ggagacatga aatacgagat
     3541 ccaccgagac tccacactgg acccctccct gatggagatg acagaggctg ccctgcgcct
     3601 gctgagcagg aacccccgcg gcttcttcct cttcgtggag ggtggtcgca tcgaccatgg
     3661 tcatcatgaa agcagggctt accgggcact gactgagacg atcatgttcg acgacgccat
     3721 tgagagggcg ggccagctca ccagcgagga ggacacgctg agcctcgtca ctgccgacca
     3781 ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg agctccatct tcgggctggc
     3841 ccctggcaag gcccgggaca ggaaggccta cacggtcctc ctatacggaa acggtccagg
     3901 ctatgtgctc aaggacggcg cccggccgga tgttaccgag agcgagagcg ggagccccga
     3961 gtatcggcag cagtcagcag tgcccctgga cgaagagacc cacgcaggcg aggacgtggc
     4021 ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc gtgcaggagc agaccttcat
     4081 agcgcacgtc atggccttcg ccgcctgcct ggagccctac accgcctgcg acctggcgcc
     4141 ccccgccggc accaccgacg ccgcgcaccc gggttctaga ggaagcacct ccggcgataa
     4201 cgattacaag gatgatgatg ataaggccag cggcaccggt catcatcacc atcaccattg
     4261 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     4321 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4381 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4441 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     4501 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     4561 tgattatgat cagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt
     4621 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4681 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4741 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4801 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5641 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5701 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5761 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5821 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5881 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5941 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6001 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6061 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     6781 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     6841 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     6901 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     6961 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     7021 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     7081 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     7141 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     7201 acgacgtt
//
